Anaerobic 4-hydroxyproline utilization: Discovery of a new glycyl radical enzyme in the human gut microbiome uncovers a widespread microbial metabolic activity

Huang, Yolanda Y.; Martínez-del Campo, Ana; Balskus, Emily P.

doi:10.1080/19490976.2018.1435244

Anaerobic 4-hydroxyproline utilization: Discovery of a new glycyl radical enzyme in the human gut microbiome uncovers a widespread microbial metabolic activity

Journal Article · Mon Jun 04 00:00:00 EDT 2018 · Gut Microbes

DOI:https://doi.org/10.1080/19490976.2018.1435244· OSTI ID:1903822

^[1]; Martínez-del Campo, Ana ^[2]; Balskus, Emily P. ^[2]

Harvard University, Cambridge, MA (United States); OSTI
Harvard University, Cambridge, MA (United States)

The discovery of enzymes responsible for previously unappreciated microbial metabolic pathways furthers our understanding of host-microbe and microbe-microbe interactions. We recently identified and characterized a new gut microbial glycyl radical enzyme (GRE) responsible for anaerobic metabolism of trans-4-hydroxy-L-proline (Hyp). Hyp dehydratase (HypD) catalyzes the removal of water from Hyp to generate Δ¹ -pyrroline-5-carboxylate (P5C). This enzyme is encoded in the genomes of a diverse set of gut anaerobes and is prevalent and abundant in healthy human stool metagenomes. Here, we discuss the roles HypD may play in different microbial metabolic pathways as well as the potential implications of this activity for colonization resistance and pathogenesis within the human gut. Finally, we present evidence of anaerobic Hyp metabolism in sediments through enrichment culturing of Hyp-degrading bacteria, highlighting the wide distribution of this pathway in anoxic environments beyond the human gut.

View Accepted Manuscript (DOE)

Research Organization:: Harvard University, Cambridge, MA (United States)

Sponsoring Organization:: Abagail Salyers Scholarship Fund; National Aeronautics and Space Administration (NASA); National Science Foundation (NSF); Packard Fellowship for Science and Engineering; Simons Foundation; USDOE

OSTI ID:: 1903822

Journal Information:: Gut Microbes, Journal Name: Gut Microbes Journal Issue: 5 Vol. 9; ISSN 1949-0976

Publisher:: Taylor & FrancisCopyright Statement

Country of Publication:: United States

Language:: English

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