Microbial production of advanced biofuels

Keasling, Jay; Garcia Martin, Hector; Lee, Taek Soon; Mukhopadhyay, Aindrila; Singer, Steven W.; Sundstrom, Eric

doi:10.1038/s41579-021-00577-w

Microbial production of advanced biofuels

Journal Article · Fri Jun 25 00:00:00 EDT 2021 · Nature Reviews Microbiology

DOI:https://doi.org/10.1038/s41579-021-00577-w· OSTI ID:1902663

Keasling, Jay ^[1]; ^[2]; Lee, Taek Soon ^[3]; ^[3]; Singer, Steven W. ^[3]; Sundstrom, Eric ^[4]

Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Technical Univ. of Denmark, Lyngby (Denmark); Shenzhen Institute of Advanced Technology (China)
Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); USDOE Agile BioFoundry, Emeryville, CA (United States); Basque Center for Applied Mathematics (BCAM), Bilbao (Spain)
Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Concerns over climate change have necessitated a rethinking of our transportation infrastructure. One possible alternative to carbon-polluting fossil fuels is biofuels produced by engineered microorganisms that use a renewable carbon source. Two biofuels, ethanol and biodiesel, have made inroads in displacing petroleum-based fuels, but their uptake has been limited by the amounts that can be used in conventional engines and by their cost. Further, advanced biofuels that mimic petroleum-based fuels are not limited by the amounts that can be used in existing transportation infrastructure but have had limited uptake due to costs. In this Review, we discuss engineering metabolic pathways to produce advanced biofuels, challenges with substrate and product toxicity with regard to host microorganisms and methods to engineer tolerance, and the use of functional genomics and machine learning approaches to produce advanced biofuels and prospects for reducing their costs.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office; USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Vehicle Technologies Office; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1902663

Journal Information:: Nature Reviews Microbiology, Journal Name: Nature Reviews Microbiology Journal Issue: 11 Vol. 19; ISSN 1740-1526

Publisher:: Springer NatureCopyright Statement

Country of Publication:: United States

Language:: English

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Restoration of biofuel production levels and increased tolerance under ionic liquid stress is enabled by a mutation in the essential Escherichia coli gene cydC Eng, Thomas; Demling, Philipp; Herbert, Robin Figshare https://doi.org/10.6084/m9.figshare.c.4258877	collection	January 2018
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