A 2-million-year-old ecosystem in Greenland uncovered by environmental DNA

Kjær, Kurt H.; Winther Pedersen, Mikkel; De Sanctis, Bianca; De Cahsan, Binia; Korneliussen, Thorfinn S.; Michelsen, Christian S.; Sand, Karina K.; Jelavić, Stanislav; Ruter, Anthony H.; Schmidt, Astrid A.; Kjeldsen, Kristian K.; Tesakov, Alexey S.; Snowball, Ian; Gosse, John C.; Alsos, Inger G.; Wang, Yucheng; Dockter, Christoph; Rasmussen, Magnus; Jørgensen, Morten E.; Skadhauge, Birgitte; Prohaska, Ana; Kristensen, Jeppe Å.; Bjerager, Morten; Allentoft, Morten E.; Coissac, Eric; Alsos, Inger Greve; Rouillard, Alexandra; Simakova, Alexandra; Fernandez-Guerra, Antonio; Bowler, Chris; Macias-Fauria, Marc; Vinner, Lasse; Welch, John J.; Hidy, Alan J.; Sikora, Martin; Collins, Matthew J.; Durbin, Richard; Larsen, Nicolaj K.; Willerslev, Eske

doi:10.1038/s41586-022-05453-y

A 2-million-year-old ecosystem in Greenland uncovered by environmental DNA

Journal Article · Wed Dec 07 00:00:00 EST 2022 · Nature (London)

DOI:https://doi.org/10.1038/s41586-022-05453-y· OSTI ID:1902598

^[1]; ^[1]; ^[2]; ^[3]; Korneliussen, Thorfinn S. ^[1]; Michelsen, Christian S. ^[1]; ^[1]; ^[4]; Ruter, Anthony H. ^[1]; Schmidt, Astrid A. ^[5]; ^[6]; Tesakov, Alexey S. ^[7]; Snowball, Ian ^[8]; ^[9]; ^[10]; ^[11]; ^[12]; ^[12]; ^[12]; ^[12] more »

University of Copenhagen (Denmark)
University of Cambridge (United Kingdom)
Globe Institute, Copenhagen (Denmark)
University of Copenhagen (Denmark); University of Grenoble Alpes, Grenoble (France); Université Savoie Mont Blanc, Grenoble (France); Université Gustave Eiffel, Grenoble (France)
Nordic Foundation for Development and Ecology (NORDECO), Copenhagen (Denmark); University of Copenhagen (Denmark)
Geological Survey of Denmark and Greenland, Copenhagen (Denmark)
Russian Academy of Sciences (RAS), Moscow (Russian Federation)
Uppsala University (Sweden)
Dalhousie University, Halifax, NS (Canada)
UiT The Arctic University of Norway, Tromso (Norway)
University of Copenhagen (Denmark); University of Cambridge (United Kingdom)
Carlsberg Research Laboratory, Copenhagen (Denmark)
University of Oxford (United Kingdom)
University of Copenhagen (Denmark); Curtin University, Perth, WA (Australia)
UiT The Arctic University of Norway, Tromso (Norway); University of Grenoble Alpes, Grenoble (France); Université Savoie Mont Blanc, Grenoble (France); Université PSL, Paris (France)
University of Copenhagen (Denmark); UiT The Arctic University of Norway, Tromso (Norway)
Université PSL, Paris (France)
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
University of Copenhagen (Denmark); University of Cambridge (United Kingdom); University of Bremen (Germany)

Late Pliocene and Early Pleistocene epochs 3.6 to 0.8 million years ago had climates resembling those forecasted under future warming. Palaeoclimatic records show strong polar amplification with mean annual temperatures of 11–19 °C above contemporary values. The biological communities inhabiting the Arctic during this time remain poorly known because fossils are rare. Here we report an ancient environmental DNA (eDNA) record describing the rich plant and animal assemblages of the Kap København Formation in North Greenland, dated to around two million years ago. The record shows an open boreal forest ecosystem with mixed vegetation of poplar, birch and thuja trees, as well as a variety of Arctic and boreal shrubs and herbs, many of which had not previously been detected at the site from macrofossil and pollen records. The DNA record confirms the presence of hare and mitochondrial DNA from animals including mastodons, reindeer, rodents and geese, all ancestral to their present-day and late Pleistocene relatives. The presence of marine species including horseshoe crab and green algae support a warmer climate than today. The reconstructed ecosystem has no modern analogue. The survival of such ancient eDNA probably relates to its binding to mineral surfaces. Our findings open new areas of genetic research, demonstrating that it is possible to track the ecology and evolution of biological communities from two million years ago using ancient eDNA.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: Carlsberg Foundation; Danish National Research Foundation (DNRF); European Research Council (ERC); GRF EXC CRS Chair - Cluster of Excellence; Leverhume; Novo Foundation; USDOE National Nuclear Security Administration (NNSA); Villum Fonden; Wellcome Trust

Grant/Contract Number:: AC52-07NA27344

OSTI ID:: 1902598

Report Number(s):: LLNL-JRNL-830653; 1047300

Journal Information:: Nature (London), Journal Name: Nature (London) Journal Issue: 7939 Vol. 612; ISSN 0028-0836

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

References (81)

Raster Circumpolar Arctic Vegetation Map Raynolds, Martha Mendeley https://doi.org/10.17632/c4xj5rv6kv	dataset	January 2022
Plastome phylogeny and lineage diversification of Salicaceae with focus on poplars and willows Zhang, Lei; Xi, Zhenxiang; Wang, Mingcheng Ecology and Evolution, Vol. 8, Issue 16 https://doi.org/10.1002/ece3.4261	journal	July 2018
Fire history in a western Fennoscandian boreal forest as influenced by human land use and climate Rolstad, Jørund; Blanck, Ylva-li; Storaunet, Ken Olaf Ecological Monographs, Vol. 87, Issue 2 https://doi.org/10.1002/ecm.1244	journal	February 2017
Driving Forces for DNA Adsorption to Silica in Perchlorate Solutions Melzak, Kathryn A.; Sherwood, Chris S.; Turner, Robin F. B. Journal of Colloid and Interface Science, Vol. 181, Issue 2 https://doi.org/10.1006/jcis.1996.0421	journal	August 1996
Magnetostratigraphy of the Plio-Pleistocene Kap København Formation, eastern North Greenland Abrahamsen, Niels; Marcussen, Christian Physics of the Earth and Planetary Interiors, Vol. 44, Issue 1 https://doi.org/10.1016/0031-9201(86)90114-7	journal	September 1986
Environmental genomics of Late Pleistocene black bears and giant short-faced bears Pedersen, Mikkel Winther; De Sanctis, Bianca; Saremi, Nedda F. Current Biology, Vol. 31, Issue 12 https://doi.org/10.1016/j.cub.2021.04.027	journal	June 2021
Amplified Late Pliocene terrestrial warmth in northern high latitudes from greater radiative forcing and closed Arctic Ocean gateways Feng, Ran; Otto-Bliesner, Bette L.; Fletcher, Tamara L. Earth and Planetary Science Letters, Vol. 466 https://doi.org/10.1016/j.epsl.2017.03.006	journal	May 2017
Last Glacial Maximum environmental conditions at Andøya, northern Norway; evidence for a northern ice-edge ecological “hotspot” Alsos, Inger G.; Sjögren, Per; Brown, Antony G. Quaternary Science Reviews, Vol. 239 https://doi.org/10.1016/j.quascirev.2020.106364	journal	July 2020
A raster version of the Circumpolar Arctic Vegetation Map (CAVM) Raynolds, Martha K.; Walker, Donald A.; Balser, Andrew Remote Sensing of Environment, Vol. 232 https://doi.org/10.1016/j.rse.2019.111297	journal	October 2019
Functional repertoire convergence of distantly related eukaryotic plankton lineages abundant in the sunlit ocean Delmont, Tom O.; Gaia, Morgan; Hinsinger, Damien D. Cell Genomics, Vol. 2, Issue 5 https://doi.org/10.1016/j.xgen.2022.100123	journal	May 2022
Optimizing extraction and targeted capture of ancient environmental DNA for reconstructing past environments using the PalaeoChip Arctic-1.0 bait-set Murchie, Tyler J.; Kuch, Melanie; Duggan, Ana T. Quaternary Research, Vol. 99 https://doi.org/10.1017/qua.2020.59	journal	September 2020
Rate of depurination of native deoxyribonucleic acid Lindahl, Tomas; Nyberg, Barbro Biochemistry, Vol. 11, Issue 19 https://doi.org/10.1021/bi00769a018	journal	September 1972
Plasmid DNA Adsorption on Silica: Kinetics and Conformational Changes in Monovalent and Divalent Salts Nguyen, Thanh H.; Elimelech, Menachem Biomacromolecules, Vol. 8, Issue 1 https://doi.org/10.1021/bm0603948	journal	December 2006
Interactions of DNA with Clay Minerals and Soil Colloidal Particles and Protection against Degradation by DNase Cai, Peng; Huang, Qiao-Yun; Zhang, Xue-Wen Environmental Science & Technology, Vol. 40, Issue 9 https://doi.org/10.1021/es0522985	journal	May 2006
Early Intermediates in Spermidine-Induced DNA Condensation on the Surface of Mica Fang, Ye; Hoh, Jan H. Journal of the American Chemical Society, Vol. 120, Issue 35 https://doi.org/10.1021/ja981332v	journal	August 1998
Middle Pliocene sea surface temperature variability Dowsett, Harry J.; Chandler, Mark A.; Cronin, Thomas M. Paleoceanography, Vol. 20, Issue 2 https://doi.org/10.1029/2005PA001133	journal	June 2005
Instability and decay of the primary structure of DNA Lindahl, Tomas Nature, Vol. 362, Issue 6422 https://doi.org/10.1038/362709a0	journal	April 1993
Postglacial viability and colonization in North America’s ice-free corridor Pedersen, Mikkel W.; Ruter, Anthony; Schweger, Charles Nature, Vol. 537, Issue 7618 https://doi.org/10.1038/nature19085	journal	August 2016
Mid-Pliocene warm-period deposits in the High Arctic yield insight into camel evolution Rybczynski, Natalia; Gosse, John C.; Richard Harington, C. Nature Communications, Vol. 4, Issue 1 https://doi.org/10.1038/ncomms2516	journal	March 2013
Fast gapped-read alignment with Bowtie 2 Langmead, Ben; Salzberg, Steven L. Nature Methods, Vol. 9, Issue 4 https://doi.org/10.1038/nmeth.1923	journal	March 2012
American mastodon mitochondrial genomes suggest multiple dispersal events in response to Pleistocene climate oscillations Karpinski, Emil; Hackenberger, Dirk; Zazula, Grant Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-17893-z	journal	September 2020
Community-led, integrated, reproducible multi-omics with anvi’o Eren, A. Murat; Kiefl, Evan; Shaiber, Alon Nature Microbiology, Vol. 6, Issue 1 https://doi.org/10.1038/s41564-020-00834-3	journal	December 2020
Evidence of human occupation in Mexico around the Last Glacial Maximum Ardelean, Ciprian F.; Becerra-Valdivia, Lorena; Pedersen, Mikkel Winther Nature, Vol. 584, Issue 7819 https://doi.org/10.1038/s41586-020-2509-0	journal	July 2020
Million-year-old DNA sheds light on the genomic history of mammoths van der Valk, Tom; Pečnerová, Patrícia; Díez-del-Molino, David Nature, Vol. 591, Issue 7849 https://doi.org/10.1038/s41586-021-03224-9	journal	February 2021
Late Quaternary dynamics of Arctic biota from ancient environmental genomics Wang, Yucheng; Pedersen, Mikkel Winther; Alsos, Inger Greve Nature, Vol. 600, Issue 7887 https://doi.org/10.1038/s41586-021-04016-x	journal	October 2021
A basal ursine bear (Protarctos abstrusus) from the Pliocene High Arctic reveals Eurasian affinities and a diet rich in fermentable sugars Wang, Xiaoming; Rybczynski, Natalia; Harington, C. Richard Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/s41598-017-17657-8	journal	December 2017
An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG II Group*, Angiosperm Phylogeny Botanical Journal of the Linnean Society, Vol. 141, Issue 4, p. 399-436 https://doi.org/10.1046/j.1095-8339.2003.t01-1-00158.x	journal	January 2003
Megafauna and ecosystem function from the Pleistocene to the Anthropocene Malhi, Yadvinder; Doughty, Christopher E.; Galetti, Mauro Proceedings of the National Academy of Sciences, Vol. 113, Issue 4 https://doi.org/10.1073/pnas.1502540113	journal	January 2016
Timing and causes of mid-Holocene mammoth extinction on St. Paul Island, Alaska Graham, Russell W.; Belmecheri, Soumaya; Choy, Kyungcheol Proceedings of the National Academy of Sciences, Vol. 113, Issue 33 https://doi.org/10.1073/pnas.1604903113	journal	August 2016
Biopython: freely available Python tools for computational molecular biology and bioinformatics Cock, P. J. A.; Antao, T.; Chang, J. T. Bioinformatics, Vol. 25, Issue 11 https://doi.org/10.1093/bioinformatics/btp163	journal	March 2009
Fast and accurate short read alignment with Burrows-Wheeler transform Li, H.; Durbin, R. Bioinformatics, Vol. 25, Issue 14 https://doi.org/10.1093/bioinformatics/btp324	journal	May 2009
The Sequence Alignment/Map format and SAMtools Li, H.; Handsaker, B.; Wysoker, A. Bioinformatics, Vol. 25, Issue 16 https://doi.org/10.1093/bioinformatics/btp352	journal	June 2009
Improving SNP discovery by base alignment quality Li, H. Bioinformatics, Vol. 27, Issue 8 https://doi.org/10.1093/bioinformatics/btr076	journal	February 2011
MSAViewer: interactive JavaScript visualization of multiple sequence alignments Yachdav, Guy; Wilzbach, Sebastian; Rauscher, Benedikt Bioinformatics https://doi.org/10.1093/bioinformatics/btw474	journal	July 2016
Placing Ancient DNA Sequences into Reference Phylogenies Martiniano, Rui; De Sanctis, Bianca; Hallast, Pille Molecular Biology and Evolution, Vol. 39, Issue 2 https://doi.org/10.1093/molbev/msac017	journal	January 2022
A Bayesian Phylogenetic Method to Estimate Unknown Sequence Ages Shapiro, B.; Ho, S. Y. W.; Drummond, A. J. Molecular Biology and Evolution, Vol. 28, Issue 2 https://doi.org/10.1093/molbev/msq262	journal	October 2010
MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability Katoh, K.; Standley, D. M. Molecular Biology and Evolution, Vol. 30, Issue 4 https://doi.org/10.1093/molbev/mst010	journal	January 2013
MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms Kumar, Sudhir; Stecher, Glen; Li, Michael Molecular Biology and Evolution, Vol. 35, Issue 6 https://doi.org/10.1093/molbev/msy096	journal	May 2018
GenBank Clark, Karen; Karsch-Mizrachi, Ilene; Lipman, David J. Nucleic Acids Research, Vol. 44, Issue D1 https://doi.org/10.1093/nar/gkv1276	journal	November 2015
Pleistocene Speciation in the Genus Populus (Salicaceae) Levsen, Nicholas D.; Tiffin, Peter; Olson, Matthew S. Systematic Biology, Vol. 61, Issue 3 https://doi.org/10.1093/sysbio/syr120	journal	February 2012
Posterior Summarization in Bayesian Phylogenetics Using Tracer 1.7 Rambaut, Andrew; Drummond, Alexei J.; Xie, Dong Systematic Biology, Vol. 67, Issue 5 https://doi.org/10.1093/sysbio/syy032	journal	April 2018
Climate sensitivity, sea level and atmospheric carbon dioxide Hansen, James; Sato, Makiko; Russell, Gary Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, Vol. 371, Issue 2001 https://doi.org/10.1098/rsta.2012.0294	journal	October 2013
Illumina Sequencing Library Preparation for Highly Multiplexed Target Capture and Sequencing Meyer, M.; Kircher, M. Cold Spring Harbor Protocols, Vol. 2010, Issue 6 https://doi.org/10.1101/pdb.prot5448	journal	June 2010
A fundamental parameters approach to X-ray line-profile fitting Cheary, R. W.; Coelho, A. Journal of Applied Crystallography, Vol. 25, Issue 2 https://doi.org/10.1107/S0021889891010804	journal	April 1992
Profex : a graphical user interface for the Rietveld refinement program BGMN Doebelin, Nicola; Kleeberg, Reinhard Journal of Applied Crystallography, Vol. 48, Issue 5 https://doi.org/10.1107/S1600576715014685	journal	August 2015
ngs LCA —A toolkit for fast and flexible lowest common ancestor inference and taxonomic profiling of metagenomic data Wang, Yucheng; Korneliussen, Thorfinn Sand; Holman, Luke E. Methods in Ecology and Evolution, Vol. 13, Issue 12 https://doi.org/10.1111/2041-210X.14006	journal	October 2022
Ecological and evolutionary legacy of megafauna extinctions: Anachronisms and megafauna interactions Galetti, Mauro; Moleón, Marcos; Jordano, Pedro Biological Reviews, Vol. 93, Issue 2 https://doi.org/10.1111/brv.12374	journal	October 2017
Macrofossils in an interglacial peat deposit at Kap København, North Greenland Fredskild, Bent; RØEn, Ulrik Boreas, Vol. 11, Issue 3 https://doi.org/10.1111/j.1502-3885.1982.tb00713.x	journal	January 2008
Plastomes of Betulaceae and phylogenetic implications Yang, Xiao‐Yue; Wang, Ze‐Fu; Luo, Wen‐Chun Journal of Systematics and Evolution, Vol. 57, Issue 5 https://doi.org/10.1111/jse.12479	journal	February 2019
Whole plastome sequencing reveals deep plastid divergence and cytonuclear discordance between closely related balsam poplars, P opulus balsamifera and P . trichocarpa ( S alicaceae) Huang, Daisie I.; Hefer, Charles A.; Kolosova, Natalia New Phytologist, Vol. 204, Issue 3 https://doi.org/10.1111/nph.12956	journal	July 2014
Ancient plant DNA in lake sediments Parducci, Laura; Bennett, Keith D.; Ficetola, Gentile Francesco New Phytologist, Vol. 214, Issue 3 https://doi.org/10.1111/nph.14470	journal	April 2017
Sedimentary ancient DNA shows terrestrial plant richness continuously increased over the Holocene in northern Fennoscandia Rijal, Dilli P.; Heintzman, Peter D.; Lammers, Youri Science Advances, Vol. 7, Issue 31 https://doi.org/10.1126/sciadv.abf9557	journal	July 2021
Diverse Plant and Animal Genetic Records from Holocene and Pleistocene Sediments Willerslev, Eske; Hansen, Anders J.; Binladen, Jonas Science, Vol. 300, Issue 5620 https://doi.org/10.1126/science.1084114	journal	May 2003
Pliocene Warmth, Polar Amplification, and Stepped Pleistocene Cooling Recorded in NE Arctic Russia Brigham-Grette, J.; Melles, M.; Minyuk, P. Science, Vol. 340, Issue 6139 https://doi.org/10.1126/science.1233137	journal	May 2013
Forested Arctic: Evidence from North Greenland Funder, Svend; Abrahamsen, Niels; Bennike, Ole Geology, Vol. 13, Issue 8 https://doi.org/10.1130/0091-7613(1985)13<542:FAEFNG>2.0.CO;2	journal	January 1985
Dung analysis of the East Milford mastodons: dietary and environmental reconstructions from central Nova Scotia at ∼75 ka years BP Cocker, Scott L.; Pisaric, Michael F. J.; McCarthy, Francine M. G. Canadian Journal of Earth Sciences, Vol. 58, Issue 10 https://doi.org/10.1139/cjes-2020-0164	journal	October 2021
Arctic North American seasonal temperatures from the latest Miocene to the Early Pleistocene, based on mutual climatic range analysis of fossil beetle assemblages Elias, Scott A.; Matthews Jr., John V. Canadian Journal of Earth Sciences, Vol. 39, Issue 6 https://doi.org/10.1139/e01-096	journal	June 2002
BEAST: Bayesian evolutionary analysis by sampling trees Drummond, Alexei J.; Rambaut, Andrew BMC Evolutionary Biology, Vol. 7, Issue 1 https://doi.org/10.1186/1471-2148-7-214	journal	January 2007
Improved metagenomic analysis with Kraken 2 Wood, Derrick E.; Lu, Jennifer; Langmead, Ben Genome Biology, Vol. 20, Issue 1 https://doi.org/10.1186/s13059-019-1891-0	journal	November 2019
Seqotron: a user-friendly sequence editor for Mac OS X Fourment, Mathieu; Holmes, Edward C. BMC Research Notes, Vol. 9, Issue 1 https://doi.org/10.1186/s13104-016-1927-4	journal	February 2016
PoLAR-FIT: Pliocene Landscapes and Arctic Remains—Frozen in Time Gosse, John C.; Ballantyne, Ashley P.; Barker, Joel D. Geoscience Canada, Vol. 44, Issue 1 https://doi.org/10.12789/geocanj.2017.44.116	journal	April 2017
A Holistic Approach to Marine Eco-Systems Biology Karsenti, Eric; Acinas, Silvia G.; Bork, Peer PLoS Biology, Vol. 9, Issue 10 https://doi.org/10.1371/journal.pbio.1001177	journal	October 2011
BEAST 2: A Software Platform for Bayesian Evolutionary Analysis Bouckaert, Remco; Heled, Joseph; Kühnert, Denise PLoS Computational Biology, Vol. 10, Issue 4 https://doi.org/10.1371/journal.pcbi.1003537	journal	April 2014
Plant DNA metabarcoding of lake sediments: How does it represent the contemporary vegetation Alsos, Inger Greve; Lammers, Youri; Yoccoz, Nigel Giles PLOS ONE, Vol. 13, Issue 4 https://doi.org/10.1371/journal.pone.0195403	journal	April 2018
Forest-Tundra Neighbouring the North Pole: Plant and Insect Remains from the Plio-Pleistocene Kap København Formation, North Greenland Bennike, O.; Böcher, J. ARCTIC, Vol. 43, Issue 4 https://doi.org/10.14430/arctic1629	journal	January 1990
Floristical and ecological characterization of the polar desert zone of Greenland Bay, Christian Journal of Vegetation Science, Vol. 8, Issue 5 https://doi.org/10.2307/3237373	journal	October 1997
Neogene and early Pleistocene flora from Alaska and Arctic/Subarctic Canada: New data, intercontinental comparisons and correlations Fletcher, Tamara; Telka, Alice; Rybczynski, Natalia Palaeontologia Electronica https://doi.org/10.26879/1121	journal	January 2021
Shotgun Environmental DNA, Pollen, and Macrofossil Analysis of Lateglacial Lake Sediments From Southern Sweden Parducci, Laura; Alsos, Inger Greve; Unneberg, Per Frontiers in Ecology and Evolution, Vol. 7 https://doi.org/10.3389/fevo.2019.00189	journal	June 2019
Phylogenomic Analysis and Dynamic Evolution of Chloroplast Genomes in Salicaceae Huang, Yuan; Wang, Jun; Yang, Yongping Frontiers in Plant Science, Vol. 8 https://doi.org/10.3389/fpls.2017.01050	journal	June 2017
DNA Repair and the Stability of the Plant Mitochondrial Genome Chevigny, Nicolas; Schatz-Daas, Déborah; Lotfi, Frédérique International Journal of Molecular Sciences, Vol. 21, Issue 1 https://doi.org/10.3390/ijms21010328	journal	January 2020
A remarkable foraminiferal assemblage from the Quaternary of northeast Greenland Hanssen, R. W. Feyling Bulletin of the Geological Society of Denmark, Vol. 38 https://doi.org/10.37570/bgsd-1990-38-10	journal	April 1990
Four decades of new vascular plant records for Greenland Bay, Christian PhytoKeys, Vol. 145 https://doi.org/10.3897/phytokeys.145.39704	journal	April 2020
Preparation of Artificial Seawater1 Kester, Dana R.; Duedall, Iver W.; Connors, Donald N. Limnology and Oceanography, Vol. 12, Issue 1 https://doi.org/10.4319/lo.1967.12.1.0176	journal	April 1967
The measurement and distribution of dissolved nucleic acids in aquatic environments: Dissolved DNA and RNA Karl, David M.; Bailiff, Megan D. Limnology and Oceanography, Vol. 34, Issue 3 https://doi.org/10.4319/lo.1989.34.3.0543	journal	May 1989
Glacier changes and climate trends derived from multiple sources in the data scarce Cordillera Vilcanota region, southern Peruvian Andes Salzmann, N.; Huggel, C.; Rohrer, M. The Cryosphere, Vol. 7, Issue 1 https://doi.org/10.5194/tc-7-103-2013	journal	January 2013
A phytogeographical study of the vascular plants of northern Greenland - north of 74° northern latitude Bay, Christian Meddelelser om Grønland. Bioscience, Vol. 36 https://doi.org/10.7146/mogbiosci.v36.142523	journal	December 1992
The Kap København Formation: stratigraphy and palaeobotany of a Plio-Pleistocene sequence in Peary Land, North Greenland Bennike, Ole Meddelelser om Grønland. Geoscience, Vol. 23 https://doi.org/10.7146/moggeosci.v23i.141978	journal	December 1990
Foraminiferal stratigraphy in the Plio-Pleistocene Kap Kebenhavn Formation, North Greenland Feyling-Hanssen, Rolf W. Meddelelser om Grønland. Geoscience, Vol. 24 https://doi.org/10.7146/moggeosci.v24i.142019	journal	January 1991
Palaeoentomology of the Kap København Formation, a Plio-Pleistocene sequence in Peary Land, North Greenland Böcher, Jens Meddelelser om Grønland. Geoscience, Vol. 33 https://doi.org/10.7146/moggeosci.v33i.140906	journal	January 1995
Molluscan palaeontology of the Pliocene-Pleistocene Kap København Formation, North Greenland Símonarson, Leifur A.; Petersen, Kaj Strand; Funder, Svend Meddelelser om Grønland. Geoscience, Vol. 36 https://doi.org/10.7146/moggeosci.v36i.140966	journal	February 1998
Multiple comparative metagenomics using multiset k -mer counting Benoit, Gaëtan; Peterlongo, Pierre; Mariadassou, Mahendra PeerJ Computer Science, Vol. 2 https://doi.org/10.7717/peerj-cs.94	journal	January 2016

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