Subsurface microbial communities as a tool for characterizing regional-scale groundwater flow

Merino, Nancy; Jackson, Tracie R.; Campbell, James H.; Kersting, Annie B.; Sackett, Joshua; Fisher, Jenny C.; Bruckner, James C.; Zavarin, Mavrik; Hamilton-Brehm, Scott D.; Moser, Duane P.

doi:10.1016/j.scitotenv.2022.156768

Subsurface microbial communities as a tool for characterizing regional-scale groundwater flow

Journal Article · Tue Jun 21 00:00:00 EDT 2022 · Science of the Total Environment

DOI:https://doi.org/10.1016/j.scitotenv.2022.156768· OSTI ID:1902010

Merino, Nancy ^[1]; Jackson, Tracie R. ^[2]; Campbell, James H. ^[3]; Kersting, Annie B. ^[1]; Sackett, Joshua ^[4]; Fisher, Jenny C. ^[5]; Bruckner, James C. ^[6]; Zavarin, Mavrik ^[1]; Hamilton-Brehm, Scott D. ^[7]; Moser, Duane P. ^[6]

Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
US Geological Survey, Boulder, CO (United States)
Northwest Missouri State University, Maryville, MO (United States)
Desert Research Institute (DRI), Las Vegas, NV (United States); University of Nevada, Las Vegas, NV (United States)
Desert Research Institute (DRI), Las Vegas, NV (United States); Indiana University Northwets, Gary, IN (United States)
Desert Research Institute (DRI), Las Vegas, NV (United States)
Desert Research Institute (DRI), Las Vegas, NV (United States); Southern Illinois University, Carbondale, IL (United States)

Subsurface microbial community distribution patterns are influenced by biogeochemical and groundwater fluxes and may inform hydraulic connections along groundwater-flow paths. This study examined the regional-scale microbial community of the Death Valley Regional Flow System and evaluated whether subsurface communities can be used to identify groundwater-flow paths between recharge and discharge areas. Samples were collected from 36 sites in three groundwater basins: Pahute Mesa–Oasis Valley (PMOV), Ash Meadows (AM), and Alkali Flat–Furnace Creek Ranch (AFFCR). Microbial diversity within and between communities varied by location, and communities were separated into two overall groups that affiliated with the AM and PMOV/AFFCR basins. Network analysis revealed patterns between clusters of common microbes that represented groundwaters with similar geochemical conditions and largely corroborated hydraulic connections between recharge and discharge areas. Null model analyses identified deterministic and stochastic ecological processes contributing to microbial community assemblages. Most communities were more different than expected and governed by dispersal limitation, geochemical differences, or undominating processes. However, certain communities from sites located within or near the Nevada National Security Site were more similar than expected and dominated by homogeneous dispersal or selection. Overall, the (dis)similarities between the microbial communities of DVRFS recharge and discharge areas supported previously documented hydraulic connections between: (1) Spring Mountains and Ash Meadows; (2) Frenchman and Yucca Flat and Amargosa Desert; and (3) Amargosa Desert and Death Valley. However, only a portion of the flow path between Pahute Mesa and Oasis Valley could be supported by microbial community analyses, likely due to well-associated artifacts in samples from the two Oasis Valley sites. This study demonstrates the utility of combining microbial data with hydrologic, geologic, and water-chemistry information to comprehensively characterize groundwater systems, highlighting both strengths and limitations of this approach.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA); USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); National Aeronautics and Space Administration (NASA)

Grant/Contract Number:: AC52-07NA27344; SC0005306; FG02-07ER64406

OSTI ID:: 1902010

Report Number(s):: LLNL-JRNL-823248; 1035849

Journal Information:: Science of the Total Environment, Journal Name: Science of the Total Environment Journal Issue: N/A Vol. 842; ISSN 0048-9697

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Subsurface microbial communities as a tool for characterizing regional-scale groundwater flow

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