An efficient and broadly applicable method for transient transformation of plants using vertically aligned carbon nanofiber arrays

Morgan, Jessica M.; Jelenska, Joanna; Hensley, Dale; Retterer, Scott T.; Morrell-Falvey, Jennifer L.; Standaert, Robert F.; Greenberg, Jean T.

doi:10.3389/fpls.2022.1051340

Title: An efficient and broadly applicable method for transient transformation of plants using vertically aligned carbon nanofiber arrays

Journal Article · Wed Nov 23 00:00:00 EST 2022 · Frontiers in Plant Science

DOI:https://doi.org/10.3389/fpls.2022.1051340· OSTI ID:1899712

Morgan, Jessica M.; Jelenska, Joanna; Hensley, Dale; Retterer, Scott T.; Morrell-Falvey, Jennifer L.; Standaert, Robert F.; Greenberg, Jean T.

Transient transformation in plants is a useful process for evaluating gene function. However, there is a scarcity of minimally perturbing methods for gene delivery that can be used on multiple organs, plant species, and non-excised tissues. We pioneered and demonstrated the use of vertically aligned carbon nanofiber (VACNF) arrays to efficiently perform transient transformation of different tissues with DNA constructs in multiple plant species. The VACNFs permeabilize plant tissue transiently to allow molecules into cells without causing a detectable stress response. We successfully delivered DNA into leaves, roots and fruit of five plant species (Arabidopsis, poplar, lettuce, Nicotiana benthamiana, and tomato) and confirmed accumulation of the encoded fluorescent proteins by confocal microscopy. Using this system, it is possible to transiently transform plant cells with both small and large plasmids. The method is successful for species recalcitrant to Agrobacterium-mediated transformation. VACNFs provide simple, reliable means of DNA delivery into a variety of plant organs and species.

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Research Organization:: Univ. of Chicago, IL (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDA, National Institute of Food and Agriculture (NIFA). Agriculture and Food Research Initiative (AFRI)

Grant/Contract Number:: SC0019104; AC05-00OR22725

OSTI ID:: 1899712

Alternate ID(s):: OSTI ID: 1903369; OSTI ID: 1905379

Journal Information:: Frontiers in Plant Science, Journal Name: Frontiers in Plant Science Vol. 13; ISSN 1664-462X

Publisher:: Frontiers Media SACopyright Statement

Country of Publication:: Switzerland

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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vertically aligned carbon nanofibers (VACNF)
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Title: An efficient and broadly applicable method for transient transformation of plants using vertically aligned carbon nanofiber arrays

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References (49)

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