Characterization of lewisite toxicity in isolated perfused skin. (Reannouncement with new availability information)
Lewisite (L) is a potent organic arsenical that causes rapid onset of pain and severe vesication on contact with epithelial tissues. The isolated perfused porcine skin flap (IPPSF) is an in vitro model that has shown potential as a model for cutaneous vesicant research. The objective of this study was to characterize IPPSF responses after topical exposure to six concentrations of L ranging from 0.07 to 5.0 mg/ml (n = 4/treatment plus controls). Biochemical markers of viability (glucose utilization (CGU) and lactate dehydrogenase (LDH) release), vascular resistance (VR), venous arsenic flux, and morphological parameters (light and electron microscopy) were evaluated. In addition, lewisite lesions were characterized at 1, 3, 5, and 8 hr after exposure (n = 4/time plus controls) using these morphological parameters. as well as enzyme histochemistry. Macroscopic and microscopic lesions caused by L exposure were dose related.
- Research Organization:
- North Carolina State Univ., Raleigh, NC (United States)
- OSTI ID:
- 189773
- Report Number(s):
- AD-A--257910/0/XAB; CNN: Contract DAMD17-87-C-7139
- Country of Publication:
- United States
- Language:
- English
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