Metagenomes and Metagenome-Assembled Genomes from Substrate-Amended Hot Spring Sediment Incubations from Yellowstone National Park

Reichart, Nicholas J.; Bowers, Robert M.; Woyke, Tanja; Hatzenpichler, Roland

doi:10.1128/mra.01065-21

Metagenomes and Metagenome-Assembled Genomes from Substrate-Amended Hot Spring Sediment Incubations from Yellowstone National Park

Journal Article · Wed Mar 30 00:00:00 EDT 2022 · Microbiology Resource Announcements

DOI:https://doi.org/10.1128/mra.01065-21· OSTI ID:1894079

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Montana State Univ., Bozeman, MT (United States)
USDOE Joint Genome Institute (JGI), Berkeley, CA (United States)

Here, we report on eight sediment metagenomes obtained from an alkaline hot spring, with their corresponding metagenome-assembled genomes. Samples had been incubated for 48 h with various substrate amendments in conjunction with the amino acid analog l-homopropargylglycine in a study targeted at identifying anabolicly active uncultured thermophilic archaea and bacteria.

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); National Aeronautics and Space Administration (NASA); USDOE Office of Science (SC), Workforce Development for Teachers and Scientists (WDTS)

Grant/Contract Number:: AC02-05CH11231; SC0014664

OSTI ID:: 1894079

Journal Information:: Microbiology Resource Announcements, Journal Name: Microbiology Resource Announcements Journal Issue: 4 Vol. 11; ISSN 2576-098X

Publisher:: SSPA - American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

References (9)

Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea Bowers, Robert M.; Kyrpides, Nikos C.; Stepanauskas, Ramunas Nature Biotechnology, Vol. 35, Issue 8 https://doi.org/10.1038/nbt.3893	journal	August 2017
Global metagenomic survey reveals a new bacterial candidate phylum in geothermal springs Eloe-Fadrosh, Emiley A.; Paez-Espino, David; Jarett, Jessica Nature Communications, Vol. 7, Issue 1 https://doi.org/10.1038/ncomms10476	journal	January 2016
Activity-based cell sorting reveals responses of uncultured archaea and bacteria to substrate amendment Reichart, Nicholas J.; Jay, Zackary J.; Krukenberg, Viola The ISME Journal, Vol. 14, Issue 11 https://doi.org/10.1038/s41396-020-00749-1	journal	September 2020
Next-generation physiology approaches to study microbiome function at single cell level Hatzenpichler, Roland; Krukenberg, Viola; Spietz, Rachel L. Nature Reviews Microbiology, Vol. 18, Issue 4 https://doi.org/10.1038/s41579-020-0323-1	journal	February 2020
Visualizing in situ translational activity for identifying and sorting slow-growing archaeal−bacterial consortia Hatzenpichler, Roland; Connon, Stephanie A.; Goudeau, Danielle Proceedings of the National Academy of Sciences, Vol. 113, Issue 28 https://doi.org/10.1073/pnas.1603757113	journal	June 2016
The IMG/M data management and analysis system v.6.0: new tools and advanced capabilities Chen, I-Min A.; Chu, Ken; Palaniappan, Krishnaveni Nucleic Acids Research, Vol. 49, Issue D1 https://doi.org/10.1093/nar/gkaa939	journal	October 2020
In situ visualization of newly synthesized proteins in environmental microbes using amino acid tagging and click chemistry : Visualizing new proteins in microbes by click chemistry Hatzenpichler, Roland; Scheller, Silvan; Tavormina, Patricia L. Environmental Microbiology, Vol. 16, Issue 8 https://doi.org/10.1111/1462-2920.12436	journal	April 2014
Phylogenetically Novel Uncultured Microbial Cells Dominate Earth Microbiomes Lloyd, Karen G.; Steen, Andrew D.; Ladau, Joshua mSystems, Vol. 3, Issue 5 https://doi.org/10.1128/mSystems.00055-18	journal	September 2018
SOI tables for Reichart et al. 2022 Microbiology Research Announcements Reichart, Nicholas J.; Bowers, Robert M.; Hatzenpichler, Roland figshare https://doi.org/10.6084/m9.figshare.16905058.v5	dataset	January 2022

Cited By (1)

Additional file 1 of Integrating multi-platform assembly to recover MAGs from hot spring biofilms: insights into microbial diversity, biofilm formation, and carbohydrate degradation Liew, Kok Jun; Shahar, Saleha; Shamsir, Mohd Shahir figshare https://doi.org/10.6084/m9.figshare.25754948	dataset	January 2024

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