Draft genome sequences of strains CBS6241 and CBS6242 of the basidiomycetous yeast Filobasidium floriforme

Guerreiro, Marco Alexandre; Ahrendt, Steven; Pangilinan, Jasmyn; Chen, Cindy; Yan, Mi; Lipzen, Anna; Barry, Kerrie; Grigoriev, Igor V.; Begerow, Dominik; Nowrousian, Minou

doi:10.1093/g3journal/jkab398

Draft genome sequences of strains CBS6241 and CBS6242 of the basidiomycetous yeast Filobasidium floriforme

Journal Article · Mon Nov 15 00:00:00 EST 2021 · G3

DOI:https://doi.org/10.1093/g3journal/jkab398· OSTI ID:1894069

^[1]; Ahrendt, Steven ^[2]; Pangilinan, Jasmyn ^[2]; Chen, Cindy ^[2]; Yan, Mi ^[2]; Lipzen, Anna ^[2]; Barry, Kerrie ^[2]; Grigoriev, Igor V. ^[3]; Begerow, Dominik ^[1]; ^[1]

Ruhr Univ., Bochum (Germany)
USDOE Joint Genome Institute (JGI), Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
USDOE Joint Genome Institute (JGI), Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)

The Tremellomycetes are a species-rich group within the basidiomycete fungi; however, most analyses of this group to date have focused on pathogenic Cryptococcus species within the order Tremellales. Recent genome-assisted studies of other Tremellomycetes have identified interesting features with respect to biotechnological applications as well as the evolution of genes involved in mating and sexual development. Here, we report genome sequences of two strains of Filobasidium floriforme, a species from the order Filobasidiales, which branches basally to the Tremellales, Trichosporonales, and Holtermanniales. The assembled genomes of strains CBS6241 and CBS6242 are 27.4 Mb and 26.4 Mb in size, respectively, with 8314 and 7695 predicted protein-coding genes. Overall sequence identity at nucleic acid level between the strains is 97%. Among the predicted genes are pheromone precursor and pheromone receptor genes as well as two genes encoding homedomain (HD) transcription factors, which are predicted to be part of the mating type (MAT) locus. Sequence analysis indicates that CBS6241 and CBS6242 carry different alleles for both the pheromone/receptor genes as well as the HD transcription factors. Orthology inference identified 1482 orthogroups exclusively found in F. floriforme, some of which were involved in carbohydrate transport and metabolism. Subsequent CAZyme repertoire characterization identified 267 and 247 enzymes for CBS6241 and CBS6242, respectively, the second highest number of CAZymes among the analyzed Tremellomycete species. In addition, F. floriforme contains five CAZymes absent in other species and several plant-cell-wall degrading CAZymes with the highest copy number in Tremellomycota, indicating the biotechnological potential of this species.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: German Research Foundation (DFG); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1894069

Journal Information:: G3, Journal Name: G3 Journal Issue: 2 Vol. 12; ISSN 2160-1836

Publisher:: Genetics Society of AmericaCopyright Statement

Country of Publication:: United States

Language:: English

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Additional file 6: of Comparative genomics provides new insights into the diversity, physiology, and sexuality of the only industrially exploited tremellomycete: Phaffia rhodozyma Bellora, Nicolás; Moliné, Martín; David-Palma, Márcia Figshare-Supplementary information for journal article at DOI: 10.1186/s12864-016-3244-7, 1 zip file (1.39 MB) https://doi.org/10.6084/m9.figshare.c.3618773_d8.v1	dataset	November 2016

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59 BASIC BIOLOGICAL SCIENCES
CAZymes
Filobasidiales
Filobasidium floriforme
basidiomycete
mating-type locus

Draft genome sequences of strains CBS6241 and CBS6242 of the basidiomycetous yeast Filobasidium floriforme

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References (67)

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