Evolution of p-coumaroylated lignin in eudicots provides new tools for cell wall engineering

Mottiar, Yaseen; Smith, Rebecca A.; Karlen, Steven D.; Ralph, John; Mansfield, Shawn D.

doi:10.1111/nph.18518

Title: Evolution of p-coumaroylated lignin in eudicots provides new tools for cell wall engineering

Journal Article · Tue Oct 04 00:00:00 EDT 2022 · New Phytologist

DOI:https://doi.org/10.1111/nph.18518· OSTI ID:1893925

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Univ. of British Columbia, Vancouver, BC (Canada); Univ. of Wisconsin, Madison, WI (United States)
Univ. of Wisconsin, Madison, WI (United States)

Ester-linked p-coumarate (pCA) is a hallmark feature of the secondary cell walls in commelinid monocot plants. It has been shown that pCA groups arise during lignin polymerisation from the participation of monolignol conjugates assembled by p-coumaroyl-CoA:monolignol transferase (PMT) enzymes, members of the BAHD superfamily of acyltransferases. Herein we report that a eudicot species, kenaf (Hibiscus cannabinus), naturally contains p-coumaroylated lignin in the core tissues of the stems but not in the bast fibres. Moreover, we identified a novel acyltransferase, HcPMT, that shares less than 30% amino acid identity with known monocot PMT sequences. Recombinant HcPMT showed a preference in enzyme assays for p-coumaroyl-CoA and benzoyl-CoA as acyl donor substrates, and sinapyl alcohol as an acyl acceptor. Heterologous expression of HcPMT in hybrid poplar trees led to the incorporation of pCA in lignin but no improvement in the saccharification potential of the wood. This work illustrates the value in mining diverse plant taxa for new monolignol acyltransferases. Furthermore, the occurrence of pCA outside of monocot lineages may represent another example of convergent evolution in lignin structure. Furthermore, this discovery expands textbook views on cell wall biochemistry and provides a new molecular tool for engineering the lignin of biomass feedstock plants.

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Research Organization:: Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018409

OSTI ID:: 1893925

Journal Information:: New Phytologist, Vol. 237, Issue 1; ISSN 0028-646X

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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Title: Evolution of p-coumaroylated lignin in eudicots provides new tools for cell wall engineering

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