Ferredoxin-linked chloroplast enzymes. Progress report

Technical Report · Fri Dec 31 04:00:00 EST 1993

DOI:https://doi.org/10.2172/188580· OSTI ID:188580

This report summarizes research on ferredoxin:NADP{sup +} oxidoreductase and ferredoxin:thioredoxin reductase. One of the primary goals of the original proposal was to map the ferredoxin-binding sites on three soluble enzymes that are located in spinach chloroplasts and utilize ferredoxin as an electron donor:Ferredoxin:NADP{sup +} oxidoreductase (FNR); ferredoxin:thioredoxin reductase (FTR) and glutamate synthase. As the availability of amino acid sequences for the enzymes are important in such studies, it was proposed that the amino acid sequence of glutamate synthase be determined. The amino acid sequences of FNR, FTR and ferredoxin are already known. An aim related to elucidating the binding sites on these enzymes for ferredoxin was to determine whether there is a common site on ferredoxin involved in binding to all of these ferredoxin-dependent chloroplast enzymes and, if so, to map it. One additional aim was to characterize thioredoxin binding by FTR and determine whether the same site on FTR is involved in binding both ferredoxin and thioredoxin. Considerable progress has been made on most of these original projects, although work conducted on FTR is still in its preliminary stages.

• Research Organization: Texas Tech Univ., Lubbock, TX (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: FG05-90ER20017
• OSTI ID: 188580
• Report Number(s): DOE/ER/20017--T2; ON: DE96004777
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
AMINO ACID SEQUENCE
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
FERREDOXIN
NADP
OXIDOREDUCTASES
PROGRESS REPORT
RECEPTORS