Biochemical and biophysical characterization of the major outer surface protein, OSP-A from North American and European isolates of Borrelia burgdorferi

Conference · Sun Dec 31 04:00:00 EST 1995

OSTI ID:188506

McGrath, B C; Dunn, J J ^[1]; France, L L ^[2]; Jaing, W; Polin, D; Gorgone, G; Luft, B; Dykhuizen, D ^[3]

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1. Brookhaven National Lab., Upton, NY (United States)
2. Plum Island Animal Disease Center, Greenport, NY (United States)
3. SUNY at Stony Brook, Stony Brook, NY (United States)

Lyme borreliosis, caused by the spirochete Borrelia burgdorferi, is the most common vector-borne disease in North America and Western Europe. As the major delayed immune response in humans, a better understanding of the major outer surface lipoproteins OspA and OspB are of much interest. These proteins have been shown to exhibit three distinct phylogenetic genotypes based on their DNA sequences. This paper describes the cloning of genomic DNA for each variant and amplification of PCR. DNA sequence data was used to derive computer driven phylogenetic analysis and deduced amino acid sequences. Overproduction of variant OspAs was carried out in E. coli using a T7-based expression system. Circular dichroism and fluorescence studies was carried out on the recombinant B31 PspA yielding evidence supporting a B31 protein containing 11% alpha-helix, 34% antiparallel beta-sheet, 12% parallel beta sheet.

• Research Organization: Brookhaven National Lab., Upton, NY (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: AC02-76CH00016
• OSTI ID: 188506
• Report Number(s): BNL--62484; CONF-9509283--1; ON: DE96004157
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
AMINO ACID SEQUENCE
ANTIGENS
BACTERIAL DISEASES
BIOLOGICAL VARIABILITY
DESIGN
DNA-CLONING
ESCHERICHIA COLI
GENE REGULATION
GENETIC ENGINEERING
MEMBRANE PROTEINS
PROTEIN STRUCTURE
SPIROCHAETES
VACCINES