The roles of photochemical and non-photochemical quenching in regulating photosynthesis depend on the phases of fluctuating light conditions

Han, Jimei; Gu, Lianhong; Warren, Jeffrey M.; Guha, Anirban; Mclennan, David A.; Zhang, Wangfeng; Zhang, Yali

doi:10.1093/treephys/tpab133

The roles of photochemical and non-photochemical quenching in regulating photosynthesis depend on the phases of fluctuating light conditions

Journal Article · Thu Oct 07 00:00:00 EDT 2021 · Tree Physiology (Online)

DOI:https://doi.org/10.1093/treephys/tpab133· OSTI ID:1883669

Han, Jimei ^[1]; ^[2]; ^[2]; ^[2]; Mclennan, David A. ^[2]; Zhang, Wangfeng ^[3]; ^[3]

Shihezi University (China); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Cornell University, Ithaca, NY (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Shihezi University (China)

We report the induction and relaxation of photochemistry and non-photochemical quenching (NPQ) are not instantaneous and require time to respond to fluctuating environments. There is a lack of integrated understanding on how photochemistry and NPQ influence photosynthesis in fluctuating environments. We measured the induction and relaxation of chlorophyll a fluorescence and gas exchange in poplar and cotton at varying temperatures under saturating and fluctuating lights. When the light shifted from dark to high, the fraction of open reaction centers in photosystem II (q_L) gradually increased while NPQ increased suddenly and then remained stable. Temperature significantly changed the response of q_L but not that of NPQ during the dark to high light transition. Increased q_L led to higher photosynthesis but their precise relationship was affected by NPQ and temperature. q_L was significantly related to biochemical capacity. Thus, q_L appears to be a strong indicator of the activation of carboxylase, leading to the similar dynamics between q_L and photosynthesis. When the light shifted from high to low intensity, NPQ is still engaged at a high level, causing a stronger decline in photosynthesis. Our finding suggests that the dynamic effects of photochemistry and NPQ on photosynthesis depend on the phases of environmental fluctuations and interactive effects of light and temperature. Across the full spectra of light fluctuation, the slow induction of q_L is a more important limiting factor than the slow relaxation of NPQ for photosynthesis in typical ranges of temperature for photosynthesis. The findings provided a new perspective to improve photosynthetic productivity with molecular biology under natural fluctuating environments.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: China Scholarship Council (CSC); National Natural Science Foundation of China; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1883669

Alternate ID(s):: OSTI ID: 2538518

Journal Information:: Tree Physiology (Online), Journal Name: Tree Physiology (Online) Journal Issue: 4 Vol. 42; ISSN 1758-4469

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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