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Curcumin differentially affects cell cycle and cell death in acute and chronic myeloid leukemia cells

Journal Article · · Oncology Letters
DOI:https://doi.org/10.3892/ol.2018.8112· OSTI ID:1880958
 [1];  [1];  [1];  [2];  [3];  [4];  [5];  [5]
  1. Center of Studies and Advance Research, Mexico City (Mexico)
  2. National Institute Polytechnique, Mexico City (Mexico)
  3. National Autonomous University of Mexico (UNAM), Mexico City (Mexico); Hospital General, Mexico City (Mexico)
  4. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Univ. of California, Davis, CA (United States)
  5. National Institute of Genomic Medicine, Mexico City (Mexico)
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Curcumin is a phytochemical with potent antineoplastic properties. The antitumoral effects of curcumin in cells derived from chronic or acute myeloid leukemia have been already described. However, a comparative study of the cytostatic and cytotoxic effects of curcumin on chronic and acute myeloid leukemia cells has not yet been performed. In the present study, the cellular effects of curcumin on cell lines derived from chronic or acute myeloid leukemia were examined. Dose and time-response assays were performed with curcumin on HL-60 and K562 cells. Cell viability was evaluated with trypan blue exclusion test and cell death by flow cytometry using a fluorescent molecular probe. Additionally, a cell cycle profile was analyzed, and protein markers of cell cycle progression and cell death were investigated. In the present study, the K562 cells showed a higher sensitivity to the cytostatic and cytotoxic effects of curcumin compared with HL-60. In addition, curcumin induced G1 phase arrest in HL-60 cells and G2/M phase arrest in K562 cells. Furthermore, curcumin-related cell death in HL-60 was associated with the processed forms of caspases-9 and -3 proteins, whereas in K562 cells, both the processed and the unprocessed forms were present. Accordingly, activity of these caspases was significantly higher in HL-60 cells compared with that in K562. In conclusion, curcumin elicits different cellular mechanisms in chronic or acute myeloid leukemia cells and the powerful antitumoral effect was more potent in K562 compared with HL-60 cells.
Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Organization:
Consejo Nacional de Ciencia y Tecnología (CONACyT); USDOE National Nuclear Security Administration (NNSA)
Grant/Contract Number:
AC52-07NA27344
OSTI ID:
1880958
Report Number(s):
LLNL-JRNL-778997; 973039
Journal Information:
Oncology Letters, Journal Name: Oncology Letters Journal Issue: 5 Vol. 15; ISSN 1792-1074
Publisher:
Spandidos PublicationsCopyright Statement
Country of Publication:
United States
Language:
English

Cited By (2)

Synergistic Cytotoxicity of Methyl 4-Hydroxycinnamate and Carnosic Acid to Acute Myeloid Leukemia Cells via Calcium-Dependent Apoptosis Induction journal May 2019
Evaluation of some genes and proteins involved in apoptosis on human chronic myeloid leukemia cells (K562 cells) by datura innoxia leaves aqueous extract text January 2019

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Related Subjects

60 APPLIED LIFE SCIENCES
HL-60
K562
cell cycle
apoptosis
chemoprevention
curcumin
leukemia