Determination of fluorine distribution in shark teeth by laser-induced breakdown spectroscopy

Manard, Benjamin T.; Hintz, Christopher J.; Quarles, C. Derrick; Burns, William; Zirakparvar, N. Alex; Dunlap, Daniel R.; Beiswenger, Toya; Cruz-Uribe, Alicia M.; Petrus, Joseph A.; Hexel, Cole R.

doi:10.1093/mtomcs/mfac050

Determination of fluorine distribution in shark teeth by laser-induced breakdown spectroscopy

Journal Article · Tue Jul 05 00:00:00 EDT 2022 · Metallomics (Online)

DOI:https://doi.org/10.1093/mtomcs/mfac050· OSTI ID:1878687

^[1]; Hintz, Christopher J. ^[2]; Quarles, C. Derrick ^[3]; Burns, William ^[2]; ^[1]; Dunlap, Daniel R. ^[1]; Beiswenger, Toya ^[1]; ^[4]; Petrus, Joseph A. ^[5]; ^[1]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Savannah State University, Savannah, GA (United States). Marine and Environmental Sciences
Elemental Scientific, Inc., Omaha, NE (United States)
Univ. of Maine, Orono, ME (United States)
Elemental Scientific Lasers, Bozeman, MT (United States)

Quantifying the chemical composition of fast-growing hard tissues in the environment can shed valuable information in terms of understanding ecosystems both prehistoric and current. Changes in chemical composition can be correlated with environmental conditions and can provide information about the organism's life. Sharks can lose 0.1 to 1.1 teeth/day, depending on species, which offers a unique opportunity to record environmental changes over a short duration of time. Shark teeth contain a biomineral phase that is made up of fluorapatite [Ca₅(PO₄)₃F], and the F distribution within the tooth can be correlated to tooth hardness. Typically, this is determined by bulk acid digestion, energy-dispersive X-ray spectroscopy (EDS), or wavelength-dispersive spectroscopy. Here we present laser-induced breakdown spectroscopy (LIBS) as an alternative and faster approach for determining F distribution within shark teeth. Using a two-volume laser ablation chamber (TwoVol3) with innovative embedded collection optics for LIBS, shark teeth were investigated from sand tiger (Carcharias Taurus), tiger (Galeocerdo Cuvier), and hammerhead sharks (Sphyrnidae). Fluorine distribution was mapped using the CaF 603 nm band (CaF, Β ²Σ⁺ → X ²Σ⁺) and quantified using apatite reference materials. In addition, F measurements were cross referenced with EDS analyses to validate the findings. Distributions of F (603 nm), Na (589 nm), and H (656 nm) within the tooth correlate well with the expected biomineral composition and expected tooth hardness. This rapid methodology could transform the current means of determining F distribution, particularly when large sample specimens (350 mm², presented here) and large quantities of specimens are of interest.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1878687

Journal Information:: Metallomics (Online), Journal Name: Metallomics (Online) Journal Issue: 7 Vol. 14; ISSN 1756-591X

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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36 MATERIALS SCIENCE
fluorine
laser-induced breakdown spectroscopy (LIBS)
mapping
shark teeth

Determination of fluorine distribution in shark teeth by laser-induced breakdown spectroscopy

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