Engineering Curcumin Biosynthesis in Poplar Affects Lignification and Biomass Yield

De Meester, Barbara; Oyarce, Paula; Vanholme, Ruben; Van Acker, Rebecca; Tsuji, Yukiko; Vangeel, Thijs; Van den Bosch, Sander; Van Doorsselaere, Jan; Sels, Bert; Ralph, John; Boerjan, Wout

doi:10.3389/fpls.2022.943349

Engineering Curcumin Biosynthesis in Poplar Affects Lignification and Biomass Yield

Journal Article · Mon Jul 04 04:00:00 EDT 2022 · Frontiers in Plant Science

DOI:https://doi.org/10.3389/fpls.2022.943349· OSTI ID:1876007

De Meester, Barbara ^[1]; Oyarce, Paula ^[2]; Vanholme, Ruben ^[2]; Van Acker, Rebecca ^[2]; Tsuji, Yukiko ^[3]; Vangeel, Thijs ^[4]; Van den Bosch, Sander ^[4]; Van Doorsselaere, Jan ^[5]; Sels, Bert ^[4]; Ralph, John ^[3]; Boerjan, Wout ^[2]

Ghent Univ. (Belgium); VIB Center for Plant Systems Biology, Ghent (Belgium); Ghent University, Department of Plant Biotechnology and Bioinformatics
Ghent Univ. (Belgium); VIB Center for Plant Systems Biology, Ghent (Belgium)
Univ. of Wisconsin, Madison, WI (United States); Great Lakes Bioenergy Research Center, Madison, WI (United States)
KU Leuven (Belgium)
VIVES, Roeselare (Belgium)

Lignocellulosic biomass is recalcitrant toward deconstruction into simple sugars mainly due to the presence of lignin. By engineering plants to partially replace traditional lignin monomers with alternative ones, lignin degradability and extractability can be enhanced. Previously, the alternative monomer curcumin has been successfully produced and incorporated into lignified cell walls of Arabidopsis by the heterologous expression of DIKETIDE-CoA SYNTHASE (DCS) and CURCUMIN SYNTHASE2 (CURS2). The resulting transgenic plants did not suffer from yield penalties and had an increased saccharification yield after alkaline pretreatment. Here, we translated this strategy into the bio-energy crop poplar. Via the heterologous expression of DCS and CURS2 under the control of the secondary cell wall CELLULOSE SYNTHASE A8-B promoter (ProCesA8-B), curcumin was also produced and incorporated into the lignified cell walls of poplar. ProCesA8-B:DCS_CURS2 transgenic poplars, however, suffered from shoot-tip necrosis and yield penalties. Compared to that of the wild-type (WT), the wood of transgenic poplars had 21% less cellulose, 28% more matrix polysaccharides, 23% more lignin and a significantly altered lignin composition. More specifically, ProCesA8-B:DCS_CURS2 lignin had a reduced syringyl/guaiacyl unit (S/G) ratio, an increased frequency of p-hydroxyphenyl (H) units, a decreased frequency of p-hydroxybenzoates and a higher fraction of phenylcoumaran units. Without, or with alkaline or hot water pretreatment, the saccharification efficiency of the transgenic lines was equal to that of the WT. These differences in (growth) phenotype illustrate that translational research in crops is essential to assess the value of an engineering strategy for applications. Further fine-tuning of this research strategy (e.g., by using more specific promoters or by translating this strategy to other crops such as maize) might lead to transgenic bio-energy crops with cell walls more amenable to deconstruction without settling in yield.

View Accepted Manuscript (DOE)

Research Organization:: Great Lakes Bioenergy Research Center, Madison, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); Ghent University

Grant/Contract Number:: SC0018409

OSTI ID:: 1876007

Journal Information:: Frontiers in Plant Science, Journal Name: Frontiers in Plant Science Vol. 13; ISSN 1664-462X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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