Mass spectral imaging showing the plant growth-promoting rhizobacteria's effect on the Brachypodium awn

Zhang, Yuchen; Komorek, Rachel; Zhu, Zihua; Huang, Qiaoyun; Chen, Wenli; Jansson, Janet; Jansson, Christer; Yu, Xiao-Ying

doi:10.1116/6.0001949

Title: Mass spectral imaging showing the plant growth-promoting rhizobacteria's effect on the Brachypodium awn

Journal Article · Sun May 01 00:00:00 EDT 2022 · Biointerphases

DOI:https://doi.org/10.1116/6.0001949· OSTI ID:1873810

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Huazhong Univ. of Science and Technology, Wuhan (China)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

The plant growth-promoting rhizobacteria (PGPR) on the host plant surface play a key role in biological control and pathogenic response in plant functions and growth. However, it is difficult to elucidate the PGPR effect on plants. Such information is important in biomass production and conversion. Brachypodium distachyon (Brachypodium), a genomics model for bioenergy and native grasses, was selected as a C3 plant model; and the Gram-negative Pseudomonas fluorescens SBW25 ( P.) and Gram-positive Arthrobacter chlorophenolicus A6 ( A.) were chosen as representative PGPR strains. The PGPRs were introduced to the Brachypodium seed's awn prior to germination, and their possible effects on the seeding and growth were studied using different modes of time-of-flight secondary ion mass spectrometry (ToF-SIMS) measurements, including a high mass-resolution spectral collection and delayed image extraction. We observed key plant metabolic products and biomarkers, such as flavonoids, phenolic compounds, fatty acids, and auxin indole-3-acetic acid in the Brachypodium awns. Furthermore, principal component analysis and two-dimensional imaging analysis reveal that the Brachypodium awns are sensitive to the PGPR, leading to chemical composition and morphology changes on the awn surface. Our results show that ToF-SIMS can be an effective tool to probe cell-to-cell interactions at the biointerface. This work provides a new approach to studying the PGPR effects on awn and shows its potential for the research of plant growth in the future.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725; 32092565; 90001; 50093; AC05-76RL01830

OSTI ID:: 1873810

Alternate ID(s):: OSTI ID: 1873569; OSTI ID: 1886276

Report Number(s):: PNNL-SA-168695; TRN: US2307106

Journal Information:: Biointerphases, Vol. 17, Issue 3; ISSN 1934-8630

Publisher:: American Institute of Physics (AIP)Copyright Statement

Country of Publication:: United States

Language:: English

References (71)

Flow cytometric and microscopic analysis of GFP-tagged Pseudomonas fluorescens bacteria Tombolini, Riccardo; Unge, Annika; Davey, Mary Ellen FEMS Microbiology Ecology, Vol. 22, Issue 1 https://doi.org/10.1111/j.1574-6941.1997.tb00352.x	journal	January 1997
Phenolic acids act as signaling molecules in plant-microbe symbioses Mandal, Santi M.; Chakraborty, Dipjyoti; Dey, Satyahari Plant Signaling & Behavior, Vol. 5, Issue 4 https://doi.org/10.4161/psb.5.4.10871	journal	April 2010
Climate-smart crops with enhanced photosynthesis Jansson, Christer; Vogel, John; Hazen, Samuel Journal of Experimental Botany, Vol. 69, Issue 16 https://doi.org/10.1093/jxb/ery213	journal	May 2018
Potential Effects of Awn Length Variation on Seed Yield and Components, Seed Dispersal and Germination Performance in Siberian Wildrye (Elymus sibiricus L.) Ntakirutimana, Fabrice; Xiao, Bowen; Xie, Wengang Plants, Vol. 8, Issue 12 https://doi.org/10.3390/plants8120561	journal	December 2019
Brachypodium distachyon . A New Model System for Functional Genomics in Grasses Draper, John; Mur, Luis A. J.; Jenkins, Glyn Plant Physiology, Vol. 127, Issue 4 https://doi.org/10.1104/pp.010196	journal	December 2001
Brachypodium: a promising hub between model species and cereals Girin, Thomas; David, Laure C.; Chardin, Camille Journal of Experimental Botany, Vol. 65, Issue 19 https://doi.org/10.1093/jxb/eru376	journal	September 2014
Characterisation of biological material with ToF-SIMS: a review Fearn, S. Materials Science and Technology, Vol. 31, Issue 2 https://doi.org/10.1179/1743284714Y.0000000668	journal	March 2014
Imaging of wood tissue by ToF-SIMS: Critical evaluation and development of sample preparation techniques Tokareva, E. N.; Fardim, P.; Pranovich, A. V. Applied Surface Science, Vol. 253, Issue 18 https://doi.org/10.1016/j.apsusc.2007.03.059	journal	July 2007
Biological tissue sample preparation for time-of-flight secondary ion mass spectrometry (ToF–SIMS) imaging Yoon, Sohee; Lee, Tae Geol Nano Convergence, Vol. 5, Issue 1 https://doi.org/10.1186/s40580-018-0157-y	journal	September 2018
In Situ Molecular Imaging of the Biofilm and Its Matrix Ding, Yuanzhao; Zhou, Yufan; Yao, Juan Analytical Chemistry, Vol. 88, Issue 22 https://doi.org/10.1021/acs.analchem.6b03909	journal	October 2016
Mass Spectrometry Imaging of Specialized Metabolites for Predicting Lichen Fitness and Snail Foraging Gadea, Alice; Fanuel, Mathieu; Le Lamer, Anne-Cécile Plants, Vol. 9, Issue 1 https://doi.org/10.3390/plants9010070	journal	January 2020
A concise review of mass spectrometry imaging Amstalden van Hove, Erika R.; Smith, Donald F.; Heeren, Ron M. A. Journal of Chromatography A, Vol. 1217, Issue 25 https://doi.org/10.1016/j.chroma.2010.01.033	journal	June 2010
Metabolic Imaging at the Single-Cell Scale: Recent Advances in Mass Spectrometry Imaging Gilmore, Ian S.; Heiles, Sven; Pieterse, Cornelius L. Annual Review of Analytical Chemistry, Vol. 12, Issue 1 https://doi.org/10.1146/annurev-anchem-061318-115516	journal	June 2019
Preserved and variable spatial‐chemical changes of lipids across tomato leaves in response to central vein wounding reveals potential origin of linolenic acid in signal transduction cascade Veličković, Dušan; Chu, Rosalie K.; Henkel, Corinna Plant-Environment Interactions, Vol. 2, Issue 1 https://doi.org/10.1002/pei3.10038	journal	February 2021
Biological activity of five antibacterial flavonoids from Combretum erythrophyllum (Combretaceae) Martini, N. D.; Katerere, D. R. P.; Eloff, J. N. Journal of Ethnopharmacology, Vol. 93, Issue 2-3 https://doi.org/10.1016/j.jep.2004.02.030	journal	August 2004
Localization of Flavonoids in Seeds by Cluster Time-of-Flight Secondary Ion Mass Spectrometry Imaging Seyer, Alexandre; Einhorn, Jacques; Brunelle, Alain Analytical Chemistry, Vol. 82, Issue 6 https://doi.org/10.1021/ac902528t	journal	March 2010
The cryo-TOF-SIMS/SEM system for the analysis of the chemical distribution in freeze-fixed Cryptomeria japonica wood : Cryo-TOF-SIMS/SEM system for analysis of the freeze-fixed wood Kuroda, Katsushi; Fujiwara, Takeshi; Imai, Takanori Surface and Interface Analysis, Vol. 45, Issue 1 https://doi.org/10.1002/sia.4979	journal	April 2012
TOF-SIMS: Accurate mass scale calibration Green, F. M.; Gilmore, I. S.; Seah, M. P. Journal of the American Society for Mass Spectrometry, Vol. 17, Issue 4 https://doi.org/10.1016/j.jasms.2005.12.005	journal	April 2006
Isolation of Plant Hormone (Indole-3-Acetic Acid - IAA) Producing Rhizobacteria and Study on Their Effects on Maize Seedling Lwin, Khin Mya; Myint, Moe Moe; Tar, Tar Engineering Journal, Vol. 16, Issue 5 https://doi.org/10.4186/ej.2012.16.5.137	journal	October 2012
Visualizing the distribution of strawberry plant metabolites at different maturity stages by MALDI-TOF imaging mass spectrometry Wang, Jin; Yang, Ethan; Chaurand, Pierre Food Chemistry, Vol. 345 https://doi.org/10.1016/j.foodchem.2020.128838	journal	May 2021
Surface evolution of synthetic bilgewater emulsion Son, Jiyoung; Shen, Yanjie; Yao, Jenn Chemosphere, Vol. 236 https://doi.org/10.1016/j.chemosphere.2019.124345	journal	December 2019
Intracellular and extracellular PGPR: commonalities and distinctions in the plant–bacterium signaling processes Gray, E. J.; Smith, D. L. Soil Biology and Biochemistry, Vol. 37, Issue 3 https://doi.org/10.1016/j.soilbio.2004.08.030	journal	March 2005
Plant growth-promoting bacteria in the rhizo- and endosphere of plants: Their role, colonization, mechanisms involved and prospects for utilization Compant, Stéphane; Clément, Christophe; Sessitsch, Angela Soil Biology and Biochemistry, Vol. 42, Issue 5 https://doi.org/10.1016/j.soilbio.2009.11.024	journal	May 2010
Understanding the Molecular Signatures in Leaves and Flowers by Desorption Electrospray Ionization Mass Spectrometry (DESI MS) Imaging Hemalatha, R. G.; Pradeep, T. Journal of Agricultural and Food Chemistry, Vol. 61, Issue 31 https://doi.org/10.1021/jf4011998	journal	July 2013
Future Cereal Starch Bioengineering: Cereal Ancestors Encounter Gene Technology and Designer Enzymes Blennow, Andreas; Jensen, Susanne L.; Shaik, Shahnoor S. Cereal Chemistry Journal, Vol. 90, Issue 4 https://doi.org/10.1094/CCHEM-01-13-0010-FI	journal	July 2013
Delayed extraction for improved resolution of ion/surface collision products by time-of-flight mass spectrometry Haney, Lisa L.; Riederer, Donald E. Analytica Chimica Acta, Vol. 397, Issue 1-3 https://doi.org/10.1016/S0003-2670(99)00407-9	journal	October 1999
Toward Mass Spectrometry Imaging in the Metabolomics Scale: Increasing Metabolic Coverage Through Multiple On-Tissue Chemical Modifications Dueñas, Maria Emilia; Larson, Evan A.; Lee, Young Jin Frontiers in Plant Science, Vol. 10 https://doi.org/10.3389/fpls.2019.00860	journal	July 2019
Plant hormones and seed germination Miransari, Mohammad; Smith, D. L. Environmental and Experimental Botany, Vol. 99 https://doi.org/10.1016/j.envexpbot.2013.11.005	journal	March 2014
Ambient molecular imaging by laser ablation electrospray ionization mass spectrometry with ion mobility separation Li, Hang; Smith, Brian K.; Márk, László International Journal of Mass Spectrometry, Vol. 377 https://doi.org/10.1016/j.ijms.2014.06.025	journal	February 2015
Antibacterial and allelopathic activity of extract from Castanea sativa leaves Basile, A.; Sorbo, S.; Giordano, S. Fitoterapia, Vol. 71 https://doi.org/10.1016/S0367-326X(00)00185-4	journal	August 2000
Mass Spectrometry in Plant-omics Gemperline, Erin; Keller, Caitlin; Li, Lingjun Analytical Chemistry, Vol. 88, Issue 7 https://doi.org/10.1021/acs.analchem.5b02938	journal	March 2016
Characterization of syntrophic Geobacter communities using ToF-SIMS Wei, Wenchao; Zhang, Yanyan; Komorek, Rachel Biointerphases, Vol. 12, Issue 5 https://doi.org/10.1116/1.4986832	journal	December 2017
Arthrobacter chlorophenolicus sp. nov., a new species capable of degrading high concentrations of 4-chlorophenol Westerberg, K.; Elvang, A. M.; Stackebrandt, E. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 50, Issue 6 https://doi.org/10.1099/00207713-50-6-2083	journal	November 2000
Alleviation of salt stress by plant growth regulators and IAA producing bacteria in wheat Egamberdieva, Dilfuza Acta Physiologiae Plantarum, Vol. 31, Issue 4 https://doi.org/10.1007/s11738-009-0297-0	journal	March 2009
Surface Characterization of Dilute Acid Pretreated Populus deltoides by ToF-SIMS Jung, Seokwon; Foston, Marcus; Sullards, M. Cameron Energy & Fuels, Vol. 24, Issue 2 https://doi.org/10.1021/ef901062p	journal	February 2010
Analysis of Chemotherapeutic Drug Delivery at the Single Cell Level Using 3D-MSI-TOF-SIMS Vanbellingen, Quentin P.; Castellanos, Anthony; Rodriguez-Silva, Monica Journal of The American Society for Mass Spectrometry, Vol. 27, Issue 12 https://doi.org/10.1007/s13361-016-1485-y	journal	August 2016
Brachypodium : A Monocot Grass Model Genus for Plant Biology Scholthof, Karen-Beth G.; Irigoyen, Sonia; Catalan, Pilar The Plant Cell, Vol. 30, Issue 8 https://doi.org/10.1105/tpc.18.00083	journal	July 2018
Improved mass resolution and mass accuracy in TOF-SIMS spectra and images using argon gas cluster ion beams Shon, Hyun Kyong; Yoon, Sohee; Moon, Jeong Hee Biointerphases, Vol. 11, Issue 2 https://doi.org/10.1116/1.4941447	journal	June 2016
Mass spectrometry imaging for plant biology: a review Boughton, Berin A.; Thinagaran, Dinaiz; Sarabia, Daniel Phytochemistry Reviews, Vol. 15, Issue 3 https://doi.org/10.1007/s11101-015-9440-2	journal	October 2015
Information from complexity: Challenges of TOF-SIMS data interpretation Graham, Daniel J.; Wagner, Matthew S.; Castner, David G. Applied Surface Science, Vol. 252, Issue 19 https://doi.org/10.1016/j.apsusc.2006.02.149	journal	July 2006
TRANSPARENT TESTA8 Inhibits Seed Fatty Acid Accumulation by Targeting Several Seed Development Regulators in Arabidopsis Chen, Mingxun; Xuan, Lijie; Wang, Zhong Plant Physiology, Vol. 165, Issue 2 https://doi.org/10.1104/pp.114.235507	journal	April 2014
All fatty acids are not equal: discrimination in plant membrane lipids Millar, Anthony A.; Smith, Mark A.; Kunst, Ljerka Trends in Plant Science, Vol. 5, Issue 3 https://doi.org/10.1016/S1360-1385(00)01566-1	journal	March 2000
Rhizosphere engineering: Enhancing sustainable plant ecosystem productivity Ahkami, Amir H.; Allen White, Richard; Handakumbura, Pubudu P. Rhizosphere, Vol. 3 https://doi.org/10.1016/j.rhisph.2017.04.012	journal	June 2017
Organic matrix-free imaging mass spectrometry Kim, Eunjin; Kim, Jisu; Choi, Inseong BMB Reports, Vol. 53, Issue 7 https://doi.org/10.5483/BMBRep.2020.53.7.078	journal	July 2020
Identification of auxin from Zea coleoptile tips by mass spectrometry Greenwood, M. S.; Shaw, S.; Hillman, J. R. Planta, Vol. 108, Issue 2 https://doi.org/10.1007/BF00386080	journal	January 1972
Use of mass spectrometry for imaging metabolites in plants: Mass spectrometry for imaging metabolites in plants Lee, Young Jin; Perdian, David C.; Song, Zhihong The Plant Journal, Vol. 70, Issue 1 https://doi.org/10.1111/j.1365-313X.2012.04899.x	journal	March 2012
Imaging Matrix Assisted Laser Desorption Ionization Mass Spectrometry: a technique to map plant metabolites within tissues at high spatial resolution Burrell, Mm; Earnshaw, Cj; Clench, Mr Journal of Experimental Botany, Vol. 58, Issue 4 https://doi.org/10.1093/jxb/erl139	journal	September 2006
Two-dimensional and three-dimensional dynamic imaging of live biofilms in a microchannel by time-of-flight secondary ion mass spectrometry Hua, Xin; Marshall, Matthew J.; Xiong, Yijia Biomicrofluidics, Vol. 9, Issue 3 https://doi.org/10.1063/1.4919807	journal	May 2015
Effect of Different Plant Growth Promoting Rhizobacteria on Maize Seed Germination and Seedling Development Noumavo, Pacôme A.; Kochoni, Eméric; Didagbé, Yédéou O. American Journal of Plant Sciences, Vol. 04, Issue 05 https://doi.org/10.4236/ajps.2013.45125	journal	January 2013
Latest applications of 3D ToF-SIMS bio-imaging Fletcher, John S. Biointerphases, Vol. 10, Issue 1 https://doi.org/10.1116/1.4907727	journal	March 2015
Imaging liquids using microfluidic cells Yu, Xiao-Ying; Liu, Bingwen; Yang, Li Microfluidics and Nanofluidics, Vol. 15, Issue 6 https://doi.org/10.1007/s10404-013-1199-4	journal	May 2013
Seed Germination Inhibitors: Molecular and Phytochemical Aspects Samajdar, Saptarshi; Mukherjee, Souvik; Das, Partha Pratim International Journal of Applied Pharmaceutical Sciences and Research, Vol. 3, Issue 02 https://doi.org/10.21477/ijapsr.v3i02.11365	journal	March 2018
The potential of mass spectrometry imaging in plant metabolomics: a review Heyman, Heino M.; Dubery, Ian A. Phytochemistry Reviews, Vol. 15, Issue 2 https://doi.org/10.1007/s11101-015-9416-2	journal	June 2015
Time-of-flight secondary ion mass spectrometry imaging of biological samples with delayed extraction for high mass and high spatial resolutions: TOF-SIMS imaging of biological samples with delayed extraction Vanbellingen, Quentin P.; Elie, Nicolas; Eller, Michael J. Rapid Communications in Mass Spectrometry, Vol. 29, Issue 13 https://doi.org/10.1002/rcm.7210	journal	May 2015
Lipid imaging with time-of-flight secondary ion mass spectrometry (ToF-SIMS) Passarelli, Melissa K.; Winograd, Nicholas Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol. 1811, Issue 11 https://doi.org/10.1016/j.bbalip.2011.05.007	journal	November 2011
Mass spectrometry imaging as a potential technique for diagnostic of Huanglongbing disease using fast and simple sample preparation de Moraes Pontes, João Guilherme; Vendramini, Pedro Henrique; Fernandes, Laura Soler Scientific Reports, Vol. 10, Issue 1 https://doi.org/10.1038/s41598-020-70385-4	journal	August 2020
High resolution imaging and 3D analysis of Ag nanoparticles in cells with ToF-SIMS and delayed extraction Henss, Anja; Otto, Svenja-K.; Schaepe, Kaija Biointerphases, Vol. 13, Issue 3 https://doi.org/10.1116/1.5015957	journal	June 2018
Improving the Molecular Ion Signal Intensity for In Situ Liquid SIMS Analysis Zhou, Yufan; Yao, Juan; Ding, Yuanzhao Journal of The American Society for Mass Spectrometry, Vol. 27, Issue 12 https://doi.org/10.1007/s13361-016-1478-x	journal	September 2016
Legume seed flavonoids and nitrogenous metabolites as signals and protectants in early seedling development Ndakidemi, Patrick A.; Dakora, Felix D. Functional Plant Biology, Vol. 30, Issue 7 https://doi.org/10.1071/FP03042	journal	January 2003
ToF-SIMS characterization of glyoxal surface oxidation products by hydrogen peroxide: A comparison between dry and liquid samples: SIMS characterization of glyoxal surface oxidation by hydrogen peroxide Sui, Xiao; Zhou, Yufan; Zhang, Fei Surface and Interface Analysis, Vol. 50, Issue 10 https://doi.org/10.1002/sia.6334	journal	November 2017
What more can TOF-SIMS bring than other MS imaging methods? Touboul, David; Brunelle, Alain Bioanalysis, Vol. 8, Issue 5 https://doi.org/10.4155/bio.16.11	journal	March 2016
Awnedness affects grain yield and kernel weight in near-isogenic lines of durum wheat Motzo, Rosella; Giunta, Francesco Australian Journal of Agricultural Research, Vol. 53, Issue 12 https://doi.org/10.1071/AR02008	journal	January 2002
Antibacterial Activity of Some Flavonoids and Organic Acids Widely Distributed in Plants Adamczak, Artur; Ożarowski, Marcin; Karpiński, Tomasz M. Journal of Clinical Medicine, Vol. 9, Issue 1 https://doi.org/10.3390/jcm9010109	journal	December 2019
Impact of PGPR inoculation on growth and antioxidant status of wheat under saline conditions: Effect of rhizobacteria on wheat in saline conditions Upadhyay, Sudhir Kumar; Singh, Jay Shankar; Saxena, Anil Kumar Plant Biology, Vol. 14, Issue 4 https://doi.org/10.1111/j.1438-8677.2011.00533.x	journal	December 2011
A concise tutorial review of TOF-SIMS based molecular and cellular imaging Massonnet, Philippe; Heeren, Ron M. A. Journal of Analytical Atomic Spectrometry, Vol. 34, Issue 11 https://doi.org/10.1039/C9JA00164F	journal	January 2019
Correlative surface imaging reveals chemical signatures for bacterial hotspots on plant roots Liu, Wen; Huang, Liuqin; Komorek, Rachel The Analyst, Vol. 145, Issue 2 https://doi.org/10.1039/C9AN01954E	journal	January 2020
Biomarker imaging of single diatom cells in a microbial mat using time-of-flight secondary ion mass spectrometry (ToF-SIMS) Leefmann, Tim; Heim, Christine; Kryvenda, Anastasiia Organic Geochemistry, Vol. 57 https://doi.org/10.1016/j.orggeochem.2013.01.005	journal	April 2013
Brachypodium distachyon and Setaria viridis : Model Genetic Systems for the Grasses Brutnell, Thomas P.; Bennetzen, Jeffrey L.; Vogel, John P. Annual Review of Plant Biology, Vol. 66, Issue 1 https://doi.org/10.1146/annurev-arplant-042811-105528	journal	April 2015
Sample Preparation of Corn Seed Tissue to Prevent Analyte Relocations for Mass Spectrometry Imaging Kim, Shin Hye; Kim, Jeongkwon; Lee, Young Jin Journal of the American Society for Mass Spectrometry, Vol. 28, Issue 8 https://doi.org/10.1007/s13361-017-1682-3	journal	May 2017
Molecular imaging of plant–microbe interactions on the Brachypodium seed surface Zhang, Yuchen; Komorek, Rachel; Son, Jiyoung The Analyst, Vol. 146, Issue 19 https://doi.org/10.1039/D1AN00205H	journal	January 2021
A SHORT INTERNODES (SHI) family transcription factor gene regulates awn elongation and pistil morphology in barley Yuo, Takahisa; Yamashita, Yuko; Kanamori, Hiroyuki Journal of Experimental Botany, Vol. 63, Issue 14 https://doi.org/10.1093/jxb/ers182	journal	July 2012

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59 BASIC BIOLOGICAL SCIENCES
Plant Growth-Promoting Rhizobacteria
Brachypodium distachyon
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metabolite
awn
growth potential
Plant Growth-Promoting Rhizobacteria
Brachypodium distachyon
ToF-SIMS
metabolite
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growth potential

Title: Mass spectral imaging showing the plant growth-promoting rhizobacteria's effect on the Brachypodium awn

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