Knocking Out the Wall: Revised Protocols for Gene Targeting in Physcomitrella patens

Roberts, Alison W.; Dimos, Christos S.; Budziszek, Jr., Michael J.; Goss, Chessa A.; Lai, Virginia; Chaves, Arielle M.

doi:10.1007/978-1-0716-0621-6_8

Knocking Out the Wall: Revised Protocols for Gene Targeting in Physcomitrella patens

Journal Article · Fri Jul 03 00:00:00 EDT 2020 · Methods in Molecular Biology

DOI:https://doi.org/10.1007/978-1-0716-0621-6_8· OSTI ID:1873749

^[1]; Dimos, Christos S. ^[2]; Budziszek, Jr., Michael J. ^[2]; Goss, Chessa A. ^[2]; Lai, Virginia ^[2]; Chaves, Arielle M. ^[2]

Univ. of Rhode Island, Kingston, RI (United States); University of Rhode Island
Univ. of Rhode Island, Kingston, RI (United States)

The moss Physcomitrella patens has become established as a model for investigating plant gene function due to the feasibility of gene targeting. The chemical composition of the P. patens cell wall is similar to that of vascular plants and phylogenetic analyses of glycosyltransferase sequences from the P. patens genome have identified genes that putatively encode cell wall biosynthetic enzymes, providing a basis for investigating the evolution of cell wall polysaccharides and the enzymes that synthesize them. Here, the protocols described in this chapter provide methods for targeted gene knockout in P. patens, from constructing vectors and maintaining cultures to transforming protoplasts and analysing the genotypes and phenotypes of the resulting transformed lines.

View Accepted Manuscript (DOE)

Research Organization:: Pennsylvania State Univ., University Park, PA (United States)

Sponsoring Organization:: National Research Initiative Competitive; USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0001090

OSTI ID:: 1873749

Journal Information:: Methods in Molecular Biology, Journal Name: Methods in Molecular Biology Vol. 2149; ISSN 1064-3745

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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