Total Internal Reflection of Two Lasers in a Single Planar Optical Waveguide

Kocheril, Philip Abraham; Lenz, Kiersten Danielle; Mukundan, Harshini

doi:10.1149/2754-2726/ac6523

Total Internal Reflection of Two Lasers in a Single Planar Optical Waveguide

Journal Article · Tue May 03 00:00:00 EDT 2022 · ECS Sensors Plus

DOI:https://doi.org/10.1149/2754-2726/ac6523· OSTI ID:1869626

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Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

Multiplex biomarker quantitation is ideal for tracking the progression of a disease, but sensitive, specific, and quantitative multiplex biosensing remains challenging. We achieved total internal reflection with two lasers in a single planar optical waveguide by coupling 532 nm laser light into the diffraction grating of a waveguide and 635 nm laser light directly into the thin film of the same waveguide. We confirmed that the evanescent fields generated in the waveguide can excite two fluorescent dyes (Alexa Fluor 532 and Alexa Fluor 647) held to the surface of the waveguide through biotin-streptavidin chemistry. This sensing concept holds significant potential as a platform for multiplex biomarker detection.

View Accepted Manuscript (DOE)

Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: U.S. Department of Defense (DOD); USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: 89233218CNA000001

OSTI ID:: 1869626

Report Number(s):: LA-UR-22-21004

Journal Information:: ECS Sensors Plus, Journal Name: ECS Sensors Plus Journal Issue: 2 Vol. 1; ISSN 2754-2726

Publisher:: IOP ScienceCopyright Statement

Country of Publication:: United States

Language:: English

References (34)

Portable chemiluminescence multiplex biosensor for quantitative detection of three B19 DNA genotypes Mirasoli, Mara; Bonvicini, Francesca; Dolci, Luisa Stella Analytical and Bioanalytical Chemistry, Vol. 405, Issue 2-3 https://doi.org/10.1007/s00216-012-6573-7	journal	November 2012
Chapter 7 Total Internal Reflection Fluorescence Microscopy Axelrod, Daniel Methods in Cell Biology https://doi.org/10.1016/S0091-679X(08)00607-9	book	December 2008
Fluorescence based fiber optic and planar waveguide biosensors. A review Benito-Peña, Elena; Valdés, Mayra Granda; Glahn-Martínez, Bettina Analytica Chimica Acta, Vol. 943 https://doi.org/10.1016/j.aca.2016.08.049	journal	November 2016
A nanoplasmonic biosensor for label-free multiplex detection of cancer biomarkers Lee, Jong Uk; Nguyen, Anh H.; Sim, Sang Jun Biosensors and Bioelectronics, Vol. 74 https://doi.org/10.1016/j.bios.2015.06.059	journal	December 2015
A shape-code nanoplasmonic biosensor for multiplex detection of Alzheimer's disease biomarkers Kim, Hanbi; Lee, Jong Uk; Song, Sojin Biosensors and Bioelectronics, Vol. 101 https://doi.org/10.1016/j.bios.2017.10.018	journal	March 2018
Highly sensitive optoelectrical biosensor for multiplex allergy diagnosis Mas, Salvador; Badran, Ahmed A.; Juárez, María-José Biosensors and Bioelectronics, Vol. 166 https://doi.org/10.1016/j.bios.2020.112438	journal	October 2020
Current approaches and challenges for the metabolite profiling of complex natural extracts Wolfender, Jean-Luc; Marti, Guillaume; Thomas, Aurélien Journal of Chromatography A, Vol. 1382 https://doi.org/10.1016/j.chroma.2014.10.091	journal	February 2015
Surface plasmon resonance imaging based multiplex biosensor: Integration of biomolecular screening, detection and kinetics estimation Krishnamoorthy, Ganeshram; Carlen, Edwin T.; van den Berg, Albert Sensors and Actuators B: Chemical, Vol. 148, Issue 2 https://doi.org/10.1016/j.snb.2010.04.015	journal	July 2010
A compact and multichannel optical biosensor based on a wavelength interrogated input grating coupler Grego, Sonia; Gilchrist, Kristin H.; Carlson, James B. Sensors and Actuators B: Chemical, Vol. 161, Issue 1 https://doi.org/10.1016/j.snb.2011.11.020	journal	January 2012
An array fluorescent biosensor based on planar waveguide for multi-analyte determination in water samples Liu, Lanhua; Zhou, Xiaohong; Lu, Mifang Sensors and Actuators B: Chemical, Vol. 240 https://doi.org/10.1016/j.snb.2016.08.118	journal	March 2017
A portable automated multianalyte biosensor Golden, J.; Taitt, C.; Shriverlake, L. Talanta, Vol. 65, Issue 5 https://doi.org/10.1016/j.talanta.2004.03.072	journal	March 2005
Multiplex Biosensor Using Gold Nanorods Yu, Chenxu; Irudayaraj, Joseph Analytical Chemistry, Vol. 79, Issue 2 https://doi.org/10.1021/ac061730d	journal	January 2007
Plasmonic Properties of the Multispot Copper-Capped Nanoparticle Array Chip and Its Application to Optical Biosensors for Pathogen Detection of Multiplex DNAs Kim, Do-Kyun; Yoo, Seung Min; Park, Tae Jung Analytical Chemistry, Vol. 83, Issue 16 https://doi.org/10.1021/ac2007762	journal	July 2011
Quantitative Multiplex Detection of Pathogen Biomarkers on Multichannel Waveguides Mukundan, Harshini; Xie, Hongzhi; Price, Dominique Analytical Chemistry, Vol. 82, Issue 1 https://doi.org/10.1021/ac901497g	journal	January 2010
Total Internal Reflection Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy Otosu, Takuhiro; Yamaguchi, Shoichi The Journal of Physical Chemistry B, Vol. 122, Issue 22 https://doi.org/10.1021/acs.jpcb.8b01176	journal	May 2018
Real-Time Monitoring of Azo Dye Interfacial Adsorption at Silica–Water Interface by Total Internal Reflection-Induced Surface Evanescent Wave Xiong, Yan; Wang, Qing; Duan, Ming Langmuir, Vol. 34, Issue 26 https://doi.org/10.1021/acs.langmuir.8b00722	journal	June 2018
Multiplex Serum Cytokine Immunoassay Using Nanoplasmonic Biosensor Microarrays Chen, Pengyu; Chung, Meng Ting; McHugh, Walker ACS Nano, Vol. 9, Issue 4 https://doi.org/10.1021/acsnano.5b00396	journal	March 2015
Total Internal Reflection Fluorescence Microscopy in Cell Biology: Total Internal Reflection Fluorescence Axelrod, Daniel Traffic, Vol. 2, Issue 11 https://doi.org/10.1034/j.1600-0854.2001.21104.x	journal	November 2001
Bring on the biomarkers Poste, George Nature, Vol. 469, Issue 7329 https://doi.org/10.1038/469156a	journal	January 2011
Total internal reflection with fluorescence correlation spectroscopy Thompson, Nancy L.; Steele, Bridgett L. Nature Protocols, Vol. 2, Issue 4 https://doi.org/10.1038/nprot.2007.110	journal	April 2007
Integrated optical waveguide-based fluorescent immunosensor for fast and sensitive detection of microcystin-LR in lakes: Optimization and Analysis Liu, Lanhua; Zhou, Xiaohong; Wilkinson, James S. Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/s41598-017-03939-8	journal	June 2017
Direct detection of bacteremia by exploiting host-pathogen interactions of lipoteichoic acid and lipopolysaccharide Kubicek-Sutherland, Jessica Z.; Vu, Dung M.; Noormohamed, Aneesa Scientific Reports, Vol. 9, Issue 1 https://doi.org/10.1038/s41598-019-42502-5	journal	April 2019
Accurate single-pair Förster resonant energy transfer through combination of pulsed interleaved excitation, time correlated single-photon counting, and fluorescence correlation spectroscopy Rüttinger, Steffen; Macdonald, Rainer; Krämer, Benedikt Journal of Biomedical Optics, Vol. 11, Issue 2 https://doi.org/10.1117/1.2187425	journal	January 2006
Multiplexed label-free optical biosensor for medical diagnostics Bottazzi, Barbara; Fornasari, Lucia; Frangolho, Ana Journal of Biomedical Optics, Vol. 19, Issue 1 https://doi.org/10.1117/1.JBO.19.1.017006	journal	January 2014
New astroglial injury-defined biomarkers for neurotrauma assessment Halford, Julia; Shen, Sean; Itamura, Kyohei Journal of Cerebral Blood Flow & Metabolism, Vol. 37, Issue 10 https://doi.org/10.1177/0271678X17724681	journal	August 2017
A Multiplex Electrochemical Biosensor for Bloodstream Infection Diagnosis Gao, Jian; Jeffries, Lindsie; Mach, Kathleen E. SLAS Technology, Vol. 22, Issue 4 https://doi.org/10.1177/2211068216651232	journal	August 2017
Blood Biomarkers for Traumatic Brain Injury: A Quantitative Assessment of Diagnostic and Prognostic Accuracy Gan, Zoe S.; Stein, Sherman C.; Swanson, Randel Frontiers in Neurology, Vol. 10 https://doi.org/10.3389/fneur.2019.00446	journal	April 2019
Optical Biosensor Platforms Display Varying Sensitivity for the Direct Detection of Influenza RNA Courtney, Samantha; Stromberg, Zachary; Myers y. Gutiérrez, Adán Biosensors, Vol. 11, Issue 10 https://doi.org/10.3390/bios11100367	journal	September 2021
Portable Waveguide-Based Optical Biosensor Kocheril, Philip A.; Lenz, Kiersten D.; Mascareñas, David D. L. Biosensors, Vol. 12, Issue 4 https://doi.org/10.3390/bios12040195	journal	March 2022
Proteomic Challenges: Sample Preparation Techniques for Microgram-Quantity Protein Analysis from Biological Samples Feist, Peter; Hummon, Amanda International Journal of Molecular Sciences, Vol. 16, Issue 2 https://doi.org/10.3390/ijms16023537	journal	February 2015
Pediatric Tuberculosis: The Impact of “Omics” on Diagnostics Development Jakhar, Shailja; Bitzer, Alexis A.; Stromberg, Loreen R. International Journal of Molecular Sciences, Vol. 21, Issue 19 https://doi.org/10.3390/ijms21196979	journal	September 2020
Waveguide-Based Biosensors for Pathogen Detection Mukundan, Harshini; Anderson, Aaron; Grace, W. Kevin Sensors, Vol. 9, Issue 7, p. 5783-5809 https://doi.org/10.3390/s90705783	journal	July 2009
Influenza Hemagglutinin and Neuraminidase: Yin–Yang Proteins Coevolving to Thwart Immunity Kosik, Ivan; Yewdell, Jonathan W. Viruses, Vol. 11, Issue 4 https://doi.org/10.3390/v11040346	journal	April 2019
Proteomics: Challenges, Techniques and Possibilities to Overcome Biological Sample Complexity Chandramouli, Kondethimmanahalli; Qian, Pei-Yuan Human Genomics and Proteomics, Vol. 1, Issue 1 https://doi.org/10.4061/2009/239204	journal	January 2009

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