Bioactive scaffolds with enhanced supramolecular motion promote recovery from spinal cord injury

Álvarez, Z.; Kolberg-Edelbrock, A. N.; Sasselli, I. R.; Ortega, J. A.; Qiu, R.; Syrgiannis, Z.; Mirau, P. A.; Chen, F.; Chin, S. M.; Weigand, S.; Kiskinis, E.; Stupp, S. I.

doi:10.1126/science.abh3602

Bioactive scaffolds with enhanced supramolecular motion promote recovery from spinal cord injury

Journal Article · Thu Nov 11 00:00:00 EST 2021 · Science

DOI:https://doi.org/10.1126/science.abh3602· OSTI ID:1861056

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Northwestern Univ., Chicago, IL (United States); CBES
Northwestern Univ., Chicago, IL (United States); Northwestern Univ., Evanston, IL (United States)
Northwestern Univ., Chicago, IL (United States); Northwestern Univ., Evanston, IL (United States); Basque Research and Technology Alliance (BRTA), San Sebastian (Spain)
Northwestern Univ., Chicago, IL (United States)
Air Force Research Laboratories, Wright-Patterson AFB, OH (United States)
Northwestern Univ., DND-CAT, Argonne, IL (United States)

The signaling of cells by scaffolds of synthetic molecules that mimic proteins is known to be effective in the regeneration of tissues. Here, we describe peptide amphiphile supramolecular polymers containing two distinct signals and test them in a mouse model of severe spinal cord injury. One signal activates the transmembrane receptor β1-integrin and a second one activates the basic fibroblast growth factor 2 receptor. By mutating the peptide sequence of the amphiphilic monomers in nonbioactive domains, we intensified the motions of molecules within scaffold fibrils. This resulted in notable differences in vascular growth, axonal regeneration, myelination, survival of motor neurons, reduced gliosis, and functional recovery. Here, we hypothesize that the signaling of cells by ensembles of molecules could be optimized by tuning their internal motions.

View Accepted Manuscript (DOE)

Research Organization:: Northwestern Univ., Evanston, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0000989

OSTI ID:: 1861056

Alternate ID(s):: OSTI ID: 1831228
OSTI ID: 1832599

Journal Information:: Science, Journal Name: Science Journal Issue: 6569 Vol. 374; ISSN 0036-8075

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: English

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