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Title: Genotype-Specific Neutralization of Norovirus Is Mediated by Antibodies Against the Protruding Domain of the Major Capsid Protein

Journal Article · · Journal of Infectious Diseases
 [1];  [2];  [1];  [1]; ORCiD logo [1]
  1. Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA
  2. Center for Food Safety and Applied Nutrition, Food and Drug Administration, Laurel, Maryland, USA
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Abstract Human noroviruses are the most common viral agents of acute gastroenteritis. Recently, human intestinal enteroids were shown to be permissive for norovirus infection. We tested their suitability as a system to study norovirus neutralization. Hyperimmune sera raised against virus-like particles (VLPs) representing different genotypes showed highly specific neutralization activity against GII.4 and GII.6 noroviruses. Carbohydrate blocking assays and neutralization exhibited similar patterns in antibody responses. Notably, sera produced against chimeric VLPs that presented swapped structural shell and protruding (P) domains, from different genotypes showed that neutralization is primarily mediated by antibodies mapping to the P domain of the norovirus capsid protein. This study provides empirical information on the antigenic differences among genotypes as measured by neutralization, which could guide vaccine design.

Sponsoring Organization:
USDOE
OSTI ID:
1860506
Journal Information:
Journal of Infectious Diseases, Journal Name: Journal of Infectious Diseases Vol. 225 Journal Issue: 7; ISSN 0022-1899
Publisher:
Oxford University PressCopyright Statement
Country of Publication:
United States
Language:
English

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