Identification of four genes coding for isoforms of murinoglobulin, the monomeric mouse {alpha}{sub 2}-macroglobulin: Characterization of the exons coding for the bait region
- Univ. of Leuven (Belgium); and others
Murinoglobulins are the single chain members of the {alpha}{sub 2}-macroglobulin family of proteinase inhibitors in the mouse. DNA clones representing the genes coding for four different murinoglobulins were isolated from three independent mouse genomic DNA libraries. Sequence analysis demonstrated that in each gene two exons are coding for the bait region. This is the specific protein sequence in each {alpha}-macroglobulin, which is functionally important since it is extremely sensitive to cleavage by different proteinases. The molecular data established the existence of at least four different murinoglobulin genes. Three of these corresponded to the three cDNA clones previously identified. Sequencing of intron-exon boundaries and intron sizing allowed us to construct physical maps of the region from exon 15 to exon 25. These data indicate that the mouse presents a genetic repertoire of the {alpha}{sub 2}-macroglobulin family much more complex than originally anticipated.
- OSTI ID:
- 186047
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 22; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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