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Volumetric micro-injector for capillary electrophoresis

Patent ·
OSTI ID:1860210
; ;
A volumetric microfluidic injector for capillary electrophoresis (CE) for highly repeatable sample injection has been designed and built to eliminate known injection bias in hydrodynamic injection. A defined volume from 1-10 nL or 0.1-100 nL of sample is confined in a defined region of a micro-valve PDMS microfluidic injector chip and electrophoretic potential is applied to drive sample into a separation device such as an embedded fused silica capillary for separation and detection. Using a 75 μm ID capillary, the RSD of an absorbance peak area as low as 1.32% (n=11) is obtained. As a comparison, the time-dependent injection was tested using the same chip which resulted in an inferior repeatability.
Research Organization:
University of California, Oakland, CA (United States)
Sponsoring Organization:
USDOE; National Institute of Health (NIH)
DOE Contract Number:
SC0001249
Assignee:
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA (Oakland, CA)
Patent Number(s):
11,209,390
Application Number:
16/339,348
OSTI ID:
1860210
Country of Publication:
United States
Language:
English

References (11)

Electrokinetic sample preconcentration and hydrodynamic sample injection for microchip electrophoresis using a pneumatic microvalve: Microfluidics and Miniaturization journal November 2015
Instrumentation design for hydrodynamic sample injection in microchip electrophoresis: A review: Microfluidics and Miniaturization journal September 2012
Sample introduction techniques for microchip electrophoresis: A review journal May 2012
Design and Characterization of Poly(dimethylsiloxane)-Based Valves for Interfacing Continuous-Flow Sampling to Microchip Electrophoresis journal February 2006
How to increase precision in capillary electrophoresis journal January 2001
Development of fully automated quantitative capillary electrophoresis with high accuracy and repeatability: Development of fully automated quantitative capillary electrophoresis journal September 2015
Hydrodynamic injection with pneumatic valving for microchip electrophoresis with total analyte utilization journal April 2011
Pneumatic Microvalve-Based Hydrodynamic Sample Injection for High-Throughput, Quantitative Zone Electrophoresis in Capillaries journal June 2014
Integration of on-chip peristaltic pumps and injection valves with microchip electrophoresis and electrochemical detection journal July 2010
Why not using capillary electrophoresis in drug analysis? journal June 2006
Analytical instrument qualification in capillary electrophoresis: CE and CEC journal June 2012

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