Visualizing the gas channel of a monofunctional carbon monoxide dehydrogenase

Biester, Alison; Dementin, Sébastien; Drennan, Catherine L.

doi:10.1016/j.jinorgbio.2022.111774

Visualizing the gas channel of a monofunctional carbon monoxide dehydrogenase

Journal Article · Wed Feb 23 00:00:00 EST 2022 · Journal of Inorganic Biochemistry

DOI:https://doi.org/10.1016/j.jinorgbio.2022.111774· OSTI ID:1856382

Biester, Alison ^[1]; Dementin, Sébastien ^[2]; Drennan, Catherine L. ^[3]

Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
Aix-Marseille Univ. (France)
Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Canadian Inst. for Advanced Research, Ontario (Canada)

Carbon monoxide dehydrogenase (CODH) plays an important role in the processing of the one–carbon gases carbon monoxide and carbon dioxide. In CODH enzymes, these gases are channeled to and from the Ni-Fe-S active sites using hydrophobic cavities. In this work, we investigate these gas channels in a monofunctional CODH from Desulfovibrio vulgaris, which is unusual among CODHs for its oxygen-tolerance. By pressurizing D. vulgaris CODH protein crystals with xenon and solving the structure to 2.10 Å resolution, we identify 12 xenon sites per CODH monomer, thereby elucidating hydrophobic gas channels. We find that D. vulgaris CODH has one gas channel that has not been experimentally validated previously in a CODH, and a second channel that is shared with Moorella thermoacetica carbon monoxide dehydrogenase/acetyl-CoA synthase (CODH/ACS). This experimental visualization of D. vulgaris CODH gas channels lays groundwork for further exploration of factors contributing to oxygen-tolerance in this CODH, as well as study of channels in other CODHs. We dedicate this publication to the memory of Dick Holm, whose early studies of the Ni-Fe-S clusters of CODH inspired us all.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities Division

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1856382

Alternate ID(s):: OSTI ID: 1861871

Journal Information:: Journal of Inorganic Biochemistry, Journal Name: Journal of Inorganic Biochemistry Vol. 230; ISSN 0162-0134

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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