Structural basis for HCMV Pentamer recognition by neuropilin 2 and neutralizing antibodies

Wrapp, Daniel; Ye, Xiaohua; Ku, Zhiqiang; Su, Hang; Jones, Harrison G.; Wang, Nianshuang; Mishra, Akaash K.; Freed, Daniel C.; Li, Fengsheng; Tang, Aimin; Li, Leike; Jaijyan, Dabbu Kumar; Zhu, Hua; Wang, Dai; Fu, Tong-Ming; Zhang, Ningyan; An, Zhiqiang; McLellan, Jason S.

doi:10.1126/sciadv.abm2546

Structural basis for HCMV Pentamer recognition by neuropilin 2 and neutralizing antibodies

Journal Article · Fri Mar 11 04:00:00 EST 2022 · Science Advances

DOI:https://doi.org/10.1126/sciadv.abm2546· OSTI ID:1856359

^[1]; ^[2]; Ku, Zhiqiang ^[2]; Su, Hang ^[2]; Jones, Harrison G. ^[1]; Wang, Nianshuang ^[1]; ^[1]; Freed, Daniel C. ^[3]; Li, Fengsheng ^[3]; Tang, Aimin ^[3]; Li, Leike ^[2]; ^[4]; ^[4]; ^[3]; ^[2]; ^[2]; ^[2]; ^[1]

Univ. of Texas, Austin, TX (United States)
University of Texas Health Science Center, Houston, TX (United States)
Merck & Co. Inc., Kenilworth, NJ (United States)
Rutgers New Jersey Medical School, Newark, NJ (United States)

Human cytomegalovirus (HCMV) encodes multiple surface glycoprotein complexes to infect a variety of cell types. The HCMV Pentamer, composed of gH, gL, UL128, UL130, and UL131A, enhances entry into epithelial, endothelial, and myeloid cells by interacting with the cell surface receptor neuropilin 2 (NRP2). Despite the critical nature of this interaction, the molecular determinants that govern NRP2 recognition remain unclear. Here, we describe the cryo-EM structure of NRP2 bound to Pentamer. The high-affinity interaction between these proteins is calcium dependent and differs from the canonical carboxyl-terminal arginine (CendR) binding that NRP2 typically uses. We also determine the structures of four neutralizing human antibodies bound to the HCMV Pentamer to define susceptible epitopes. Two of these antibodies compete with NRP2 binding, but the two most potent antibodies recognize a previously unidentified epitope that does not overlap the NRP2-binding site. Collectively, these findings provide a structural basis for HCMV tropism and antibody-mediated neutralization.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1856359

Journal Information:: Science Advances, Journal Name: Science Advances Journal Issue: 10 Vol. 8; ISSN 2375-2548

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

References (84)

UCSF Chimera?A visualization system for exploratory research and analysis Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C. Journal of Computational Chemistry, Vol. 25, Issue 13 https://doi.org/10.1002/jcc.20084	journal	January 2004
Review of cytomegalovirus seroprevalence and demographic characteristics associated with infection: CMV seroprevalence Cannon, Michael J.; Schmid, D. Scott; Hyde, Terri B. Reviews in Medical Virology, Vol. 20, Issue 4 https://doi.org/10.1002/rmv.655	journal	June 2010
Molecular Phylogeny and Evolutionary Timescale for the Family of Mammalian Herpesviruses McGeoch, Duncan J.; Cook, Simon; Dolan, Aidan Journal of Molecular Biology, Vol. 247, Issue 3 https://doi.org/10.1006/jmbi.1995.0152	journal	March 1995
Leginon: An Automated System for Acquisition of Images from Vitreous Ice Specimens Carragher, Bridget; Kisseberth, Nick; Kriegman, David Journal of Structural Biology, Vol. 132, Issue 1 https://doi.org/10.1006/jsbi.2000.4314	journal	October 2000
The influence of extracellular and intracellular calcium on the secretion of renin Atchison, Douglas K.; Beierwaltes, William H. Pflügers Archiv - European Journal of Physiology, Vol. 465, Issue 1 https://doi.org/10.1007/s00424-012-1107-x	journal	April 2012
Specific cell surface labels in the visual centers of Xenopus laevis tadpole identified using monoclonal antibodies Takagi, Shin; Tsuji, Toshiaki; Amagai, Takashi Developmental Biology, Vol. 122, Issue 1 https://doi.org/10.1016/0012-1606(87)90335-6	journal	July 1987
The A5 antigen, a candidate for the neuronal recognition molecule, has homologies to complement components and coagulation factors Takagi, Shin; Hirata, Tatsumi; Agata, Kiyokazu Neuron, Vol. 7, Issue 2 https://doi.org/10.1016/0896-6273(91)90268-5	journal	August 1991
Neuropilin-1 Is Expressed by Endothelial and Tumor Cells as an Isoform-Specific Receptor for Vascular Endothelial Growth Factor Soker, Shay; Takashima, Seiji; Miao, Hua Quan Cell, Vol. 92, Issue 6 https://doi.org/10.1016/S0092-8674(00)81402-6	journal	March 1998
An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a Central Viral Receptor Martinez-Martin, Nadia; Marcandalli, Jessica; Huang, Christine S. Cell, Vol. 174, Issue 5 https://doi.org/10.1016/j.cell.2018.06.028	journal	August 2018
Structures of HCMV Trimer reveal the basis for receptor recognition and cell entry Kschonsak, Marc; Rougé, Lionel; Arthur, Christopher P. Cell, Vol. 184, Issue 5 https://doi.org/10.1016/j.cell.2021.01.036	journal	March 2021
Congenital cytomegalovirus infection following first trimester maternal infection: Symptoms at birth and outcome Pass, Robert F.; Fowler, Karen B.; Boppana, Suresh B. Journal of Clinical Virology, Vol. 35, Issue 2 https://doi.org/10.1016/j.jcv.2005.09.015	journal	February 2006
Structural Basis for VEGF-C Binding to Neuropilin-2 and Sequestration by a Soluble Splice Form Parker, Matthew W.; Linkugel, Andrew D.; Goel, Hira Lal Structure, Vol. 23, Issue 4 https://doi.org/10.1016/j.str.2015.01.018	journal	April 2015
Crystal Structure of the Neuropilin-1 MAM Domain: Completing the Neuropilin-1 Ectodomain Picture Yelland, Tamas; Djordjevic, Snezana Structure, Vol. 24, Issue 11 https://doi.org/10.1016/j.str.2016.08.017	journal	November 2016
A novel high-throughput neutralization assay for supporting clinical evaluations of human cytomegalovirus vaccines Tang, Aimin; Li, Fengsheng; Freed, Daniel C. Vaccine, Vol. 29, Issue 46 https://doi.org/10.1016/j.vaccine.2011.08.086	journal	October 2011
SerialEM: A Program for Automated Tilt Series Acquisition on Tecnai Microscopes Using Prediction of Specimen Position Mastronarde, David N. Microscopy and Microanalysis, Vol. 9, Issue S02 https://doi.org/10.1017/S1431927603445911	journal	July 2003
cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination Punjani, Ali; Rubinstein, John L.; Fleet, David J. Nature Methods, Vol. 14, Issue 3 https://doi.org/10.1038/nmeth.4169	journal	February 2017
Platelet-derived growth factor-α receptor is the cellular receptor for human cytomegalovirus gHgLgO trimer Kabanova, Anna; Marcandalli, Jessica; Zhou, Tongqing Nature Microbiology, Vol. 1, Issue 8 https://doi.org/10.1038/nmicrobiol.2016.82	journal	June 2016
Structural basis for receptor recognition by Lujo virus Cohen-Dvashi, Hadas; Kilimnik, Itay; Diskin, Ron Nature Microbiology, Vol. 3, Issue 10 https://doi.org/10.1038/s41564-018-0224-5	journal	August 2018
Real-time cryo-electron microscopy data preprocessing with Warp Tegunov, Dimitry; Cramer, Patrick Nature Methods, Vol. 16, Issue 11 https://doi.org/10.1038/s41592-019-0580-y	journal	October 2019
Non-uniform refinement: adaptive regularization improves single-particle cryo-EM reconstruction Punjani, Ali; Zhang, Haowei; Fleet, David J. Nature Methods, Vol. 17, Issue 12 https://doi.org/10.1038/s41592-020-00990-8	journal	November 2020
DeepEMhancer: a deep learning solution for cryo-EM volume post-processing Sanchez-Garcia, Ruben; Gomez-Blanco, Josue; Cuervo, Ana Communications Biology, Vol. 4, Issue 1 https://doi.org/10.1038/s42003-021-02399-1	journal	July 2021
Structural studies of neuropilin/antibody complexes provide insights into semaphorin and VEGF binding Appleton, Brent A.; Wu, Ping; Maloney, Janice The EMBO Journal, Vol. 26, Issue 23 https://doi.org/10.1038/sj.emboj.7601906	journal	November 2007
Human cytomegalovirus virion protein complex required for epithelial and endothelial cell tropism Wang, D.; Shenk, T. Proceedings of the National Academy of Sciences, Vol. 102, Issue 50 https://doi.org/10.1073/pnas.0509201102	journal	November 2005
Structural basis for ligand and heparin binding to neuropilin B domains Vander Kooi, C. W.; Jusino, M. A.; Perman, B. Proceedings of the National Academy of Sciences, Vol. 104, Issue 15 https://doi.org/10.1073/pnas.0700043104	journal	April 2007
C-end rule peptides mediate neuropilin-1-dependent cell, vascular, and tissue penetration Teesalu, Tambet; Sugahara, Kazuki N.; Kotamraju, Venkata Ramana Proceedings of the National Academy of Sciences, Vol. 106, Issue 38 https://doi.org/10.1073/pnas.0908201106	journal	September 2009
Pentameric complex of viral glycoprotein H is the primary target for potent neutralization by a human cytomegalovirus vaccine Freed, Daniel C.; Tang, Qi; Tang, Aimin Proceedings of the National Academy of Sciences, Vol. 110, Issue 51 https://doi.org/10.1073/pnas.1316517110	journal	December 2013
Mechanism for neutralizing activity by the anti-CMV gH/gL monoclonal antibody MSL-109 Fouts, A. E.; Comps-Agrar, L.; Stengel, K. F. Proceedings of the National Academy of Sciences, Vol. 111, Issue 22 https://doi.org/10.1073/pnas.1404653111	journal	May 2014
Structural and biochemical studies of HCMV gH/gL/gO and Pentamer reveal mutually exclusive cell entry complexes Ciferri, Claudio; Chandramouli, Sumana; Donnarumma, Danilo Proceedings of the National Academy of Sciences, Vol. 112, Issue 6 https://doi.org/10.1073/pnas.1424818112	journal	January 2015
The pentameric complex drives immunologically covert cell–cell transmission of wild-type human cytomegalovirus Murrell, Isa; Bedford, Carmen; Ladell, Kristin Proceedings of the National Academy of Sciences, Vol. 114, Issue 23 https://doi.org/10.1073/pnas.1704809114	journal	May 2017
HCMV trimer- and pentamer-specific antibodies synergize for virus neutralization but do not correlate with congenital transmission Vanarsdall, Adam L.; Chin, Andrea L.; Liu, Jing Proceedings of the National Academy of Sciences, Vol. 116, Issue 9 https://doi.org/10.1073/pnas.1814835116	journal	February 2019
OR14I1 is a receptor for the human cytomegalovirus pentameric complex and defines viral epithelial cell tropism E., Xiaofei; Meraner, Paul; Lu, Ping Proceedings of the National Academy of Sciences, Vol. 116, Issue 14 https://doi.org/10.1073/pnas.1814850116	journal	March 2019
Neuropilin-1-VEGFR-2 Complexing Requires the PDZ-binding Domain of Neuropilin-1 Prahst, Claudia; Héroult, Mélanie; Lanahan, Anthony A. Journal of Biological Chemistry, Vol. 283, Issue 37 https://doi.org/10.1074/jbc.C800137200	journal	September 2008
Structural Basis for Selective Vascular Endothelial Growth Factor-A (VEGF-A) Binding to Neuropilin-1* Parker, Matthew W.; Xu, Ping; Li, Xiaobo Journal of Biological Chemistry, Vol. 287, Issue 14 https://doi.org/10.1074/jbc.M111.331140	journal	March 2012
The human cytomegalovirus terminase complex as an antiviral target: a close-up view Ligat, G.; Cazal, R.; Hantz, S. FEMS Microbiology Reviews, Vol. 42, Issue 2 https://doi.org/10.1093/femsre/fuy004	journal	January 2018
Animal Models of Congenital Cytomegalovirus Transmission: Implications for Vaccine Development Roark, Hunter K.; Jenks, Jennifer A.; Permar, Sallie R. The Journal of Infectious Diseases, Vol. 221, Issue Supplement_1 https://doi.org/10.1093/infdis/jiz484	journal	March 2020
Fibroblasts, epithelial cells, endothelial cells and smooth muscle cells are major targets of human cytomegalovirus infection in lung and gastrointestinal tissues Sinzger, C.; Grefte, A.; Plachter, B. Journal of General Virology, Vol. 76, Issue 4 https://doi.org/10.1099/0022-1317-76-4-741	journal	April 1995
Sequential mutations associated with adaptation of human cytomegalovirus to growth in cell culture Dargan, D. J.; Douglas, E.; Cunningham, C. Journal of General Virology, Vol. 91, Issue 6 https://doi.org/10.1099/vir.0.018994-0	journal	May 2010
Genetic content of wild-type human cytomegalovirus Dolan, Aidan; Cunningham, Charles; Hector, Ralph D. Journal of General Virology, Vol. 85, Issue 5 https://doi.org/10.1099/vir.0.79888-0	journal	May 2004
Dendritic-cell infection by human cytomegalovirus is restricted to strains carrying functional UL131-128 genes and mediates efficient viral antigen presentation to CD8+ T cells Gerna, G. Journal of General Virology, Vol. 86, Issue 2 https://doi.org/10.1099/vir.0.80474-0	journal	February 2005
Role of human cytomegalovirus UL131A in cell type-specific virus entry and release Adler, Barbara; Scrivano, Laura; Ruzcics, Zsolt Journal of General Virology, Vol. 87, Issue 9 https://doi.org/10.1099/vir.0.81921-0	journal	September 2006
PHENIX : building new software for automated crystallographic structure determination Adams, Paul D.; Grosse-Kunstleve, Ralf W.; Hung, Li-Wei Acta Crystallographica Section D Biological Crystallography, Vol. 58, Issue 11 https://doi.org/10.1107/S0907444902016657	journal	October 2002
Coot model-building tools for molecular graphics Emsley, Paul; Cowtan, Kevin Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132 https://doi.org/10.1107/S0907444904019158	journal	November 2004
Solving structures of protein complexes by molecular replacement with Phaser McCoy, Airlie J. Acta Crystallographica Section D Biological Crystallography, Vol. 63, Issue 1 https://doi.org/10.1107/S0907444906045975	journal	December 2006
iMOSFLM : a new graphical interface for diffraction-image processing with MOSFLM Battye, T. Geoff G.; Kontogiannis, Luke; Johnson, Owen Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S0907444910048675	journal	March 2011
How good are my data and what is the resolution? Evans, Philip R.; Murshudov, Garib N. Acta Crystallographica Section D Biological Crystallography, Vol. 69, Issue 7 https://doi.org/10.1107/S0907444913000061	journal	June 2013
Iterative screen optimization maximizes the efficiency of macromolecular crystallization Jones, Harrison G.; Wrapp, Daniel; Gilman, Morgan S. A. Acta Crystallographica Section F Structural Biology Communications, Vol. 75, Issue 2 https://doi.org/10.1107/S2053230X18017338	journal	January 2019
ISOLDE : a physically realistic environment for model building into low-resolution electron-density maps Croll, Tristan Ian Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 6 https://doi.org/10.1107/S2059798318002425	journal	April 2018
Universal Prophylaxis or Preemptive Strategy for Cytomegalovirus Disease After Liver Transplantation: A Systematic Review and Meta-Analysis: Prophylaxis or Preemption for CMV Posttransplant Mumtaz, K.; Faisal, N.; Husain, S. American Journal of Transplantation, Vol. 15, Issue 2 https://doi.org/10.1111/ajt.13044	journal	December 2014
Structural studies of neuropilin-2 reveal a zinc ion binding site remote from the vascular endothelial growth factor binding pocket Tsai, Yi-Chun Isabella; Fotinou, Constantina; Rana, Rohini The FEBS Journal, Vol. 283, Issue 10 https://doi.org/10.1111/febs.13711	journal	April 2016
Neuropilin-1 is a host factor for SARS-CoV-2 infection Daly, James L.; Simonetti, Boris; Klein, Katja Science, Vol. 370, Issue 6518 https://doi.org/10.1126/science.abd3072	journal	November 2020
Structural basis for potent antibody-mediated neutralization of human cytomegalovirus Chandramouli, Sumana; Malito, Enrico; Nguyen, TuongVi Science Immunology, Vol. 2, Issue 12 https://doi.org/10.1126/sciimmunol.aan1457	journal	June 2017
In Vitro Characterization of Human Cytomegalovirus-Targeting Therapeutic Monoclonal Antibodies LJP538 and LJP539 Patel, Hetalkumar D.; Nikitin, Pavel; Gesner, Thomas Antimicrobial Agents and Chemotherapy, Vol. 60, Issue 8 https://doi.org/10.1128/AAC.00382-16	journal	August 2016
Potent Bispecific Neutralizing Antibody Targeting Glycoprotein B and the gH/gL/pUL128/130/131 Complex of Human Cytomegalovirus Su, Hang; Ye, Xiaohua; Freed, Daniel C. Antimicrobial Agents and Chemotherapy, Vol. 65, Issue 3 https://doi.org/10.1128/AAC.02422-20	journal	February 2021
Monoclonal Antibodies to Different Components of the Human Cytomegalovirus (HCMV) Pentamer gH/gL/pUL128L and Trimer gH/gL/gO as well as Antibodies Elicited during Primary HCMV Infection Prevent Epithelial Cell Syncytium Formation Gerna, Giuseppe; Percivalle, Elena; Perez, Laurent Journal of Virology, Vol. 90, Issue 14 https://doi.org/10.1128/JVI.00121-16	journal	July 2016
Antibodies against the gH/gL/UL128/UL130/UL131 Complex Comprise the Majority of the Anti-Cytomegalovirus (Anti-CMV) Neutralizing Antibody Response in CMV Hyperimmune Globulin Fouts, Ashley E.; Chan, Pamela; Stephan, Jean-Philippe Journal of Virology, Vol. 86, Issue 13 https://doi.org/10.1128/JVI.00467-12	journal	July 2012
Mutational Mapping of UL130 of Human Cytomegalovirus Defines Peptide Motifs within the C-Terminal Third as Essential for Endothelial Cell Infection Schuessler, Andrea; Sampaio, Kerstin Laib; Scrivano, Laura Journal of Virology, Vol. 84, Issue 18 https://doi.org/10.1128/JVI.00572-10	journal	September 2010
Charge Cluster-to-Alanine Scanning of UL128 for Fine Tuning of the Endothelial Cell Tropism of Human Cytomegalovirus Schuessler, Andrea; Sampaio, Kerstin Laib; Sinzger, Christian Journal of Virology, Vol. 82, Issue 22 https://doi.org/10.1128/JVI.01069-08	journal	November 2008
Human Cytomegalovirus Glycoprotein B Is Required for Virus Entry and Cell-to-Cell Spread but Not for Virion Attachment, Assembly, or Egress Isaacson, Marisa K.; Compton, Teresa Journal of Virology, Vol. 83, Issue 8 https://doi.org/10.1128/JVI.01251-08	journal	April 2009
Human Cytomegalovirus gH/gL/gO Promotes the Fusion Step of Entry into All Cell Types, whereas gH/gL/UL128-131 Broadens Virus Tropism through a Distinct Mechanism Zhou, Momei; Lanchy, Jean-Marc; Ryckman, Brent J. Journal of Virology, Vol. 89, Issue 17 https://doi.org/10.1128/JVI.01325-15	journal	September 2015
Impact of Sequence Variation in the UL128 Locus on Production of Human Cytomegalovirus in Fibroblast and Epithelial Cells Murrell, Isa; Tomasec, Peter; Wilkie, Gavin S. Journal of Virology, Vol. 87, Issue 19 https://doi.org/10.1128/JVI.01546-13	journal	October 2013
Vaccine-Derived Neutralizing Antibodies to the Human Cytomegalovirus gH/gL Pentamer Potently Block Primary Cytotrophoblast Infection Chiuppesi, Flavia; Wussow, Felix; Johnson, Erica Journal of Virology, Vol. 89, Issue 23 https://doi.org/10.1128/JVI.01701-15	journal	September 2015
Characterization of the Human Cytomegalovirus gH/gL/UL128-131 Complex That Mediates Entry into Epithelial and Endothelial Cells Ryckman, Brent J.; Rainish, Barb L.; Chase, Marie C. Journal of Virology, Vol. 82, Issue 1 https://doi.org/10.1128/JVI.01910-07	journal	January 2008
Neutralization of Diverse Human Cytomegalovirus Strains Conferred by Antibodies Targeting Viral gH/gL/pUL128-131 Pentameric Complex Ha, Sha; Li, Fengsheng; Troutman, Matthew C. Journal of Virology, Vol. 91, Issue 7 https://doi.org/10.1128/JVI.02033-16	journal	April 2017
Mutational Mapping of pUL131A of Human Cytomegalovirus Emphasizes Its Central Role for Endothelial Cell Tropism Schuessler, Andrea; Sampaio, Kerstin Laib; Straschewski, Sarah Journal of Virology, Vol. 86, Issue 1 https://doi.org/10.1128/JVI.05354-11	journal	January 2012
Human Cytomegalovirus Glycoprotein gO Complexes with gH/gL, Promoting Interference with Viral Entry into Human Fibroblasts but Not Entry into Epithelial Cells Vanarsdall, Adam L.; Chase, Marie C.; Johnson, David C. Journal of Virology, Vol. 85, Issue 22 https://doi.org/10.1128/JVI.05659-11	journal	November 2011
Human Cytomegalovirus UL131-128 Genes Are Indispensable for Virus Growth in Endothelial Cells and Virus Transfer to Leukocytes Hahn, G.; Revello, M. G.; Patrone, M. Journal of Virology, Vol. 78, Issue 18 https://doi.org/10.1128/JVI.78.18.10023-10033.2004	journal	August 2004
Human Cytomegalovirus (HCMV) Glycoprotein gB Promotes Virus Entry In Trans Acting as the Viral Fusion Protein Rather than as a Receptor-Binding Protein Wille, Paul T.; Wisner, Todd W.; Ryckman, Brent mBio, Vol. 4, Issue 3 https://doi.org/10.1128/mBio.00332-13	journal	July 2013
CD147 Promotes Entry of Pentamer-Expressing Human Cytomegalovirus into Epithelial and Endothelial Cells Vanarsdall, Adam L.; Pritchard, Sarah R.; Wisner, Todd W. mBio, Vol. 9, Issue 3 https://doi.org/10.1128/mBio.00781-18	journal	July 2018
Neuropilin-2-Mediated Tumor Growth and Angiogenesis in Pancreatic Adenocarcinoma Dallas, N. A.; Gray, M. J.; Xia, L. Clinical Cancer Research, Vol. 14, Issue 24 https://doi.org/10.1158/1078-0432.CCR-08-1520	journal	December 2008
Neuropilin-2 interacts with VEGFR-2 and VEGFR-3 and promotes human endothelial cell survival and migration Favier, Benoit; Alam, Antoine; Barron, Pauline Blood, Vol. 108, Issue 4 https://doi.org/10.1182/blood-2005-11-4447	journal	August 2006
Overexpression of a membrane protein, neuropilin, in chimeric mice causes anomalies in the cardiovascular system, nervous system and limbs Kitsukawa, T.; Shimono, A.; Kawakami, A. Development, Vol. 121, Issue 12 https://doi.org/10.1242/dev.121.12.4309	journal	December 1995
Fetal Human Cytomegalovirus Transmission Correlates with Delayed Maternal Antibodies to gH/gL/pUL128-130-131 Complex during Primary Infection Lilleri, Daniele; Kabanova, Anna; Revello, Maria Grazia PLoS ONE, Vol. 8, Issue 3 https://doi.org/10.1371/journal.pone.0059863	journal	March 2013
Antigenic Characterization of the HCMV gH/gL/gO and Pentamer Cell Entry Complexes Reveals Binding Sites for Potently Neutralizing Human Antibodies Ciferri, Claudio; Chandramouli, Sumana; Leitner, Alexander PLOS Pathogens, Vol. 11, Issue 10 https://doi.org/10.1371/journal.ppat.1005230	journal	October 2015
Structural Basis for Recognition of Human Enterovirus 71 by a Bivalent Broadly Neutralizing Monoclonal Antibody Ye, Xiaohua; Fan, Chen; Ku, Zhiqiang PLOS Pathogens, Vol. 12, Issue 3 https://doi.org/10.1371/journal.ppat.1005454	journal	March 2016
Human Cytomegalovirus gH/gL Forms a Stable Complex with the Fusion Protein gB in Virions Vanarsdall, Adam L.; Howard, Paul W.; Wisner, Todd W. PLOS Pathogens, Vol. 12, Issue 4 https://doi.org/10.1371/journal.ppat.1005564	journal	April 2016
Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-α as a key for entry Wu, Yiquan; Prager, Adrian; Boos, Simone PLOS Pathogens, Vol. 13, Issue 4 https://doi.org/10.1371/journal.ppat.1006281	journal	April 2017
Different functional states of fusion protein gB revealed on human cytomegalovirus by cryo electron tomography with Volta phase plate Si, Zhu; Zhang, Jiayan; Shivakoti, Sakar PLOS Pathogens, Vol. 14, Issue 12 https://doi.org/10.1371/journal.ppat.1007452	journal	December 2018
Potent neutralizing antibodies elicited by dengue vaccine in rhesus macaque target diverse epitopes Li, Leike; Meng, Weixu; Horton, Melanie PLOS Pathogens, Vol. 15, Issue 6 https://doi.org/10.1371/journal.ppat.1007716	journal	June 2019
Identification of adipocyte plasma membrane-associated protein as a novel modulator of human cytomegalovirus infection Ye, Xiaohua; Gui, Xun; Freed, Daniel C. PLOS Pathogens, Vol. 15, Issue 7 https://doi.org/10.1371/journal.ppat.1007914	journal	July 2019
Recognition of a highly conserved glycoprotein B epitope by a bivalent antibody neutralizing HCMV at a post-attachment step Ye, Xiaohua; Su, Hang; Wrapp, Daniel PLOS Pathogens, Vol. 16, Issue 8 https://doi.org/10.1371/journal.ppat.1008736	journal	August 2020
Active evolution of memory B-cells specific to viral gH/gL/pUL128/130/131 pentameric complex in healthy subjects with silent human cytomegalovirus infection Xia, Lin; Tang, Aimin; Meng, Weixu Oncotarget, Vol. 8, Issue 43 https://doi.org/10.18632/oncotarget.18359	journal	June 2017
Congenital Cytomegalovirus Infection: On the Relation between Type and Time of Maternal Infection and Infant's Symptoms Ahlfors, Karin; Forsgren, Marianne; Ivarsson, Sten-Anders Scandinavian Journal of Infectious Diseases, Vol. 15, Issue 2 https://doi.org/10.3109/inf.1983.15.issue-2.01	journal	June 1983
Neutralizing Monoclonal Antibodies Reduce Human Cytomegalovirus Infection and Spread in Developing Placentas Tabata, Takako; Petitt, Matthew; Fang-Hoover, June Vaccines, Vol. 7, Issue 4 https://doi.org/10.3390/vaccines7040135	journal	September 2019
Collaboration gets the most out of software Morin, Andrew; Eisenbraun, Ben; Key, Jason eLife, Vol. 2 https://doi.org/10.7554/eLife.01456	journal	September 2013

Similar Records

Structural Studies of Neuropilin/Antibody Complexes Provide Insights Into Semaphorin And VEGF Binding

Journal Article · Mon Jun 01 00:00:00 EDT 2009 · EMBO J. 26:4902,2007 · OSTI ID:954008

Mutagenesis of Human Cytomegalovirus Glycoprotein L Disproportionately Disrupts gH/gL/gO over gH/gL/pUL128-131

Journal Article · Tue Aug 10 00:00:00 EDT 2021 · Journal of Virology · OSTI ID:1922084

Inhibition of EBV-mediated membrane fusion by anti-gHgL antibodies

Journal Article · Fri Sep 22 00:00:00 EDT 2017 · Proceedings of the National Academy of Sciences of the United States of America · OSTI ID:1410692

Related Subjects

59 BASIC BIOLOGICAL SCIENCES

Structural basis for HCMV Pentamer recognition by neuropilin 2 and neutralizing antibodies

Citation Formats

References (84)

Similar Records

Related Subjects