Process Intensification for the Biological Production of the Fuel Precursor Butyric Acid from Biomass

Salvachua, Davinia; Saboe, Patrick O.; Nelson, Robert S.; Singer, Christine; McNamara, Ian; del Cerro, Carlos; Chou, Yat-Chen; Mohagheghi, Ali; Peterson, Darren J.; Haugen, Stefan; Cleveland, Nicholas S.; Monroe, Hanna R.; Guarnieri, Michael T.; Tan, Eric C. D.; Beckham, Gregg T.; Karp, Eric M.; Linger, Jeffrey G.

doi:10.1016/j.xcrp.2021.100587

Process Intensification for the Biological Production of the Fuel Precursor Butyric Acid from Biomass

Journal Article · Mon Oct 11 00:00:00 EDT 2021 · Cell Reports Physical Science

DOI:https://doi.org/10.1016/j.xcrp.2021.100587· OSTI ID:1855374

^[1]; ^[1]; Nelson, Robert S. ^[1]; ^[1]; McNamara, Ian ^[1]; del Cerro, Carlos ^[1]; Chou, Yat-Chen ^[1]; Mohagheghi, Ali ^[1]; Peterson, Darren J. ^[1]; Haugen, Stefan ^[1]; ^[1]; Monroe, Hanna R. ^[1]; ^[1]; ^[1]; ^[1]; ^[1]; Linger, Jeffrey G. ^[1]

National Renewable Energy Lab. (NREL), Golden, CO (United States)

The production of fuels from lignocellulosic biomass is key to reduce our reliance on petroleum and to promote a sustainable bioeconomy. Butyric acid (BA) is a promising chemical precursor for the production of renewable diesel and jet fuels. BA can be biologically produced from lignocellulosic sugars. However, challenges associated with product selectivity and recovery must be overcome to achieve industrially relevant metrics. Here, we evaluate various fermentation configurations and demonstrate near-homo-butyrate production by using the biocatalyst Clostridium tyrobutyricum. We also develop an advanced in situ product recovery process based on hybrid extraction-distillation (HED-ISPR) and conduct techno-economic analyses and life cycle assessments. We demonstrate that the HED-ISPR process lowers the overall capital and operating expenses and environmental impact compared to other traditional fermentation processes. Overall, BA minimum product selling price from biomass is 55% of the current BA selling price from petroleum, a significant decrease toward viable renewable fuel production.

View Accepted Manuscript (DOE)

Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1855374

Report Number(s):: NREL/JA-2800-80834; MainId:78612; UUID:30421d37-8d17-4988-a344-2c2bb0aa21d8; MainAdminID:61754

Journal Information:: Cell Reports Physical Science, Journal Name: Cell Reports Physical Science Journal Issue: 10 Vol. 2; ISSN 2666-3864

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

References (55)

Comparative techno‐economic analysis and process design for indirect liquefaction pathways to distillate‐range fuels via biomass‐derived oxygenated intermediates upgrading Tan, Eric C. D.; Snowden‐Swan, Lesley J.; Talmadge, Michael Biofuels, Bioproducts and Biorefining, Vol. 11, Issue 1 https://doi.org/10.1002/bbb.1710	journal	September 2016
An assessment of the potential products and economic and environmental impacts resulting from a billion ton bioeconomy Rogers, Jonathan N.; Stokes, Bryce; Dunn, Jennifer Biofuels, Bioproducts and Biorefining, Vol. 11, Issue 1 https://doi.org/10.1002/bbb.1728	journal	November 2016
Lignocellulose conversion: an introduction to chemistry, process and economics Lange, Jean-Paul Biofuels, Bioproducts and Biorefining, Vol. 1, Issue 1, p. 39-48 https://doi.org/10.1002/bbb.7	journal	January 2007
Extractive fermentation for butyric acid production from glucose byClostridium tyrobutyricum Wu, Zetang; Yang, Shang-Tian Biotechnology and Bioengineering, Vol. 82, Issue 1 https://doi.org/10.1002/bit.10542	journal	February 2003
Butyrate production in continuous culture of Clostridium tyrobutyricum: effect of end-product inhibition Michel-Savin, D.; Marchal, R.; Vandecasteele, J. P. Applied Microbiology and Biotechnology, Vol. 33, Issue 2 https://doi.org/10.1007/BF00176512	journal	May 1990
Arabinose is metabolized via a phosphoketolase pathway in Clostridium acetobutylicum ATCC 824 Servinsky, M. D.; Germane, K. L.; Liu, S. Journal of Industrial Microbiology & Biotechnology, Vol. 39, Issue 12 https://doi.org/10.1007/s10295-012-1186-x	journal	August 2012
Xylose fermentation as a challenge for commercialization of lignocellulosic fuels and chemicals Sànchez Nogué, Violeta; Karhumaa, Kaisa Biotechnology Letters, Vol. 37, Issue 4 https://doi.org/10.1007/s10529-014-1756-2	journal	December 2014
Carboxylate Platform: The MixAlco Process Part 1: Comparison of Three Biomass Conversion Platforms Holtzapple, Mark T.; Granda, Cesar B. Applied Biochemistry and Biotechnology, Vol. 156, Issue 1-3 https://doi.org/10.1007/s12010-008-8466-y	journal	January 2009
Extractive acidogenic fermentation by a supported liquid membrane Nuchnoi, Pakorn; Izawa, Ichiro; Nishio, Naomichi Journal of Fermentation Technology, Vol. 65, Issue 6 https://doi.org/10.1016/0385-6380(87)90013-6	journal	January 1987
Butyric acid production by Clostridium butyricum with integrated extraction and pertraction Zigová, Jana; Šturdı́k, Ernest; Vandák, Dušan Process Biochemistry, Vol. 34, Issue 8 https://doi.org/10.1016/S0032-9592(99)00007-2	journal	October 1999
Supply chain management of butyric acid-derived butanol: Stochastic approach Kwon, Oseok; Han, Jeehoon Applied Energy, Vol. 297 https://doi.org/10.1016/j.apenergy.2021.117119	journal	September 2021
Estimating the higher heating value of biomass fuels from basic analysis data Sheng, Changdong; Azevedo, J. L. T. Biomass and Bioenergy, Vol. 28, Issue 5 https://doi.org/10.1016/j.biombioe.2004.11.008	journal	May 2005
Metabolic flux network analysis of fermentative hydrogen production: Using Clostridium tyrobutyricum as an example Cheng, Hai-Hsuan; Whang, Liang-Ming; Lin, Che-An Bioresource Technology, Vol. 141 https://doi.org/10.1016/j.biortech.2013.03.141	journal	August 2013
Recent advances and strategies in process and strain engineering for the production of butyric acid by microbial fermentation Luo, Hongzhen; Yang, Rongling; Zhao, Yuping Bioresource Technology, Vol. 253 https://doi.org/10.1016/j.biortech.2018.01.007	journal	April 2018
Butyric acid production from spent coffee grounds by engineered Clostridium tyrobutyricum overexpressing galactose catabolism genes He, Feifei; Qin, Shiwen; Yang, Zhi Bioresource Technology, Vol. 304 https://doi.org/10.1016/j.biortech.2020.122977	journal	May 2020
Butyric acid: Applications and recent advances in its bioproduction Jiang, Ling; Fu, Hongxin; Yang, Hopen K. Biotechnology Advances, Vol. 36, Issue 8 https://doi.org/10.1016/j.biotechadv.2018.09.005	journal	December 2018
Hollow fiber membrane life in membrane bioreactors (MBR) Cote, Pierre; Alam, Zamir; Penny, Jeff Desalination, Vol. 288 https://doi.org/10.1016/j.desal.2011.12.026	journal	March 2012
Butyric acid and hydrogen production by Clostridium tyrobutyricum ATCC 25755 and mutants Liu, Xiaoguang; Zhu, Ying; Yang, Shang-Tian Enzyme and Microbial Technology, Vol. 38, Issue 3-4 https://doi.org/10.1016/j.enzmictec.2005.07.008	journal	February 2006
Comparison of metabolic pathway for hydrogen production in wild-type and mutant Clostridium tyrobutyricum strain based on metabolic flux analysis Jiang, Ling; Song, Ping; Zhu, Liying International Journal of Hydrogen Energy, Vol. 38, Issue 5 https://doi.org/10.1016/j.ijhydene.2012.11.050	journal	February 2013
Effect of pH on metabolic pathway shift in fermentation of xylose by Clostridium tyrobutyricum Zhu, Ying; Yang, Shang-Tian Journal of Biotechnology, Vol. 110, Issue 2 https://doi.org/10.1016/j.jbiotec.2004.02.006	journal	May 2004
Carbon material distribution and flux analysis under varying glucose concentrations in hydrogen-producing Clostridium tyrobutyricum JM1 Jo, Ji Hye; Kim, Woong Journal of Biotechnology, Vol. 228 https://doi.org/10.1016/j.jbiotec.2016.04.051	journal	June 2016
Dynamic shear-enhanced membrane filtration: A review of rotating disks, rotating membranes and vibrating systems Jaffrin, Michel Y. Journal of Membrane Science, Vol. 324, Issue 1-2 https://doi.org/10.1016/j.memsci.2008.06.050	journal	October 2008
In situ product recovery: Submerged membranes vs. external loop membranes Carstensen, Frederike; Apel, Andreas; Wessling, Matthias Journal of Membrane Science, Vol. 394-395 https://doi.org/10.1016/j.memsci.2011.11.029	journal	March 2012
The impact of mechanical shear on membrane flux and energy demand Zsirai, T.; Qiblawey, H.; A-Marri, M. J. Journal of Membrane Science, Vol. 516 https://doi.org/10.1016/j.memsci.2016.06.010	journal	October 2016
Metabolic engineering of Clostridium tyrobutyricum for n-butanol production Yu, Mingrui; Zhang, Yali; Tang, I-Ching Metabolic Engineering, Vol. 13, Issue 4 https://doi.org/10.1016/j.ymben.2011.04.002	journal	July 2011
Metabolic engineering of Clostridium tyrobutyricum for enhanced butyric acid production from glucose and xylose Fu, Hongxin; Yu, Le; Lin, Meng Metabolic Engineering, Vol. 40 https://doi.org/10.1016/j.ymben.2016.12.014	journal	March 2017
Novel Strategies for the Production of Fuels, Lubricants, and Chemicals from Biomass Shylesh, Sankaranarayanapillai; Gokhale, Amit A.; Ho, Christopher R. Accounts of Chemical Research, Vol. 50, Issue 10 https://doi.org/10.1021/acs.accounts.7b00354	journal	September 2017
Chain Elongation with Reactor Microbiomes: Open-Culture Biotechnology To Produce Biochemicals Angenent, Largus T.; Richter, Hanno; Buckel, Wolfgang Environmental Science & Technology, Vol. 50, Issue 6 https://doi.org/10.1021/acs.est.5b04847	journal	February 2016
Production of Caproic Acid from Mixed Organic Waste: An Environmental Life Cycle Perspective Chen, Wei-Shan; Strik, David P. B. T. B.; Buisman, Cees J. N. Environmental Science & Technology, Vol. 51, Issue 12 https://doi.org/10.1021/acs.est.6b06220	journal	May 2017
The Techno-Economic Basis for Coproduct Manufacturing To Enable Hydrocarbon Fuel Production from Lignocellulosic Biomass Biddy, Mary J.; Davis, Ryan; Humbird, David ACS Sustainable Chemistry & Engineering, Vol. 4, Issue 6 https://doi.org/10.1021/acssuschemeng.6b00243	journal	May 2016
Construction and Characterization of ack Deleted Mutant of Clostridium tyrobutyricum for Enhanced Butyric Acid and Hydrogen Production Liu, Xiaoguang; Zhu, Ying; Yang, Shang-Tian Biotechnology Progress, Vol. 22, Issue 5 https://doi.org/10.1021/bp060082g	journal	January 2006
Transformation of Biomass into Commodity Chemicals Using Enzymes or Cells Straathof, Adrie J. J. Chemical Reviews, Vol. 114, Issue 3 https://doi.org/10.1021/cr400309c	journal	August 2013
A comprehensive metabolic map for production of bio-based chemicals Lee, Sang Yup; Kim, Hyun Uk; Chae, Tong Un Nature Catalysis, Vol. 2, Issue 1 https://doi.org/10.1038/s41929-018-0212-4	journal	January 2019
Catalytic routes for the conversion of biomass into liquid hydrocarbon transportation fuels Serrano-Ruiz, Juan Carlos; Dumesic, James A. Energy Environ. Sci., Vol. 4, Issue 1 https://doi.org/10.1039/C0EE00436G	journal	January 2011
Bioprivileged molecules: creating value from biomass Shanks, Brent H.; Keeling, Peter L. Green Chemistry, Vol. 19, Issue 14 https://doi.org/10.1039/C7GC00296C	journal	January 2017
In situ recovery of bio-based carboxylic acids Saboe, Patrick O.; Manker, Lorenz P.; Michener, William E. Green Chemistry, Vol. 20, Issue 8 https://doi.org/10.1039/C7GC03747C	journal	January 2018
Catalytic valorization of the acetate fraction of biomass to aromatics and its integration into the carboxylate platform Rozmysłowicz, Bartosz; Yeap, Jher Hau; Elkhaiary, Ahmed M. I. Green Chemistry, Vol. 21, Issue 10 https://doi.org/10.1039/C9GC00513G	journal	January 2019
Tailoring diesel bioblendstock from integrated catalytic upgrading of carboxylic acids: a “fuel property first” approach Huo, Xiangchen; Huq, Nabila A.; Stunkel, Jim Green Chemistry, Vol. 21, Issue 21 https://doi.org/10.1039/C9GC01820D	journal	January 2019
In situ product recovery of bio-based ethyl esters via hybrid extraction-distillation Saboe, Patrick O.; Monroe, Hanna R.; Michener, William E. Green Chemistry, Vol. 21, Issue 19 https://doi.org/10.1039/C9GC01844A	journal	January 2019
Production of jet-fuel-range molecules from biomass-derived mixed acids Jamalzade, Elnaz; Kashkooli, Koorosh; Griffin, Liam Reaction Chemistry & Engineering, Vol. 6, Issue 5 https://doi.org/10.1039/D0RE00401D	journal	January 2021
Performance-advantaged ether diesel bioblendstock production by a priori design Huq, Nabila A.; Huo, Xiangchen; Hafenstine, Glenn R. Proceedings of the National Academy of Sciences, Vol. 116, Issue 52 https://doi.org/10.1073/pnas.1911107116	journal	December 2019
Toward net-zero sustainable aviation fuel with wet waste–derived volatile fatty acids Huq, Nabila A.; Hafenstine, Glenn R.; Huo, Xiangchen Proceedings of the National Academy of Sciences, Vol. 118, Issue 13 https://doi.org/10.1073/pnas.2023008118	journal	March 2021
Biosynthesis of butyric acid by Clostridium tyrobutyricum Huang, Jin; Tang, Wan; Zhu, Shengquan Preparative Biochemistry & Biotechnology, Vol. 48, Issue 5 https://doi.org/10.1080/10826068.2018.1452257	journal	April 2018
The Future of Butyric Acid in Industry Dwidar, Mohammed; Park, Jae-Yeon; Mitchell, Robert J. The Scientific World Journal, Vol. 2012 https://doi.org/10.1100/2012/471417	journal	January 2012
Stabilization Wedges: Solving the Climate Problem for the Next 50 Years with Current Technologies Pacala, S. Science, Vol. 305, Issue 5686 https://doi.org/10.1126/science.1100103	journal	August 2004
Phosphoketolase Pathway for Xylose Catabolism in Clostridium acetobutylicum Revealed by 13C Metabolic Flux Analysis Liu, L.; Zhang, L.; Tang, W. Journal of Bacteriology, Vol. 194, Issue 19 https://doi.org/10.1128/JB.00713-12	journal	August 2012
Deciphering Clostridium tyrobutyricum Metabolism Based on the Whole-Genome Sequence and Proteome Analyses Lee, Joungmin; Jang, Yu-Sin; Han, Mee-Jung mBio, Vol. 7, Issue 3 https://doi.org/10.1128/mBio.00743-16	journal	June 2016
Assessment of metabolic flux distribution in the thermophilic hydrogen producer Caloramator celer as affected by external pH and hydrogen partial pressure Ciranna, Alessandro; Pawar, Sudhanshu S.; Santala, Ville Microbial Cell Factories, Vol. 13, Issue 1 https://doi.org/10.1186/1475-2859-13-48	journal	January 2014
Improved xylose and arabinose utilization by an industrial recombinant Saccharomyces cerevisiae strain using evolutionary engineering Garcia Sanchez, Rosa; Karhumaa, Kaisa; Fonseca, César Biotechnology for Biofuels, Vol. 3, Issue 1 https://doi.org/10.1186/1754-6834-3-13	journal	January 2010
Production of butyric acid from acid hydrolysate of corn husk in fermentation by Clostridium tyrobutyricum: kinetics and process economic analysis Xiao, Zhiping; Cheng, Chu; Bao, Teng Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1165-1	journal	June 2018
Fermentative hydrogen production from agroindustrial lignocellulosic substrates Reginatto, Valeria; Antônio, Regina Vasconcellos Brazilian Journal of Microbiology, Vol. 46, Issue 2 https://doi.org/10.1590/S1517-838246220140111	journal	January 2015
Batch and fed-batch production of butyric acid by Clostridium butyricum ZJUCB He, Guo-qing; Kong, Qing; Chen, Qi-he Journal of Zhejiang University SCIENCE, Vol. 6B, Issue 11 https://doi.org/10.1631/jzus.2005.B1076	journal	January 2005
Understanding biocatalyst inhibition by carboxylic acids Jarboe, Laura R.; Royce, Liam A.; Liu, Ping Frontiers in Microbiology, Vol. 4 https://doi.org/10.3389/fmicb.2013.00272	journal	January 2013
Continuous Fermentation of Clostridium tyrobutyricum with Partial Cell Recycle as a Long-Term Strategy for Butyric Acid Production Du, Jianjun; McGraw, Amy; Lorenz, Nicole Energies, Vol. 5, Issue 8 https://doi.org/10.3390/en5082835	journal	August 2012
Mixed Carboxylic Acid Production by Megasphaera elsdenii from Glucose and Lignocellulosic Hydrolysate Nelson, Robert; Peterson, Darren; Karp, Eric Fermentation, Vol. 3, Issue 1 https://doi.org/10.3390/fermentation3010010	journal	March 2017

Similar Records

Development of Clostridium tyrobutyricum as a Microbial Cell Factory for the Production of Fuel and Chemical Intermediates From Lignocellulosic Feedstocks

Journal Article · Fri Aug 07 00:00:00 EDT 2020 · Frontiers in Energy Research · OSTI ID:1646716

Techno-Economic Evaluation of Cellulosic Ethanol Production Based on Pilot Biorefinery Data: a Case Study of Sweet Sorghum Bagasse Processed via L+SScF

Journal Article · Fri Apr 13 00:00:00 EDT 2018 · BioEnergy Research · OSTI ID:1661471

Related Subjects

09 BIOMASS FUELS
Clostridium butyricum
Clostridium tyrobutyricum
biofuels
bioseparations
carboxylic acids
fermentation
hybrid extraction-distillation
in situ product recovery
life cycle assessment
volatile fatty acids

Process Intensification for the Biological Production of the Fuel Precursor Butyric Acid from Biomass

Citation Formats

References (55)

Similar Records

Related Subjects