Directed Evolution of CRISPR/Cas Systems for Precise Gene Editing

Liu, Rongming; Liang, Liya; Freed, Emily F.; Gill, Ryan T.

doi:10.1016/j.tibtech.2020.07.005

Directed Evolution of CRISPR/Cas Systems for Precise Gene Editing

Journal Article · Wed Aug 19 00:00:00 EDT 2020 · Trends in Biotechnology

DOI:https://doi.org/10.1016/j.tibtech.2020.07.005· OSTI ID:1852615

Liu, Rongming ^[1]; Liang, Liya ^[2]; ^[2]; Gill, Ryan T. ^[3]

Univ. of Colorado, Boulder, CO (United States); Univ. of Colorado, Boulder, CO (United States)
Univ. of Colorado, Boulder, CO (United States)
Univ. of Colorado, Boulder, CO (United States); Technical Univ. of Denmark, Lyngby (Denmark)

CRISPR technology is a universal tool for genome engineering that has revolutionized biotechnology. Recently identified unique CRISPR/Cas systems, as well as re-engineered Cas proteins, have rapidly expanded the functions and applications of CRISPR/Cas systems. The structures of Cas proteins are complex, containing multiple functional domains. These protein domains are evolutionarily conserved polypeptide units that generally show independent structural or functional properties. Here, we propose using protein domains as a new way to classify protein engineering strategies for these proteins and discuss common ways to engineer key domains to modify the functions of CRISPR/Cas systems.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Colorado, Boulder, CO (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC)

Grant/Contract Number:: SC0018368

OSTI ID:: 1852615

Alternate ID(s):: OSTI ID: 1765405

Journal Information:: Trends in Biotechnology, Journal Name: Trends in Biotechnology Journal Issue: 3 Vol. 39; ISSN 0167-7799

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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