Membrane Profiling by Free Flow Electrophoresis and SWATH-MS to Characterize Subcellular Compartment Proteomes in Mesembryanthemum crystallinum

Guo, Qi; Liu, Lei; Yim, Won C.; Cushman, John C.; Barkla, Bronwyn J.

doi:10.3390/ijms22095020

Title: Membrane Profiling by Free Flow Electrophoresis and SWATH-MS to Characterize Subcellular Compartment Proteomes in Mesembryanthemum crystallinum

Journal Article · Sun May 09 00:00:00 EDT 2021 · International Journal of Molecular Sciences (Online)

DOI:https://doi.org/10.3390/ijms22095020· OSTI ID:1851160

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^[1]; Yim, Won C. ^[2];

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Southern Cross Univ., Lismore, NSW (Australia). Faculty of Science and Engineering. Southern Cross Plant Science
Univ. of Nevada, Reno, NV (United States). Dept. of Biochemistry and Molecular Biology

The study of subcellular membrane structure and function facilitates investigations into how biological processes are divided within the cell. However, work in this area has been hampered by the limited techniques available to fractionate the different membranes. Free Flow Electrophoresis (FFE) allows for the fractionation of membranes based on their different surface charges, a property made up primarily of their varied lipid and protein compositions. In this study, high-resolution plant membrane fractionation by FFE, combined with mass spectrometry-based proteomics, allowed the simultaneous profiling of multiple cellular membranes from the leaf tissue of the plant Mesembryanthemum crystallinum. Comparisons of the fractionated membranes’ protein profile to that of known markers for specific cellular compartments sheds light on the functions of proteins, as well as provides new evidence for multiple subcellular localization of several proteins, including those involved in lipid metabolism.

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Research Organization:: Univ. of Nevada, Reno, NV (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0008834

OSTI ID:: 1851160

Journal Information:: International Journal of Molecular Sciences (Online), Vol. 22, Issue 9; ISSN 1422-0067

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
biochemistry & molecular biology
chemistry
subcellular proteomics
membrane fractionation
membrane proteome
marker proteins
subcellular localization
lipid metabolism
lipid biosynthesis
ATPase
mass spectrometry
peptide library

Title: Membrane Profiling by Free Flow Electrophoresis and SWATH-MS to Characterize Subcellular Compartment Proteomes in Mesembryanthemum crystallinum

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