On the Red Antenna States of Photosystem I Mutants from Cyanobacteria Synechocystis PCC 6803

Khmelnitskiy, Anton; Toporik, Hila; Mazor, Yuval; Jankowiak, Ryszard

doi:10.1021/acs.jpcb.0c05201

On the Red Antenna States of Photosystem I Mutants from Cyanobacteria Synechocystis PCC 6803

Journal Article · Thu Aug 27 00:00:00 EDT 2020 · Journal of Physical Chemistry. B, Condensed Matter, Materials, Surfaces, Interfaces and Biophysical Chemistry

DOI:https://doi.org/10.1021/acs.jpcb.0c05201· OSTI ID:1850952

Khmelnitskiy, Anton ^[1]; Toporik, Hila ^[2]; Mazor, Yuval ^[2]; ^[3]

Department of Chemistry, Kansas State University, Manhattan, Kansas 66506, United States; Kansas State University
School of Molecular Sciences and The Biodesign Institute, Arizona State University, Tempe, Arizona 85287, United States
Department of Chemistry, Kansas State University, Manhattan, Kansas 66506, United States; Department of Physics, Kansas State University, Manhattan, Kansas 66506, United States

To identify the molecular composition of the low-energy states in cyanobacterial Photosystem I (PSI) of Synechocystis PCC6803, we focus on high resolution (low-temperature) absorption, emission, resonant, and nonresonant hole burned spectra obtained for wild-type (WT) PSI and three PSI mutants. In the Red_a mutant, the B33 chlorophyll (Chl) is added to the B31-B32 dimer; in Red_b, histidine 95 (His95) on PsaB (which coordinates Mg in the B7 Chl within the His95-B7-A31-A32- cluster) is replaced with glutamine (Gln), while in the Red_ab mutant, both mutations are made. We show that the C706 state (B31-B32) changes to the C710 state (B31-B32-B33) in both Red_a and Red_ab mutants, while the C707 state in WT Synechocystis (localized on the His95-B7-A31-A32 cluster) is modified to C716 in both Red_b and Red_ab. Excitation energy transfer from C706 to the C714 trap in the WT PSI and Red_b mutant is hampered as reflected by a weak emission at 712 nm. Large electron-phonon coupling strength (exposed via resonant hole-burned spectra) is consistent with a strong mixing of excited states with intermolecular charge transfer states leading to significantly red-shifted emission spectra. We conclude that excitation energy transfer in PSI is controlled by fine-tuning the electronic states of a small number of highly conserved red states. Finally, we show that mutations modify the protein potential energy landscape as revealed by different shapes and shifts of the blue- and red-shifted antiholes.

View Accepted Manuscript (DOE)

Research Organization:: Kansas State University, Manhattan, KS (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division

Grant/Contract Number:: SC0006678

OSTI ID:: 1850952

Alternate ID(s):: OSTI ID: 1865091

Journal Information:: Journal of Physical Chemistry. B, Condensed Matter, Materials, Surfaces, Interfaces and Biophysical Chemistry, Journal Name: Journal of Physical Chemistry. B, Condensed Matter, Materials, Surfaces, Interfaces and Biophysical Chemistry Journal Issue: 39 Vol. 124; ISSN 1520-6106

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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On the Red Antenna States of Photosystem I Mutants from Cyanobacteria Synechocystis PCC 6803

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