Phosphoglucoisomerase Is an Important Regulatory Enzyme in Partitioning Carbon out of the Calvin-Benson Cycle

Preiser, Alyssa L.; Banerjee, Aparajita; Weise, Sean E.; Renna, Luciana; Brandizzi, Federica; Sharkey, Thomas D.

doi:10.3389/fpls.2020.580726

Phosphoglucoisomerase Is an Important Regulatory Enzyme in Partitioning Carbon out of the Calvin-Benson Cycle

Journal Article · Wed Dec 09 23:00:00 EST 2020 · Frontiers in Plant Science

DOI:https://doi.org/10.3389/fpls.2020.580726· OSTI ID:1849999

Preiser, Alyssa L. ^[1]; Banerjee, Aparajita ^[2]; Weise, Sean E. ^[3]; Renna, Luciana ^[3]; Brandizzi, Federica ^[4]; Sharkey, Thomas D. ^[5]

Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory; Michigan State Univ., East Lansing, MI (United States). Dept. of Biochemistry and Molecular Biology; Michigan State Univ., East Lansing, MI (United States)
Univ., East Lansing, MI (United States). Dept. of Biochemistry and Molecular Biology
Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory
Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory; Michigan State Univ., East Lansing, MI (United States). Dept. of Plant Biology
Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory; Michigan State Univ., East Lansing, MI (United States). Dept. of Biochemistry and Molecular Biology; Michigan State Univ., East Lansing, MI (United States). Plant Resilience Inst.

Phosphoglucoisomerase (PGI) isomerizes fructose 6-phosphate (F6P) and glucose 6-phosphate (G6P) in starch and sucrose biosynthesis. Both plastidic and cytosolic isoforms are found in plant leaves. Using recombinant enzymes and isolated chloroplasts, we have characterized the plastidic and cytosolic isoforms of PGI. We have found that the Arabidopsis plastidic PGI K_m for G6P is three-fold greater compared to that for F6P and that erythrose 4-phosphate is a key regulator of PGI activity. Additionally, the K_m of spinach plastidic PGI can be dynamically regulated in the dark compared to the light and increases by 200% in the dark. We also found that targeting Arabidopsis cytosolic PGI into plastids of Nicotiana tabacum disrupts starch accumulation and degradation. Our results, in combination with the observation that plastidic PGI is not in equilibrium, indicates that PGI is an important regulatory enzyme that restricts flow and acts as a one-way valve preventing backflow of G6P into the Calvin-Benson cycle. We propose the PGI may be manipulated to improve flow of carbon to desired targets of biotechnology.

View Accepted Manuscript (DOE)

Research Organization:: Michigan State Univ., East Lansing, MI (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: FG02-91ER20021

OSTI ID:: 1849999

Journal Information:: Frontiers in Plant Science, Journal Name: Frontiers in Plant Science Vol. 11; ISSN 1664-462X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Phosphoglucoisomerase Is an Important Regulatory Enzyme in Partitioning Carbon out of the Calvin-Benson Cycle

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References (47)

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