Drug Resistance in Filarial Parasites Does Not Affect Mosquito Vectorial Capacity

Neff, Erik; Evans, Christopher C.; Jimenez Castro, Pablo D.; Kaplan, Ray M.; Dharmarajan, Guha

doi:10.3390/pathogens10010002

Drug Resistance in Filarial Parasites Does Not Affect Mosquito Vectorial Capacity

Journal Article · Tue Dec 22 00:00:00 EST 2020 · Pathogens

DOI:https://doi.org/10.3390/pathogens10010002· OSTI ID:1848978

^[1]; Evans, Christopher C. ^[2]; ^[3]; Kaplan, Ray M. ^[2]; ^[4]

Savannah River Site (SRS), Aiken, SC (United States). Savannah River Ecology Lab. (SREL); Savannah River Site (SRS), Aiken, SC (United States). Savannah River Ecology Lab. (SREL)
Univ. of Georgia, Athens, GA (United States). College of Veterinary Medicine. Dept. of Infectious Diseases
Univ. of Georgia, Athens, GA (United States). College of Veterinary Medicine. Dept. of Infectious Diseases; Universidad Nacional de Colombia, Bogotá (Colombia). Grupo de Parasitología Veterinaria
Savannah River Site (SRS), Aiken, SC (United States). Savannah River Ecology Lab. (SREL)

Parasite drug resistance presents a major obstacle to controlling and eliminating vector-borne diseases affecting humans and animals. While vector-borne disease dynamics are affected by factors related to parasite, vertebrate host and vector, research on drug resistance in filarial parasites has primarily focused on the parasite and vertebrate host, rather than the mosquito. However, we expect that the physiological costs associated with drug resistance would reduce the fitness of drug-resistant vs. drug-susceptible parasites in the mosquito wherein parasites are not exposed to drugs. Here we test this hypothesis using four isolates of the dog heartworm (Dirofilaria immitis)—two drug susceptible and two drug resistant—and two vectors—the yellow fever mosquito (Aedes aegypti) and the Asian tiger mosquito (Ae. albopictus)—as our model system. Our data indicated that while vector species had a significant effect on vectorial capacity, there was no significant difference in the vectorial capacity of mosquitoes infected with drug-resistant vs. drug-susceptible parasites. Consequently, contrary to expectations, our data indicate that drug resistance in D. immitis does not appear to reduce the transmission efficiency of these parasites, and thus the spread of drug-resistant parasites in the vertebrate population is unlikely to be mitigated by reduced fitness in the mosquito vector.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Georgia, Athens, GA (United States)

Sponsoring Organization:: USDOE Office of Environmental Management (EM)

Grant/Contract Number:: EM0004391

OSTI ID:: 1848978

Journal Information:: Pathogens, Journal Name: Pathogens Journal Issue: 1 Vol. 10; ISSN 2076-0817; ISSN PATHCD

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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mosquito
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Drug Resistance in Filarial Parasites Does Not Affect Mosquito Vectorial Capacity

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