Regulation of dhurrin pathway gene expression during Sorghum&nbsp;bicolor development

Gleadow, Roslyn M.; McKinley, Brian A.; Blomstedt, Cecilia K.; Lamb, Austin C.; Møller, Birger Lindberg; Mullet, John E.

doi:10.1007/s00425-021-03774-2

Regulation of dhurrin pathway gene expression during Sorghum bicolor development

Journal Article · Thu Nov 11 00:00:00 EST 2021 · Planta

DOI:https://doi.org/10.1007/s00425-021-03774-2· OSTI ID:1846171

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Monash Univ., Melbourne, VIC (Australia); Department of Plant Biochemistry and Biophysics, Texas A&M University
Texas A & M Univ., College Station, TX (United States)
Monash Univ., Melbourne, VIC (Australia)
Univ. of Copenhagen (Denmark)

Plant defence models evaluate the costs and benefits of resource investments at different stages in the life cycle. Poor understanding of the molecular regulation of defence deployment and remobilization hampers accuracy of the predictions. Cyanogenic glucosides, such as dhurrin are phytoanticipins that release hydrogen cyanide upon bio-activation. In this study, RNA-seq was used to investigate the expression of genes involved in the biosynthesis, bio-activation and recycling of dhurrin in Sorghum bicolor. Genes involved in dhurrin biosynthesis were highly expressed in all young developing vegetative tissues (leaves, leaf sheath, roots, stems), tiller buds and imbibing seeds and showed gene specific peaks of expression in leaves during diel cycles. Genes involved in dhurrin bio-activation were expressed early in organ development with organ-specific expression patterns. Genes involved in recycling were expressed at similar levels in the different organ during development, although post-floral initiation when nutrients are remobilized for grain filling, expression of GSTL1 decreased > tenfold in leaves and NITB2 increased > tenfold in stems. Results are consistent with the establishment of a pre-emptive defence in young tissues and regulated recycling related to organ senescence and increased demand for nitrogen during grain filling. This detailed characterization of the transcriptional regulation of dhurrin biosynthesis, bioactivation and remobilization genes during organ and plant development will aid elucidation of gene regulatory networks and signalling pathways that modulate gene expression and dhurrin levels. In-depth knowledge of dhurrin metabolism could improve the yield, nitrogen use efficiency and stress resilience of Sorghum.

View Accepted Manuscript (DOE)

Research Organization:: Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)

Sponsoring Organization:: Australian Research Council; Novo Nordisk Foundation; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018409

OSTI ID:: 1846171

Journal Information:: Planta, Journal Name: Planta Journal Issue: 6 Vol. 254; ISSN 0032-0935

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Cyanogenic glucosides
Diel cycle
Hydrogen cyanide
Ontogeny
Plant defence
RNA-seq
Sorghum bicolor

Regulation of dhurrin pathway gene expression during Sorghum bicolor development

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