Electrochemically Driven Photosynthetic Electron Transport in Cyanobacteria Lacking Photosystem II
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- School of Molecular Sciences, Arizona State University, Tempe, Arizona 85287, United States, Biodesign Institute Center for Applied Structural Discovery, Arizona State University, Tempe, Arizona 85287, United States, Biodesign Institute Swette Center for Environmental Biotechnology, Arizona State University, Tempe, Arizona 85287, United States
- Biodesign Institute Center for Applied Structural Discovery, Arizona State University, Tempe, Arizona 85287, United States, Engineering Center for Negative Carbon Emmisions, at Arizona State University, Tempe, Arizona 85281, United States
- School of Molecular Sciences, Arizona State University, Tempe, Arizona 85287, United States, Julie Ann Wrigley Global Institute of Sustainability and Innovation, Arizona State University, Tempe Arizona 85287, United States
- School of Sustainable Engineering and the Built Environment, Arizona State University, Tempe, Arizona 85287, United States, Biodesign Institute Swette Center for Environmental Biotechnology, Arizona State University, Tempe, Arizona 85287, United States
- School of Life Sciences, Arizona State University, Tempe, Arizona 85287, United States
- Biodesign Institute Swette Center for Environmental Biotechnology, Arizona State University, Tempe, Arizona 85287, United States, School for Engineering of Matter, Transport and Energy, Arizona State University, Tempe, Arizona 85287, United States
- School of Molecular Sciences, Arizona State University, Tempe, Arizona 85287, United States, Biodesign Institute Center for Applied Structural Discovery, Arizona State University, Tempe, Arizona 85287, United States
Light-activated photosystem II (PSII) carries out the critical step of splitting water in photosynthesis. However, PSII is susceptible to light-induced damage. Here, results are presented from a novel microbial electro-photosynthetic system (MEPS) that uses redox mediators in conjunction with an electrode to drive electron transport in live Synechocystis (ΔpsbB) cells lacking PSII. MEPSgenerated, light-dependent current increased with light intensity up to 2050 μmol photons m–2 s–1, which yielded a delivery rate of 113 μmol electrons h–1 mg-chl–1 and an average current density of 150 A m–2 s–1 mg-chl–1. P700+ re-reduction kinetics demonstrated that initial rates exceeded wildtype PSII-driven electron delivery. The electron delivery occurs ahead of the cytochrome b6f complex to enable both NADPH and ATP production. This work demonstrates an electrochemical system that can drive photosynthetic electron transport, provides a platform for photosynthetic foundational studies, and has the potential for improving photosynthetic performance at high light intensities.
- Research Organization:
- Arizona State Univ. Biodesign Inst., Tempe, AZ (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- Grant/Contract Number:
- FG02-03ER15393; SC0001016
- OSTI ID:
- 1845163
- Alternate ID(s):
- OSTI ID: 1846243
- Journal Information:
- Journal of the American Chemical Society, Journal Name: Journal of the American Chemical Society Vol. 144 Journal Issue: 7; ISSN 0002-7863
- Publisher:
- American Chemical SocietyCopyright Statement
- Country of Publication:
- United States
- Language:
- English
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