Engineering a Non–Natural Photoenzyme for Improved Photon Efficiency

Nicholls, Bryce T.; Oblinsky, Daniel G.; Kurtoic, Sarah I.; Grosheva, Daria; Ye, Yuxuan; Scholes, Gregory D.; Hyster, Todd K.

doi:10.1002/anie.202113842

Engineering a Non–Natural Photoenzyme for Improved Photon Efficiency

Journal Article · Fri Nov 05 00:00:00 EDT 2021 · Angewandte Chemie (International Edition)

DOI:https://doi.org/10.1002/anie.202113842· OSTI ID:1843580

Nicholls, Bryce T. ^[1]; Oblinsky, Daniel G. ^[2]; Kurtoic, Sarah I. ^[2]; Grosheva, Daria ^[2]; Ye, Yuxuan ^[3]; Scholes, Gregory D. ^[2]; ^[3]

Cornell Univ., Ithaca, NY (United States); Princeton Univ., NJ (United States); Cornell University
Princeton Univ., NJ (United States)
Cornell Univ., Ithaca, NY (United States)

We developed a novel HTS engineering platform to optimize photoenzymatic activity. The improvements in variants were correlated to an increase in enzymatic photon efficiency. Here, transient absorption spectroscopy revealed a shift from a stepwise to a concerted mechanism. The platform was expanded to improve the synthesis of γ, δ, ϵ-lactams, and acyclic amides.

View Accepted Manuscript (DOE)

Research Organization:: Cornell Univ., Ithaca, NY (United States); Princeton Univ., NJ (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0019370

OSTI ID:: 1843580

Alternate ID(s):: OSTI ID: 1833900

Journal Information:: Angewandte Chemie (International Edition), Journal Name: Angewandte Chemie (International Edition) Journal Issue: 2 Vol. 61; ISSN 1433-7851

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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