Rosette core fungal resistance in Arabidopsis thaliana

Dai, Yanwan; Ogilvie, Huw A.; Liu, Yuan; Huang, Michael; Markillie, Lye Meng; Mitchell, Hugh D.; Borrego, Eli J.; Kolomiets, Michael V.; Gaffrey, Matthew J.; Orr, Galya; Chehab, E. Wassim; Mao, Wan-Ting; Braam, Janet

doi:10.1007/s00425-019-03273-5

Rosette core fungal resistance in Arabidopsis thaliana

Journal Article · Mon Sep 16 00:00:00 EDT 2019 · Planta

DOI:https://doi.org/10.1007/s00425-019-03273-5· OSTI ID:1842955

^[1]; Ogilvie, Huw A. ^[1]; Liu, Yuan ^[1]; Huang, Michael ^[1]; Markillie, Lye Meng ^[2]; Mitchell, Hugh D. ^[2]; Borrego, Eli J. ^[3]; Kolomiets, Michael V. ^[3]; Gaffrey, Matthew J. ^[2]; ^[2]; Chehab, E. Wassim ^[1]; Mao, Wan-Ting ^[4]; ^[1]

Rice Univ., Houston, TX (United States)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Texas A & M Univ., College Station, TX (United States)
National Chung Hsing Univ., Taichung (Taiwan)

Main conclusion: Unlike rosette leaves, the mature Arabidopsis rosette core can display full resistance to Botrytis cinerea revealing the importance for spatial and developmental aspects of plant fungal resistance. Abstract: Arabidopsis thaliana is a model host to investigate plant defense against fungi. However, many of the reports investigating Arabidopsis fungal defense against the necrotrophic fungus, Botrytis cinerea, utilize rosette leaves as host tissue. Here we report organ-dependent differences in B. cinerea resistance of Arabidopsis. Although wild-type Arabidopsis rosette leaves mount a jasmonate-dependent defense that slows fungal growth, this defense is incapable of resisting fungal devastation. In contrast, as the fungus spreads through infected leaf petioles towards the plant center, or rosette core, there is a jasmonate- and age-dependent fungal penetration blockage into the rosette core. In this paper, we report evidence for induced and preformed resistance in the rosette core, as direct rosette core inoculation can also result in resistance, but at a lower penetrance relative to infections that approach the core from infected leaf petioles. The Arabidopsis rosette core displays a distinct transcriptome relative to other plant organs, and BLADE ON PETIOLE (BOP) transcripts are abundant in the rosette core. The BOP genes, with known roles in abscission zone formation, are required for full Arabidopsis rosette core B. cinerea resistance, suggesting a possible role for BOP-dependent modifications that may help to restrict fungal susceptibility of the rosette core. Finally, we demonstrate that cabbage and cauliflower, common Brassicaceae crops, also display leaf susceptibility and rosette core resistance to B. cinerea that can involve leaf abscission. Thus, spatial and developmental aspects of plant host resistance play critical roles in resistance to necrotrophic fungal pathogens and are important to our understanding of plant defense mechanisms.

View Accepted Manuscript (DOE)

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)

Sponsoring Organization:: China Scholarship Council (CSC); USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1842955

Alternate ID(s):: OSTI ID: 1605514
OSTI ID: 1842956

Report Number(s):: PNNL-SA--147546

Journal Information:: Planta, Journal Name: Planta Journal Issue: 6 Vol. 250; ISSN 0032-0935

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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