Regulation of Cell Wall Assembly: Myosin and Exocyst Involvement in Cellulose Synthase Delivery to the Plasma Membrane

Technical Report · Tue Apr 19 04:00:00 EDT 2022

DOI:https://doi.org/10.2172/1840725· OSTI ID:1840725

Staiger, Christopher J. ^[1]

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1. Purdue Univ., West Lafayette, IN (United States); Purdue University

Cellulose is the most abundant biopolymer on the planet and is produced in the primary and secondary cell wall of terrestrial plants by a plasma membrane (PM)-localized, multimeric protein complex. The catalytic enzyme, or cellulose synthase, belongs to a multigene family known as CESA. It is generally accepted that the behavior and trajectories of cellulose synthase complexes are oriented by the position of cortical microtubules, but how exactly these complexes are delivered and recycled from the PM remains poorly understood. Limited evidence suggests that microtubules determine the site for delivery of new complexes; however, abolishing microtubules with the inhibitor oryzalin has absolutely no effect on rates of delivery. Thus, there is a pressing need to explore the contribution of another component of the cortical cytoskeleton, actin filaments and the associated motor protein myosin, to the delivery and dynamics of CESA at the PM.

• Research Organization: Purdue Univ., West Lafayette, IN (United States)
• Sponsoring Organization: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division
• DOE Contract Number: FG02-04ER15526
• OSTI ID: 1840725
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
actin cytoskeleton
cell wall assembly
cellulose
cellulose synthase
exocyst complex
myosin