The human coronavirus HCoV-229E S-protein structure and receptor binding

Li, Zhijie; Tomlinson, Aidan C. A.; Wong, Alan H. M.; Zhou, Dongxia; Desforges, Marc; Talbot, Pierre J.; Benlekbir, Samir; Rubinstein, John L.; Rini, James M.

doi:10.7554/elife.51230

The human coronavirus HCoV-229E S-protein structure and receptor binding

Journal Article · Fri Oct 25 00:00:00 EDT 2019 · eLife

DOI:https://doi.org/10.7554/elife.51230· OSTI ID:1837244

^[1]; Tomlinson, Aidan C. A. ^[2]; Wong, Alan H. M. ^[2]; Zhou, Dongxia ^[1]; Desforges, Marc ^[3]; Talbot, Pierre J. ^[3]; Benlekbir, Samir ^[4]; ^[5]; ^[6]

Univ. of Toronto, ON (Canada). Dept. of Molecular Genetics
Univ. of Toronto, ON (Canada). Dept. of Biochemistry
Univ. of Quebec, Quebec City, QC (Canada). Lab. of Neuroimmunovirology. INRS-Inst. Armand-Frappier. Inst. National de la Recherche Scientifique
The Hospital for Sick Children Research Inst., Toronto, ON (Canada). Molecular Medicine Program
Univ. of Toronto, ON (Canada). Dept. of Biochemistry. Dept. of Medical Biophysics; The Hospital for Sick Children Research Inst., Toronto, ON (Canada). Molecular Medicine Program
Univ. of Toronto, ON (Canada). Dept. of Molecular Genetics. Dept. of Biochemistry

The coronavirus S-protein mediates receptor binding and fusion of the viral and host cell membranes. In HCoV-229E, its receptor binding domain (RBD) shows extensive sequence variation but how S-protein function is maintained is not understood. Reported are the X-ray crystal structures of Class III-V RBDs in complex with human aminopeptidase N (hAPN), as well as the electron cryomicroscopy structure of the 229E S-protein. The structures show that common core interactions define the specificity for hAPN and that the peripheral RBD sequence variation is accommodated by loop plasticity. The results provide insight into immune evasion and the cross-species transmission of 229E and related coronaviruses. We also find that the 229E S-protein can expose a portion of its helical core to solvent. This is undoubtedly facilitated by hydrophilic subunit interfaces that we show are conserved among coronaviruses. These interfaces likely play a role in the S-protein conformational changes associated with membrane fusion.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC)

OSTI ID:: 1837244

Alternate ID(s):: OSTI ID: 1845544

Journal Information:: eLife, Journal Name: eLife Vol. 8; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: ENGLISH

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