A CLC-ec1 mutant reveals global conformational change and suggests a unifying mechanism for the CLC

Chavan, Tanmay S.; Cheng, Ricky C.; Jiang, Tao; Mathews, Irimpan I.; Stein, Richard A.; Koehl, Antoine; Mchaourab, Hassane S.; Tajkhorshid, Emad; Maduke, Merritt

doi:10.7554/eLife.53479

Title: A CLC-ec1 mutant reveals global conformational change and suggests a unifying mechanism for the CLC

Journal Article · Wed Apr 01 00:00:00 EDT 2020 · eLIFE J.

DOI:https://doi.org/10.7554/eLife.53479· OSTI ID:1834831

Chavan, Tanmay S.; Cheng, Ricky C.; Jiang, Tao; Mathews, Irimpan I.; Stein, Richard A.; Koehl, Antoine; Mchaourab, Hassane S.; Tajkhorshid, Emad; Maduke, Merritt

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Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: DOE - Office Of Science; AHA; DOE - BASIC ENERGY SCIENCES; National Institutes of Health (NIH); UNIVERSITY

OSTI ID:: 1834831

Journal Information:: eLIFE J., Vol. 9

Country of Publication:: United States

Language:: ENGLISH

References (105)

13C NMR detects conformational change in the 100-kD membrane transporter ClC-ec1 Abraham, Sherwin J.; Cheng, Ricky C.; Chew, Thomas A. Journal of Biomolecular NMR, Vol. 61, Issue 3-4 https://doi.org/10.1007/s10858-015-9898-7	journal	January 2015
Separate Ion Pathways in a Cl ⁻ /H ⁺ Exchanger Accardi, Alessio; Walden, Michael; Nguitragool, Wang The Journal of General Physiology, Vol. 126, Issue 6 https://doi.org/10.1085/jgp.200509417	journal	November 2005
Structure and gating of CLC channels and exchangers: Structure and gating of CLC channels and exchangers Accardi, Alessio The Journal of Physiology, Vol. 593, Issue 18 https://doi.org/10.1113/JP270575	journal	July 2015
Secondary active transport mediated by a prokaryotic homologue of ClC Cl- channels Accardi, Alessio; Miller, Christopher Nature, Vol. 427, Issue 6977 https://doi.org/10.1038/nature02314	journal	February 2004
Conformational changes required for H+/Cl− exchange mediated by a CLC transporter Basilio, Daniel; Noack, Kristin; Picollo, Alessandra Nature Structural & Molecular Biology, Vol. 21, Issue 5 https://doi.org/10.1038/nsmb.2814	journal	April 2014
A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl<sup>-</sup> Channels and Transporters Basilio, Daniel; Accardi, Alessio Journal of Visualized Experiments, Issue 98 https://doi.org/10.3791/52369	journal	January 2015
Analysis of CLCNKB mutations at dimer‐interface, calcium‐binding site, and pore reveals a variety of functional alterations in ClC‐Kb channel leading to Bartter syndrome Bignon, Yohan; Sakhi, Imene; Bitam, Sara Human Mutation, Vol. 41, Issue 4 https://doi.org/10.1002/humu.23962	journal	December 2019
Improved Sensitivity for Long-Distance Measurements in Biomolecules: Five-Pulse Double Electron–Electron Resonance Borbat, Peter P.; Georgieva, Elka R.; Freed, Jack H. The Journal of Physical Chemistry Letters, Vol. 4, Issue 1 https://doi.org/10.1021/jz301788n	journal	December 2012
The global analysis of DEER data Brandon, Suzanne; Beth, Albert H.; Hustedt, Eric J. Journal of Magnetic Resonance, Vol. 218 https://doi.org/10.1016/j.jmr.2012.03.006	journal	May 2012
Crystallizing membrane proteins using lipidic mesophases Caffrey, Martin; Cherezov, Vadim Nature Protocols, Vol. 4, Issue 5 https://doi.org/10.1038/nprot.2009.31	journal	April 2009
CAVER 3.0: A Tool for the Analysis of Transport Pathways in Dynamic Protein Structures Chovancova, Eva; Pavelka, Antonin; Benes, Petr PLoS Computational Biology, Vol. 8, Issue 10 https://doi.org/10.1371/journal.pcbi.1002708	journal	October 2012
Inward-facing conformation of glutamate transporters as revealed by their inverted-topology structural repeats Crisman, T. J.; Qu, S.; Kanner, B. I. Proceedings of the National Academy of Sciences, Vol. 106, Issue 49 https://doi.org/10.1073/pnas.0908570106	journal	November 2009
Particle mesh Ewald: An N ⋅log( N ) method for Ewald sums in large systems Darden, Tom; York, Darrin; Pedersen, Lee The Journal of Chemical Physics, Vol. 98, Issue 12 https://doi.org/10.1063/1.464397	journal	June 1993
Inverted Topologies in Membrane Proteins: a Mini-Review Duran, Amanda M.; Meiler, Jens Computational and Structural Biotechnology Journal, Vol. 8, Issue 11 https://doi.org/10.5936/csbj.201308004	journal	August 2013
Adaptive Partitioning QM/MM for Molecular Dynamics Simulations: 6. Proton Transport through a Biological Channel Duster, Adam W.; Garza, Christina M.; Aydintug, Baris O. Journal of Chemical Theory and Computation, Vol. 15, Issue 2 https://doi.org/10.1021/acs.jctc.8b01128	journal	November 2018
X-ray structure of a ClC chloride channel at 3.0 Å reveals the molecular basis of anion selectivity Dutzler, Raimund; Campbell, Ernest B.; Cadene, Martine Nature, Vol. 415, Issue 6869 https://doi.org/10.1038/415287a	journal	January 2002
Gating the Selectivity Filter in ClC Chloride Channels Dutzler, R. Science, Vol. 300, Issue 5616 https://doi.org/10.1126/science.1082708	journal	March 2003
Substrate-driven conformational changes in ClC-ec1 observed by fluorine NMR Elvington, Shelley M.; Liu, Corey W.; Maduke, Merritt C. The EMBO Journal, Vol. 28, Issue 20 https://doi.org/10.1038/emboj.2009.259	journal	September 2009
Coot model-building tools for molecular graphics Emsley, Paul; Cowtan, Kevin Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132 https://doi.org/10.1107/S0907444904019158	journal	November 2004
Cysteine Accessibility in ClC-0 Supports Conservation of the ClC Intracellular Vestibule Engh, Anita M.; Maduke, Merritt The Journal of General Physiology, Vol. 125, Issue 6 https://doi.org/10.1085/jgp.200509258	journal	May 2005
Conservation of Chloride Channel Structure Revealed by an Inhibitor Binding Site in ClC-1 Estévez, Raúl; Schroeder, Björn C.; Accardi, Alessio Neuron, Vol. 38, Issue 1 https://doi.org/10.1016/S0896-6273(03)00168-5	journal	April 2003
Scaling and assessment of data quality Evans, Philip Acta Crystallographica Section D Biological Crystallography, Vol. 62, Issue 1, p. 72-82 https://doi.org/10.1107/S0907444905036693	journal	December 2005
Electrostatics of Ion Stabilization in a ClC Chloride Channel Homologue from Escherichia coli Faraldo-Gómez, José D.; Roux, Benoı̂t Journal of Molecular Biology, Vol. 339, Issue 4 https://doi.org/10.1016/j.jmb.2004.04.023	journal	June 2004
Structure of a Eukaryotic CLC Transporter Defines an Intermediate State in the Transport Cycle Feng, Liang; Campbell, Ernest B.; Hsiung, Yichun Science, Vol. 330, Issue 6004 https://doi.org/10.1126/science.1195230	journal	September 2010
Molecular mechanism of proton transport in CLC Cl-/H+ exchange transporters Feng, L.; Campbell, E. B.; MacKinnon, R. Proceedings of the National Academy of Sciences, Vol. 109, Issue 29 https://doi.org/10.1073/pnas.1205764109	journal	July 2012
Mechanism for alternating access in neurotransmitter transporters Forrest, L. R.; Zhang, Y. -W.; Jacobs, M. T. Proceedings of the National Academy of Sciences, Vol. 105, Issue 30 https://doi.org/10.1073/pnas.0804659105	journal	July 2008
The structural basis of secondary active transport mechanisms Forrest, Lucy R.; Krämer, Reinhard; Ziegler, Christine Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1807, Issue 2 https://doi.org/10.1016/j.bbabio.2010.10.014	journal	February 2011
Structural Symmetry in Membrane Proteins Forrest, Lucy R. Annual Review of Biophysics, Vol. 44, Issue 1 https://doi.org/10.1146/annurev-biophys-051013-023008	journal	June 2015
The Rocking Bundle: A Mechanism for Ion-Coupled Solute Flux by Symmetrical Transporters Forrest, Lucy R.; Rudnick, Gary Physiology, Vol. 24, Issue 6 https://doi.org/10.1152/physiol.00030.2009	journal	December 2009
Water access points and hydration pathways in CLC H ⁺ /Cl ⁻ transporters Han, Wei; Cheng, Ricky C.; Maduke, Merritt C. Proceedings of the National Academy of Sciences, Vol. 111, Issue 5 https://doi.org/10.1073/pnas.1317890111	journal	December 2013
Dent Disease with Mutations in OCRL1 Hoopes, Richard R.; Shrimpton, Antony E.; Knohl, Stephen J. The American Journal of Human Genetics, Vol. 76, Issue 2 https://doi.org/10.1086/427887	journal	February 2005
Canonical dynamics: Equilibrium phase-space distributions Hoover, William G. Physical Review A, Vol. 31, Issue 3 https://doi.org/10.1103/PhysRevA.31.1695	journal	March 1985
X-exome sequencing of 405 unresolved families identifies seven novel intellectual disability genes Hu, H.; Haas, S. A.; Chelly, J. Molecular Psychiatry, Vol. 21, Issue 1 https://doi.org/10.1038/mp.2014.193	journal	February 2015
CHARMM36 all-atom additive protein force field: Validation based on comparison to NMR data Huang, Jing; MacKerell, Alexander D. Journal of Computational Chemistry, Vol. 34, Issue 25 https://doi.org/10.1002/jcc.23354	journal	July 2013
VMD: Visual molecular dynamics Humphrey, William; Dalke, Andrew; Schulten, Klaus Journal of Molecular Graphics, Vol. 14, Issue 1 https://doi.org/10.1016/0263-7855(96)00018-5	journal	February 1996
Confidence Analysis of DEER Data and Its Structural Interpretation with Ensemble-Biased Metadynamics Hustedt, Eric J.; Marinelli, Fabrizio; Stein, Richard A. Biophysical Journal, Vol. 115, Issue 7 https://doi.org/10.1016/j.bpj.2018.08.008	journal	October 2018
Arginine-Agmatine Antiporter in Extreme Acid Resistance in Escherichia coli Iyer, Ram; Williams, Carole; Miller, Christopher Journal of Bacteriology, Vol. 185, Issue 22 https://doi.org/10.1128/JB.185.22.6556-6561.2003	journal	November 2003
Simple Allosteric Model for Membrane Pumps Jardetzky, Oleg Nature, Vol. 211, Issue 5052 https://doi.org/10.1038/211969a0	journal	August 1966
CLC Chloride Channels and Transporters: Structure, Function, Physiology, and Disease Jentsch, Thomas J.; Pusch, Michael Physiological Reviews, Vol. 98, Issue 3 https://doi.org/10.1152/physrev.00047.2017	journal	July 2018
DEER Distance Measurements on Proteins Jeschke, Gunnar Annual Review of Physical Chemistry, Vol. 63, Issue 1 https://doi.org/10.1146/annurev-physchem-032511-143716	journal	May 2012
Distance measurements on spin-labelled biomacromolecules by pulsed electron paramagnetic resonance Jeschke, Gunnar; Polyhach, Yevhen Physical Chemistry Chemical Physics, Vol. 9, Issue 16 https://doi.org/10.1039/b614920k	journal	January 2007
Molecular Basis for Differential Anion Binding and Proton Coupling in the Cl ^– /H ⁺ Exchanger ClC-ec1 Jiang, Tao; Han, Wei; Maduke, Merritt Journal of the American Chemical Society, Vol. 138, Issue 9 https://doi.org/10.1021/jacs.5b12062	journal	February 2016
Comparison of simple potential functions for simulating liquid water Jorgensen, William L.; Chandrasekhar, Jayaraman; Madura, Jeffry D. The Journal of Chemical Physics, Vol. 79, Issue 2 https://doi.org/10.1063/1.445869	journal	July 1983
XDS Kabsch, Wolfgang Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2 https://doi.org/10.1107/S0907444909047337	journal	January 2010
Loss of the chloride channel ClC-7 leads to lysosomal storage disease and neurodegeneration Kasper, Dagmar; Planells-Cases, Rosa; Fuhrmann, Jens C. The EMBO Journal, Vol. 24, Issue 5 https://doi.org/10.1038/sj.emboj.7600576	journal	February 2005
Revealing an outward-facing open conformational state in a CLC Cl–/H+ exchange transporter Khantwal, Chandra M.; Abraham, Sherwin J.; Han, Wei eLife, Vol. 5 https://doi.org/10.7554/eLife.11189	journal	January 2016
Update of the CHARMM All-Atom Additive Force Field for Lipids: Validation on Six Lipid Types Klauda, Jeffery B.; Venable, Richard M.; Freites, J. Alfredo The Journal of Physical Chemistry B, Vol. 114, Issue 23 https://doi.org/10.1021/jp101759q	journal	June 2010
Secondary Water Pore Formation for Proton Transport in a ClC Exchanger Revealed by an Atomistic Molecular-Dynamics Simulation Ko, Youn Jo; Jo, Won Ho Biophysical Journal, Vol. 98, Issue 10 https://doi.org/10.1016/j.bpj.2010.01.043	journal	May 2010
A CLC-type F-/H+ antiporter in ion-swapped conformations Last, Nicholas B.; Stockbridge, Randy B.; Wilson, Ashley E. Nature Structural & Molecular Biology, Vol. 25, Issue 7 https://doi.org/10.1038/s41594-018-0082-0	journal	June 2018
ClC-7 is a slowly voltage-gated 2Cl ⁻ /1H ⁺ -exchanger and requires Ostm1 for transport activity : Slowly voltage-gated 2Cl/H-exchange by ClC-7/Ostm1 Leisle, Lilia; Ludwig, Carmen F.; Wagner, Florian A. The EMBO Journal, Vol. 30, Issue 11 https://doi.org/10.1038/emboj.2011.137	journal	April 2011
Divergent Cl-and H+ pathways underlie transport coupling and gating in CLC exchangers and channels Leisle, Lilia; Xu, Yanyan; Fortea, Eva eLife, Vol. 9 https://doi.org/10.7554/eLife.51224	journal	April 2020
Intracellular Proton Access in a Cl−/H+ Antiporter Lim, Hyun-Ho; Shane, Tania; Miller, Christopher PLoS Biology, Vol. 10, Issue 12 https://doi.org/10.1371/journal.pbio.1001441	journal	December 2012
Intracellular Proton-Transfer Mutants in a CLC Cl ⁻ /H ⁺ Exchanger Lim, Hyun-Ho; Miller, Christopher The Journal of General Physiology, Vol. 133, Issue 2 https://doi.org/10.1085/jgp.200810112	journal	January 2009
Chronic renal failure in a boy with classic Bartter’s syndrome due to a novel mutation in CLCNKB coding for the chloride channel Lin, Chien-Ming; Tsai, Jeng-Daw; Lo, Yi-Fen European Journal of Pediatrics, Vol. 168, Issue 9 https://doi.org/10.1007/s00431-008-0883-y	journal	December 2008
Probing the Pore of ClC-0 by Substituted Cysteine Accessibility Method Using Methane Thiosulfonate Reagents Lin, Chia-Wei; Chen, Tsung-Yu The Journal of General Physiology, Vol. 122, Issue 2 https://doi.org/10.1085/jgp.200308845	journal	July 2003
The ClC-0 chloride channel is a 'broken' Cl−/H+ antiporter Lísal, Jiří; Maduke, Merritt Nature Structural & Molecular Biology, Vol. 15, Issue 8 https://doi.org/10.1038/nsmb.1466	journal	July 2008
A common molecular basis for three inherited kidney stone diseases Lloyd, Sarah E.; Pearce, Simon H. S.; Fisher, Simon E. Nature, Vol. 379, Issue 6564 https://doi.org/10.1038/379445a0	journal	February 1996
Ion-binding properties of the ClC chloride selectivity filter Lobet, Séverine; Dutzler, Raimund The EMBO Journal, Vol. 25, Issue 1 https://doi.org/10.1038/sj.emboj.7600909	journal	December 2005
ClC-5 mutations associated with Dent’s disease: a major role of the dimer interface Lourdel, Stéphane; Grand, Teddy; Burgos, Johanna Pflügers Archiv - European Journal of Physiology, Vol. 463, Issue 2 https://doi.org/10.1007/s00424-011-1052-0	journal	November 2011
Two physically distinct pores in the dimeric CIC-0 chloride channel Ludewig, Uwe; Pusch, Michael; Jentsch, Thomas J. Nature, Vol. 383, Issue 6598 https://doi.org/10.1038/383340a0	journal	September 1996
Relevance of hydrogen bond definitions in liquid water Matsumoto, Masakazu The Journal of Chemical Physics, Vol. 126, Issue 5 https://doi.org/10.1063/1.2431168	journal	February 2007
Side-Dependent Inhibition of a Prokaryotic ClC by DIDS Matulef, Kimberly; Maduke, Merritt Biophysical Journal, Vol. 89, Issue 3 https://doi.org/10.1529/biophysj.105.066522	journal	September 2005
The CLC ‘chloride channel’ family: revelations from prokaryotes (Review) Matulef, Kimberly; Maduke, Merritt Molecular Membrane Biology, Vol. 24, Issue 5-6 https://doi.org/10.1080/09687680701413874	journal	January 2007
Multiscale Kinetic Modeling Reveals an Ensemble of Cl ^– /H ⁺ Exchange Pathways in ClC-ec1 Antiporter Mayes, Heather B.; Lee, Sangyun; White, Andrew D. Journal of the American Chemical Society, Vol. 140, Issue 5 https://doi.org/10.1021/jacs.7b11463	journal	January 2018
Phaser crystallographic software McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D. Journal of Applied Crystallography, Vol. 40, Issue 4 https://doi.org/10.1107/S0021889807021206	journal	July 2007
Homodimeric architecture of a CIC-type chloride ion channel Middleton, Richard E.; Pheasant, Deborah J.; Miller, Christopher Nature, Vol. 383, Issue 6598 https://doi.org/10.1038/383337a0	journal	September 1996
ClC chloride channels viewed through a transporter lens Miller, Christopher Nature, Vol. 440, Issue 7083 https://doi.org/10.1038/nature04713	journal	March 2006
A provisional transport mechanism for a chloride channel-type Cl ⁻ /H ⁺ exchanger Miller, Christopher; Nguitragool, Wang Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 364, Issue 1514 https://doi.org/10.1098/rstb.2008.0138	journal	October 2008
Dimeric structure of single chloride channels from Torpedo electroplax. Miller, C.; White, M. M. Proceedings of the National Academy of Sciences, Vol. 81, Issue 9 https://doi.org/10.1073/pnas.81.9.2772	journal	May 1984
Conformational dynamics of the nucleotide binding domains and the power stroke of a heterodimeric ABC transporter Mishra, Smriti; Verhalen, Brandy; Stein, Richard A. eLife, Vol. 3 https://doi.org/10.7554/eLife.02740	journal	May 2014
Dipole Moment and Binding Energy of Water in Proteins from Crystallographic Analysis Morozenko, A.; Leontyev, I. V.; Stuchebrukhov, A. A. Journal of Chemical Theory and Computation, Vol. 10, Issue 10 https://doi.org/10.1021/ct500358r	journal	September 2014
Refinement of Macromolecular Structures by the Maximum-Likelihood Method Murshudov, G. N.; Vagin, A. A.; Dodson, E. J. Acta Crystallographica Section D Biological Crystallography, Vol. 53, Issue 3 https://doi.org/10.1107/S0907444996012255	journal	May 1997
CLC Cl-/H+ transporters constrained by covalent cross-linking Nguitragool, W.; Miller, C. Proceedings of the National Academy of Sciences, Vol. 104, Issue 52 https://doi.org/10.1073/pnas.0708639104	journal	December 2007
Tertiary Structure of Proteins. I. Representation and Computation of the Conformations Nishikawa, Ken; Ooi, Tatsuo; Isogai, Yoshinori Journal of the Physical Society of Japan, Vol. 32, Issue 5 https://doi.org/10.1143/JPSJ.32.1331	journal	May 1972
A unified formulation of the constant temperature molecular dynamics methods Nosé, Shuichi The Journal of Chemical Physics, Vol. 81, Issue 1 https://doi.org/10.1063/1.447334	journal	July 1984
PROPKA3: Consistent Treatment of Internal and Surface Residues in Empirical p K _a Predictions Olsson, Mats H. M.; Søndergaard, Chresten R.; Rostkowski, Michal Journal of Chemical Theory and Computation, Vol. 7, Issue 2 https://doi.org/10.1021/ct100578z	journal	December 2010
Insights into the ClC-4 Transport Mechanism from Studies of Zn2+ Inhibition Osteen, Jeremiah D.; Mindell, Joseph A. Biophysical Journal, Vol. 95, Issue 10 https://doi.org/10.1529/biophysj.108.137158	journal	November 2008
Structure and function of mitochondrial carriers - Role of the transmembrane helix P and G residues in the gating and transport mechanism Palmieri, Ferdinando; Pierri, Ciro Leonardo FEBS Letters, Vol. 584, Issue 9 https://doi.org/10.1016/j.febslet.2009.10.063	journal	October 2009
Mutation of external glutamate residue reveals a new intermediate transport state and anion binding site in a CLC Cl ⁻ /H ⁺ antiporter Park, Kunwoong; Lee, Byoung-Cheol; Lim, Hyun-Ho Proceedings of the National Academy of Sciences, Vol. 116, Issue 35 https://doi.org/10.1073/pnas.1901822116	journal	August 2019
Structure of the CLC-1 chloride channel from Homo sapiens Park, Eunyong; MacKinnon, Roderick eLife, Vol. 7 https://doi.org/10.7554/eLife.36629	journal	May 2018
Scalable molecular dynamics with NAMD Phillips, James C.; Braun, Rosemary; Wang, Wei Journal of Computational Chemistry, Vol. 26, Issue 16, p. 1781-1802 https://doi.org/10.1002/jcc.20289	journal	January 2005
Surprises from an Unusual CLC Homolog Phillips, Sabrina; Brammer, Ashley E.; Rodriguez, Luis Biophysical Journal, Vol. 103, Issue 9 https://doi.org/10.1016/j.bpj.2012.08.063	journal	November 2012
Basis of substrate binding and conservation of selectivity in the CLC family of channels and transporters Picollo, Alessandra; Malvezzi, Mattia; Houtman, Jon C. D. Nature Structural & Molecular Biology, Vol. 16, Issue 12 https://doi.org/10.1038/nsmb.1704	journal	November 2009
Synergistic substrate binding determines the stoichiometry of transport of a prokaryotic H+/Cl− exchanger Picollo, Alessandra; Xu, Yanyan; Johner, Niklaus Nature Structural & Molecular Biology, Vol. 19, Issue 5 https://doi.org/10.1038/nsmb.2277	journal	April 2012
Myotonia caused by mutations in the muscle chloride channel geneCLCN1 Pusch, Michael Human Mutation, Vol. 19, Issue 4 https://doi.org/10.1002/humu.10063	journal	March 2002
The Alternating-Access Mechanism of MFS Transporters Arises from Inverted-Topology Repeats Radestock, Sebastian; Forrest, Lucy R. Journal of Molecular Biology, Vol. 407, Issue 5 https://doi.org/10.1016/j.jmb.2011.02.008	journal	April 2011
Design, function and structure of a monomeric ClC transporter Robertson, Janice L.; Kolmakova-Partensky, Ludmila; Miller, Christopher Nature, Vol. 468, Issue 7325 https://doi.org/10.1038/nature09556	journal	November 2010
Graphical analysis of pH-dependent properties of proteins predicted using PROPKA Rostkowski, Michał; Olsson, Mats HM; Søndergaard, Chresten R. BMC Structural Biology, Vol. 11, Issue 1 https://doi.org/10.1186/1472-6807-11-6	journal	January 2011
The Muscle Chloride Channel ClC-1 Has a Double-Barreled Appearance that Is Differentially Affected in Dominant and Recessive Myotonia Saviane, Chiara; Conti, Franco; Pusch, Michael Journal of General Physiology, Vol. 113, Issue 3 https://doi.org/10.1085/jgp.113.3.457	journal	March 1999
HOLE: A program for the analysis of the pore dimensions of ion channel structural models Smart, Oliver S.; Neduvelil, Joseph G.; Wang, Xiaonan Journal of Molecular Graphics, Vol. 14, Issue 6 https://doi.org/10.1016/S0263-7855(97)00009-X	journal	December 1996
Characterization of Dent's disease mutations of CLC-5 reveals a correlation between functional and cell biological consequences and protein structure Smith, Andrew J.; Reed, Anita A. C.; Loh, Nellie Y. American Journal of Physiology-Renal Physiology, Vol. 296, Issue 2 https://doi.org/10.1152/ajprenal.90526.2008	journal	February 2009
Disruption of ClC-3, a Chloride Channel Expressed on Synaptic Vesicles, Leads to a Loss of the Hippocampus Stobrawa, Sandra M.; Breiderhoff, Tilman; Takamori, Shigeo Neuron, Vol. 29, Issue 1 https://doi.org/10.1016/S0896-6273(01)00189-1	journal	January 2001
Fluoride resistance and transport by riboswitch-controlled CLC antiporters Stockbridge, Randy B.; Lim, Hyun-Ho; Otten, Renee Proceedings of the National Academy of Sciences, Vol. 109, Issue 38 https://doi.org/10.1073/pnas.1210896109	journal	September 2012
Mechanism of Free Energy Coupling in Active Transport Tanford, C. Annual Review of Biochemistry, Vol. 52, Issue 1 https://doi.org/10.1146/annurev.bi.52.070183.002115	journal	June 1983
Physiology and Pathophysiology of CLC-1: Mechanisms of a Chloride Channel Disease, Myotonia Tang, Chih-Yung; Chen, Tsung-Yu Journal of Biomedicine and Biotechnology, Vol. 2011 https://doi.org/10.1155/2011/685328	journal	January 2011
Exome sequencing reveals new causal mutations in children with epileptic encephalopathies Veeramah, Krishna R.; Johnstone, Laurel; Karafet, Tatiana M. Epilepsia, Vol. 54, Issue 7 https://doi.org/10.1111/epi.12201	journal	May 2013
Uncoupling and Turnover in a Cl ⁻ /H ⁺ Exchange Transporter Walden, Michael; Accardi, Alessio; Wu, Fang The Journal of General Physiology, Vol. 129, Issue 4 https://doi.org/10.1085/jgp.200709756	journal	March 2007
Modulating the Chemical Transport Properties of a Transmembrane Antiporter via Alternative Anion Flux Wang, Zhi; Swanson, Jessica M. J.; Voth, Gregory A. Journal of the American Chemical Society, Vol. 140, Issue 48 https://doi.org/10.1021/jacs.8b07614	journal	November 2018
Proton Transport Pathway in the ClC Cl−/H+ Antiporter Wang, Dong; Voth, Gregory A. Biophysical Journal, Vol. 97, Issue 1 https://doi.org/10.1016/j.bpj.2009.04.038	journal	July 2009
Overview of the CCP 4 suite and current developments Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D. Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S0907444910045749	journal	March 2011
Identification of Functionally Important Regions of the Muscular Chloride Channel ClC-1 by Analysis of Recessive and Dominant Myotonic Mutations Wollnik, B.; Kubisch, C.; Steinmeyer, K. Human Molecular Genetics, Vol. 6, Issue 5 https://doi.org/10.1093/hmg/6.5.805	journal	May 1997
CHARMM-GUI Membrane Builder toward realistic biological membrane simulations Wu, Emilia L.; Cheng, Xi; Jo, Sunhwan Journal of Computational Chemistry, Vol. 35, Issue 27 https://doi.org/10.1002/jcc.23702	journal	August 2014
Determinants of Anion-Proton Coupling in Mammalian Endosomal CLC Proteins Zdebik, Anselm A.; Zifarelli, Giovanni; Bergsdorf, Eun-Yeong Journal of Biological Chemistry, Vol. 283, Issue 7 https://doi.org/10.1074/jbc.M708368200	journal	December 2007
Mechanism of Inverted Activation of ClC-1 Channels Caused by a Novel Myotonia Congenita Mutation Zhang, Jie; Sanguinetti, Michael C.; Kwiecinski, Hubert Journal of Biological Chemistry, Vol. 275, Issue 4 https://doi.org/10.1074/jbc.275.4.2999	journal	January 2000
Hydrophilicity of cavities in proteins Zhang, Li; Hermans, Jan Proteins: Structure, Function, and Genetics, Vol. 24, Issue 4 https://doi.org/10.1002/(SICI)1097-0134(199604)24:4<433::AID-PROT3>3.0.CO;2-F	journal	April 1996

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