Phenotyping stomatal closure by thermal imaging for GWAS and TWAS of water use efficiency-related genes

Pignon, Charles P.; Fernandes, Samuel B.; Valluru, Ravi; Bandillo, Nonoy; Lozano, Roberto; Buckler, Edward; Gore, Michael A.; Long, Stephen P.; Brown, Patrick J.; Leakey, Andrew D. B.

doi:10.1093/plphys/kiab395

Phenotyping stomatal closure by thermal imaging for GWAS and TWAS of water use efficiency-related genes

Journal Article · Mon Aug 16 00:00:00 EDT 2021 · Plant Physiology (Bethesda)

DOI:https://doi.org/10.1093/plphys/kiab395· OSTI ID:1834264

^[1]; ^[2]; ^[3]; ^[4]; Lozano, Roberto ^[5]; ^[6]; ^[5]; ^[7]; ^[2]; ^[8]

Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA, Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA, Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA
Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA, Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA
Institute for Genomic Diversity, Cornell University, Ithaca, New York 14853, USA, Lincoln Institute for Agri-Food Technology, University of Lincoln, Lincoln LN1 3QE, UK
Institute for Genomic Diversity, Cornell University, Ithaca, New York 14853, USA, Department of Plant Sciences, North Dakota State University, Fargo, North Dakota 58105, USA
Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, Ithaca, New York 14853, USA
Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, Ithaca, New York 14853, USA, United States Department of Agriculture, Agricultural Research Service (USDA-ARS) R.W. Holley Center for Agriculture and Health, Ithaca, New York 14853, USA
Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA, Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA, Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA, Lancaster Environment Centre, University of Lancaster, Lancaster LA1 1YX, UK
Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA, Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA, Institute for Genomic Diversity, Cornell University, Ithaca, New York 14853, USA

Abstract

Stomata allow CO2 uptake by leaves for photosynthetic assimilation at the cost of water vapor loss to the atmosphere. The opening and closing of stomata in response to fluctuations in light intensity regulate CO2 and water fluxes and are essential for maintaining water-use efficiency (WUE). However, a little is known about the genetic basis for natural variation in stomatal movement, especially in C4 crops. This is partly because the stomatal response to a change in light intensity is difficult to measure at the scale required for association studies. Here, we used high-throughput thermal imaging to bypass the phenotyping bottleneck and assess 10 traits describing stomatal conductance (gs) before, during and after a stepwise decrease in light intensity for a diversity panel of 659 sorghum (Sorghum bicolor) accessions. Results from thermal imaging significantly correlated with photosynthetic gas exchange measurements. gs traits varied substantially across the population and were moderately heritable (h2 up to 0.72). An integrated genome-wide and transcriptome-wide association study identified candidate genes putatively driving variation in stomatal conductance traits. Of the 239 unique candidate genes identified with the greatest confidence, 77 were putative orthologs of Arabidopsis (Arabidopsis thaliana) genes related to functions implicated in WUE, including stomatal opening/closing (24 genes), stomatal/epidermal cell development (35 genes), leaf/vasculature development (12 genes), or chlorophyll metabolism/photosynthesis (8 genes). These findings demonstrate an approach to finding genotype-to-phenotype relationships for a challenging trait as well as candidate genes for further investigation of the genetic basis of WUE in a model C4 grass for bioenergy, food, and forage production.

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Research Organization:: Donald Danforth Plant Science Center, St. Louis, MO (United States); University of Illinois at Urbana-Champaign, IL (United States)

Sponsoring Organization:: USDOE Advanced Research Projects Agency - Energy (ARPA-E); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AR0000661; SC0018277

OSTI ID:: 1834264

Alternate ID(s):: OSTI ID: 2305733
OSTI ID: 1904828

Journal Information:: Plant Physiology (Bethesda), Journal Name: Plant Physiology (Bethesda) Journal Issue: 4 Vol. 187; ISSN 0032-0889

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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