Genome-wide association analysis of the strength of the MAMP-elicited defense response and resistance to target leaf spot in sorghum

Samira, Rozalynne; Kimball, Jennifer A.; Samayoa, Luis Fernando; Holland, James B.; Jamann, Tiffany M.; Brown, Patrick J.; Stacey, Gary; Balint-Kurti, Peter J.

doi:10.1038/s41598-020-77684-w

Title: Genome-wide association analysis of the strength of the MAMP-elicited defense response and resistance to target leaf spot in sorghum

Journal Article · Mon Nov 30 00:00:00 EST 2020 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-020-77684-w· OSTI ID:1833747

Samira, Rozalynne; Kimball, Jennifer A.; Samayoa, Luis Fernando; Holland, James B.; Jamann, Tiffany M.; Brown, Patrick J.; Stacey, Gary; Balint-Kurti, Peter J.

Abstract Plants have the capacity to respond to conserved molecular features known as microbe-associated molecular patterns (MAMPs). The goal of this work was to assess variation in the MAMP response in sorghum, to map loci associated with this variation, and to investigate possible connections with variation in quantitative disease resistance. Using an assay that measures the production of reactive oxygen species, we assessed variation in the MAMP response in a sorghum association mapping population known as the sorghum conversion population (SCP). We identified consistent variation for the response to chitin and flg22—an epitope of flagellin. We identified two SNP loci associated with variation in the flg22 response and one with the chitin response. We also assessed resistance to Target Leaf Spot (TLS) disease caused by the necrotrophic fungus Bipolaris cookei in the SCP. We identified one strong association on chromosome 5 near a previously characterized disease resistance gene. A moderately significant correlation was observed between stronger flg22 response and lower TLS resistance. Possible reasons for this are discussed.

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Research Organization:: North Carolina State University, Raleigh, NC (United States); Univ. of Illinois at Urbana-Champaign, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0014116; SC0019189

OSTI ID:: 1833747

Alternate ID(s):: OSTI ID: 1816576

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Vol. 10 Journal Issue: 1; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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