Casposase structure and the mechanistic link between DNA transposition and spacer acquisition by CRISPR-Cas

Hickman, Alison B.; Kailasan, Shweta; Genzor, Pavol; Haase, Astrid D.; Dyda, Fred

doi:10.7554/elife.50004

Title: Casposase structure and the mechanistic link between DNA transposition and spacer acquisition by CRISPR-Cas

Journal Article · Wed Jan 08 00:00:00 EST 2020 · eLife

DOI:https://doi.org/10.7554/elife.50004· OSTI ID:1833697

^[1];

^[1]; Genzor, Pavol ^[2]; Haase, Astrid D. ^[2];

^[1]

National Institutes of Health (NIH), Bethesda, MD (United States). Lab. of Molecular Biology. National Inst. of Diabetes and Digestive and Kidney Diseases
National Institutes of Health (NIH), Bethesda, MD (United States). Lab. of Cell and Molecular Biology. National Inst. of Diabetes and Digestive and Kidney Diseases

Key to CRISPR-Cas adaptive immunity is maintaining an ongoing record of invading nucleic acids, a process carried out by the Cas1-Cas2 complex that integrates short segments of foreign genetic material (spacers) into the CRISPR locus. It is hypothesized that Cas1 evolved from casposases, a novel class of transposases. We show here that the Methanosarcina mazei casposase can integrate varied forms of the casposon end in vitro, and recapitulates several properties of CRISPR-Cas integrases including site-specificity. The X-ray structure of the casposase bound to DNA representing the product of integration reveals a tetramer with target DNA bound snugly between two dimers in which single-stranded casposon end binding resembles that of spacer 3'-overhangs. The differences between transposase and CRISPR-Cas integrase are largely architectural, and it appears that evolutionary change involved changes in protein-protein interactions to favor Cas2 binding over tetramerization; this in turn led to preferred integration of single spacers over two transposon ends.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI ID:: 1833697

Journal Information:: eLife, Vol. 9; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: ENGLISH

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