Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies

Cottrell, Christopher A.; Manne, Kartik; Kong, Rui; Wang, Shuishu; Zhou, Tongqing; Chuang, Gwo-Yu; Edwards, Robert J.; Henderson, Rory; Janowska, Katarzyna; Kopp, Megan; Lin, Bob C.; Louder, Mark K.; Olia, Adam S.; Rawi, Reda; Shen, Chen-Hsiang; Taft, Justin D.; Torres, Jonathan L.; Wu, Nelson R.; Zhang, Baoshan; Doria-Rose, Nicole A.; Cohen, Myron S.; Haynes, Barton F.; Shapiro, Lawrence; Ward, Andrew B.; Acharya, Priyamvada; Mascola, John R.; Kwong, Peter D.

doi:10.1016/j.celrep.2021.109922

Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies

Journal Article · Tue Nov 02 00:00:00 EDT 2021 · Cell Reports

DOI:https://doi.org/10.1016/j.celrep.2021.109922· OSTI ID:1829863

^[1]; ^[2]; Kong, Rui ^[3]; ^[3]; ^[3]; Chuang, Gwo-Yu ^[3]; Edwards, Robert J. ^[2]; Henderson, Rory ^[2]; Janowska, Katarzyna ^[2]; ^[2]; Lin, Bob C. ^[3]; Louder, Mark K. ^[3]; ^[3]; Rawi, Reda ^[3]; Shen, Chen-Hsiang ^[3]; Taft, Justin D. ^[3]; ^[1]; ^[1]; Zhang, Baoshan ^[3]; Doria-Rose, Nicole A. ^[3] more »

The Scripps Research Inst., La Jolla, CA (United States)
Duke Univ., Durham, NC (United States)
National Institutes of Health (NIH), Bethesda, MD (United States)
Univ. of North Carolina, Chapel Hill, NC (United States)
National Institutes of Health (NIH), Bethesda, MD (United States); Columbia Univ., New York, NY (United States)
Duke Univ., Durham, NC (United States); National Institutes of Health (NIH), Bethesda, MD (United States)

Recognition of N-linked glycan at residue N276 (glycan276) at the periphery of the CD4-binding site (CD4bs) on the HIV-envelope trimer is a formidable challenge for many CD4bs-directed antibodies. To understand how this glycan can be recognized, here we isolate two lineages of glycan276-dependent CD4bs antibodies. Antibody CH540-VRC40.01 (named for donor-lineage.clone) neutralizes 81% of a panel of 208 diverse strains, while antibody CH314-VRC33.01 neutralizes 45%. Cryo-electron microscopy (cryo-EM) structures of these two antibodies and 179NC75, a previously identified glycan276-dependent CD4bs antibody, in complex with HIV-envelope trimer reveal substantially different modes of glycan276 recognition. Despite these differences, binding of glycan276-dependent antibodies maintains a glycan276 conformation similar to that observed in the absence of glycan276-binding antibodies. By contrast, glycan276-independent CD4bs antibodies, such as VRC01, displace glycan276 upon binding. These results provide a foundation for understanding antibody recognition of glycan276 and suggest its presence may be crucial for priming immunogens seeking to initiate broad CD4bs recognition.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: National Institute of Allergy and Infectious Diseases (NIAID); National Institutes of Health (NIH); National Science Foundation (NSF); Scripps CHAVD; USDOE Office of Science (SC), Basic Energy Sciences (BES); Vaccine Research Center

Grant/Contract Number:: W-31109-ENG-38

OSTI ID:: 1829863

Journal Information:: Cell Reports, Journal Name: Cell Reports Journal Issue: 5 Vol. 37; ISSN 2211-1247

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

References (81)

Regulation of immunoglobulin light chain gene rearrangements during early B cell development in the human Bräuninger, Andreas; Goossens, Tina; Rajewsky, Klaus European Journal of Immunology, Vol. 31, Issue 12 https://doi.org/10.1002/1521-4141(200112)31:12<3631::AID-IMMU3631>3.0.CO;2-L	journal	December 2001
UCSF Chimera?A visualization system for exploratory research and analysis Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C. Journal of Computational Chemistry, Vol. 25, Issue 13 https://doi.org/10.1002/jcc.20084	journal	January 2004
MolProbity: More and better reference data for improved all-atom structure validation: PROTEIN SCIENCE.ORG Williams, Christopher J.; Headd, Jeffrey J.; Moriarty, Nigel W. Protein Science, Vol. 27, Issue 1 https://doi.org/10.1002/pro.3330	journal	November 2017
Current developments in Coot for macromolecular model building of Electron Cryo‐microscopy and Crystallographic Data Casañal, Ana; Lohkamp, Bernhard; Emsley, Paul Protein Science, Vol. 29, Issue 4 https://doi.org/10.1002/pro.3791	journal	March 2020
UCSF ChimeraX : Structure visualization for researchers, educators, and developers Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C. Protein Science, Vol. 30, Issue 1 https://doi.org/10.1002/pro.3943	journal	October 2020
[20] Processing of X-ray diffraction data collected in oscillation mode Otwinowski, Zbyszek; Minor, Wladek Macromolecular Crystallography Part A, 307-326 https://doi.org/10.1016/S0076-6879(97)76066-X	book	January 1997
Structural Repertoire of HIV-1-Neutralizing Antibodies Targeting the CD4 Supersite in 14 Donors Zhou, Tongqing; Lynch, Rebecca M.; Chen, Lei Cell, Vol. 161, Issue 6 https://doi.org/10.1016/j.cell.2015.05.007	journal	June 2015
Maturation Pathway from Germline to Broad HIV-1 Neutralizer of a CD4-Mimic Antibody Bonsignori, Mattia; Zhou, Tongqing; Sheng, Zizhang Cell, Vol. 165, Issue 2 https://doi.org/10.1016/j.cell.2016.02.022	journal	April 2016
Induction of HIV Neutralizing Antibody Lineages in Mice with Diverse Precursor Repertoires Tian, Ming; Cheng, Cheng; Chen, Xuejun Cell, Vol. 166, Issue 6 https://doi.org/10.1016/j.cell.2016.07.029	journal	September 2016
Tailored Immunogens Direct Affinity Maturation toward HIV Neutralizing Antibodies Briney, Bryan; Sok, Devin; Jardine, Joseph G. Cell, Vol. 166, Issue 6 https://doi.org/10.1016/j.cell.2016.08.005	journal	September 2016
Restriction of HIV-1 Escape by a Highly Broad and Potent Neutralizing Antibody Schommers, Philipp; Gruell, Henning; Abernathy, Morgan E. Cell, Vol. 180, Issue 3 https://doi.org/10.1016/j.cell.2020.01.010	journal	February 2020
Antibody 8ANC195 Reveals a Site of Broad Vulnerability on the HIV-1 Envelope Spike Scharf, Louise; Scheid, Johannes F.; Lee, Jeong Hyun Cell Reports, Vol. 7, Issue 3 https://doi.org/10.1016/j.celrep.2014.04.001	journal	May 2014
Direct Probing of Germinal Center Responses Reveals Immunological Features and Bottlenecks for Neutralizing Antibody Responses to HIV Env Trimer Havenar-Daughton, Colin; Carnathan, Diane G.; Torrents de la Peña, Alba Cell Reports, Vol. 17, Issue 9 https://doi.org/10.1016/j.celrep.2016.10.085	journal	November 2016
Quantification of the Impact of the HIV-1-Glycan Shield on Antibody Elicitation Zhou, Tongqing; Doria-Rose, Nicole A.; Cheng, Cheng Cell Reports, Vol. 19, Issue 4 https://doi.org/10.1016/j.celrep.2017.04.013	journal	April 2017
Improving the Immunogenicity of Native-like HIV-1 Envelope Trimers by Hyperstabilization Torrents de la Peña, Alba; Julien, Jean-Philippe; de Taeye, Steven W. Cell Reports, Vol. 20, Issue 8 https://doi.org/10.1016/j.celrep.2017.07.077	journal	August 2017
Surface-Matrix Screening Identifies Semi-specific Interactions that Improve Potency of a Near Pan-reactive HIV-1-Neutralizing Antibody Kwon, Young D.; Chuang, Gwo-Yu; Zhang, Baoshan Cell Reports, Vol. 22, Issue 7 https://doi.org/10.1016/j.celrep.2018.01.023	journal	February 2018
Overcoming Steric Restrictions of VRC01 HIV-1 Neutralizing Antibodies through Immunization Parks, K. Rachael; MacCamy, Anna J.; Trichka, Josephine Cell Reports, Vol. 29, Issue 10 https://doi.org/10.1016/j.celrep.2019.10.071	journal	December 2019
Structure-Based Design with Tag-Based Purification and In-Process Biotinylation Enable Streamlined Development of SARS-CoV-2 Spike Molecular Probes Zhou, Tongqing; Teng, I-Ting; Olia, Adam S. Cell Reports, Vol. 33, Issue 4 https://doi.org/10.1016/j.celrep.2020.108322	journal	October 2020
Automated Design by Structure-Based Stabilization and Consensus Repair to Achieve Prefusion-Closed Envelope Trimers in a Wide Variety of HIV Strains Rawi, Reda; Rutten, Lucy; Lai, Yen-Ting Cell Reports, Vol. 33, Issue 8 https://doi.org/10.1016/j.celrep.2020.108432	journal	November 2020
Multidonor Analysis Reveals Structural Elements, Genetic Determinants, and Maturation Pathway for HIV-1 Neutralization by VRC01-Class Antibodies Zhou, Tongqing; Zhu, Jiang; Wu, Xueling Immunity, Vol. 39, Issue 2 https://doi.org/10.1016/j.immuni.2013.04.012	journal	August 2013
Key gp120 Glycans Pose Roadblocks to the Rapid Development of VRC01-Class Antibodies in an HIV-1-Infected Chinese Donor Kong, Leopold; Ju, Bin; Chen, Yajing Immunity, Vol. 44, Issue 4 https://doi.org/10.1016/j.immuni.2016.03.006	journal	April 2016
Identification of a CD4-Binding-Site Antibody to HIV that Evolved Near-Pan Neutralization Breadth Huang, Jinghe; Kang, Byong H.; Ishida, Elise Immunity, Vol. 45, Issue 5 https://doi.org/10.1016/j.immuni.2016.10.027	journal	November 2016
Precursor Frequency and Affinity Determine B Cell Competitive Fitness in Germinal Centers, Tested with Germline-Targeting HIV Vaccine Immunogens Abbott, Robert K.; Lee, Jeong Hyun; Menis, Sergey Immunity, Vol. 48, Issue 1 https://doi.org/10.1016/j.immuni.2017.11.023	journal	January 2018
A Neutralizing Antibody Recognizing Primarily N-Linked Glycan Targets the Silent Face of the HIV Envelope Zhou, Tongqing; Zheng, Anqi; Baxa, Ulrich Immunity, Vol. 48, Issue 3 https://doi.org/10.1016/j.immuni.2018.02.013	journal	March 2018
Inference of the HIV-1 VRC01 Antibody Lineage Unmutated Common Ancestor Reveals Alternative Pathways to Overcome a Key Glycan Barrier Bonsignori, Mattia; Scott, Eric; Wiehe, Kevin Immunity, Vol. 49, Issue 6 https://doi.org/10.1016/j.immuni.2018.10.015	journal	December 2018
Rapid and Focused Maturation of a VRC01-Class HIV Broadly Neutralizing Antibody Lineage Involves Both Binding and Accommodation of the N276-Glycan Umotoy, Jeffrey; Bagaya, Bernard S.; Joyce, Collin Immunity, Vol. 51, Issue 1 https://doi.org/10.1016/j.immuni.2019.06.004	journal	July 2019
Vaccination induces maturation in a mouse model of diverse unmutated VRC01-class precursors to HIV-neutralizing antibodies with >50% breadth Chen, Xuejun; Zhou, Tongqing; Schmidt, Stephen D. Immunity, Vol. 54, Issue 2 https://doi.org/10.1016/j.immuni.2020.12.014	journal	February 2021
Inference of Macromolecular Assemblies from Crystalline State Krissinel, Evgeny; Henrick, Kim Journal of Molecular Biology, Vol. 372, Issue 3 https://doi.org/10.1016/j.jmb.2007.05.022	journal	September 2007
Refinement of Protein Structures into Low-Resolution Density Maps Using Rosetta DiMaio, Frank; Tyka, Michael D.; Baker, Matthew L. Journal of Molecular Biology, Vol. 392, Issue 1 https://doi.org/10.1016/j.jmb.2009.07.008	journal	September 2009
Automated molecular microscopy: The new Leginon system Suloway, Christian; Pulokas, James; Fellmann, Denis Journal of Structural Biology, Vol. 151, Issue 1 https://doi.org/10.1016/j.jsb.2005.03.010	journal	July 2005
Gctf: Real-time CTF determination and correction Zhang, Kai Journal of Structural Biology, Vol. 193, Issue 1 https://doi.org/10.1016/j.jsb.2015.11.003	journal	January 2016
Structural Survey of Broadly Neutralizing Antibodies Targeting the HIV-1 Env Trimer Delineates Epitope Categories and Characteristics of Recognition Chuang, Gwo-Yu; Zhou, Jing; Acharya, Priyamvada Structure, Vol. 27, Issue 1 https://doi.org/10.1016/j.str.2018.10.007	journal	January 2019
A non-affinity purification process for GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C for clinical evaluation Gulla, Krishana; Cibelli, Nicole; Cooper, Jonathan W. Vaccine, Vol. 39, Issue 25 https://doi.org/10.1016/j.vaccine.2021.04.063	journal	June 2021
Glycosylation Site-Specific Analysis of HIV Envelope Proteins (JR-FL and CON-S) Reveals Major Differences in Glycosylation Site Occupancy, Glycoform Profiles, and Antigenic Epitopesʼ Accessibility Go, Eden P.; Irungu, Janet; Zhang, Ying Journal of Proteome Research, Vol. 7, Issue 4 https://doi.org/10.1021/pr7006957	journal	April 2008
Structural definition of a conserved neutralization epitope on HIV-1 gp120 Zhou, Tongqing; Xu, Ling; Dey, Barna Nature, Vol. 445, Issue 7129 https://doi.org/10.1038/nature05580	journal	February 2007
Co-evolution of a broadly neutralizing HIV-1 antibody and founder virus Liao, Hua-Xin; Lynch, Rebecca; Zhou, Tongqing Nature, Vol. 496, Issue 7446 https://doi.org/10.1038/nature12053	journal	April 2013
Structure and immune recognition of trimeric pre-fusion HIV-1 Env Pancera, Marie; Zhou, Tongqing; Druz, Aliaksandr Nature, Vol. 514, Issue 7523 https://doi.org/10.1038/nature13808	journal	October 2014
EMRinger: side chain–directed model and map validation for 3D cryo-electron microscopy Barad, Benjamin A.; Echols, Nathaniel; Wang, Ray Yu-Ruei Nature Methods, Vol. 12, Issue 10 https://doi.org/10.1038/nmeth.3541	journal	August 2015
cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination Punjani, Ali; Rubinstein, John L.; Fleet, David J. Nature Methods, Vol. 14, Issue 3 https://doi.org/10.1038/nmeth.4169	journal	February 2017
MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy Zheng, Shawn Q.; Palovcak, Eugene; Armache, Jean-Paul Nature Methods, Vol. 14, Issue 4 https://doi.org/10.1038/nmeth.4193	journal	February 2017
Modeling and docking of antibody structures with Rosetta Weitzner, Brian D.; Jeliazkov, Jeliazko R.; Lyskov, Sergey Nature Protocols, Vol. 12, Issue 2 https://doi.org/10.1038/nprot.2016.180	journal	January 2017
Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env Do Kwon, Young; Pancera, Marie; Acharya, Priyamvada Nature Structural & Molecular Biology, Vol. 22, Issue 7 https://doi.org/10.1038/nsmb.3051	journal	June 2015
Privateer: software for the conformational validation of carbohydrate structures Agirre, Jon; Iglesias-Fernández, Javier; Rovira, Carme Nature Structural & Molecular Biology, Vol. 22, Issue 11 https://doi.org/10.1038/nsmb.3115	journal	November 2015
Natively glycosylated HIV-1 Env structure reveals new mode for antibody recognition of the CD4-binding site Gristick, Harry B.; von Boehmer, Lotta; West Jr, Anthony P. Nature Structural & Molecular Biology, Vol. 23, Issue 10 https://doi.org/10.1038/nsmb.3291	journal	September 2016
Targeting HIV Env immunogens to B cell follicles in nonhuman primates through immune complex or protein nanoparticle formulations Martin, Jacob T.; Cottrell, Christopher A.; Antanasijevic, Aleksandar npj Vaccines, Vol. 5, Issue 1 https://doi.org/10.1038/s41541-020-00223-1	journal	August 2020
Improvement of cryo-EM maps by density modification Terwilliger, Thomas C.; Ludtke, Steven J.; Read, Randy J. Nature Methods, Vol. 17, Issue 9 https://doi.org/10.1038/s41592-020-0914-9	journal	August 2020
Structural basis for germ-line gene usage of a potent class of antibodies targeting the CD4-binding site of HIV-1 gp120 West, Anthony P.; Diskin, Ron; Nussenzweig, Michel C. Proceedings of the National Academy of Sciences, Vol. 109, Issue 30 https://doi.org/10.1073/pnas.1208984109	journal	June 2012
De novo identification of VRC01 class HIV-1-neutralizing antibodies by next-generation sequencing of B-cell transcripts Zhu, J.; Wu, X.; Zhang, B. Proceedings of the National Academy of Sciences, Vol. 110, Issue 43 https://doi.org/10.1073/pnas.1306262110	journal	October 2013
Engineering HIV envelope protein to activate germline B cell receptors of broadly neutralizing anti-CD4 binding site antibodies McGuire, Andrew T.; Hoot, Sam; Dreyer, Anita M. The Journal of Experimental Medicine, Vol. 210, Issue 4 https://doi.org/10.1084/jem.20122824	journal	March 2013
Design and crystal structure of a native-like HIV-1 envelope trimer that engages multiple broadly neutralizing antibody precursors in vivo Medina-Ramírez, Max; Garces, Fernando; Escolano, Amelia The Journal of Experimental Medicine, Vol. 214, Issue 9 https://doi.org/10.1084/jem.20161160	journal	August 2017
OLGA: fast computation of generation probabilities of B- and T-cell receptor amino acid sequences and motifs Sethna, Zachary; Elhanati, Yuval; Callan, Curtis G. Bioinformatics, Vol. 35, Issue 17 https://doi.org/10.1093/bioinformatics/btz035	journal	January 2019
IgBLAST: an immunoglobulin variable domain sequence analysis tool Ye, Jian; Ma, Ning; Madden, Thomas L. Nucleic Acids Research, Vol. 41, Issue W1 https://doi.org/10.1093/nar/gkt382	journal	May 2013
Phaser crystallographic software McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D. Journal of Applied Crystallography, Vol. 40, Issue 4 https://doi.org/10.1107/S0021889807021206	journal	July 2007
Coot model-building tools for molecular graphics Emsley, Paul; Cowtan, Kevin Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132 https://doi.org/10.1107/S0907444904019158	journal	November 2004
PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
ISOLDE : a physically realistic environment for model building into low-resolution electron-density maps Croll, Tristan Ian Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 6 https://doi.org/10.1107/S2059798318002425	journal	April 2018
Building and rebuilding N-glycans in protein structure models van Beusekom, Bart; Wezel, Natasja; Hekkelman, Maarten L. Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 4 https://doi.org/10.1107/S2059798319003875	journal	April 2019
Rational Design of Envelope Identifies Broadly Neutralizing Human Monoclonal Antibodies to HIV-1 Wu, X.; Yang, Z. -Y.; Li, Y. Science, Vol. 329, Issue 5993 https://doi.org/10.1126/science.1187659	journal	July 2010
Structural Basis for Broad and Potent Neutralization of HIV-1 by Antibody VRC01 Zhou, T.; Georgiev, I.; Wu, X. Science, Vol. 329, Issue 5993 https://doi.org/10.1126/science.1192819	journal	July 2010
Sequence and Structural Convergence of Broad and Potent HIV Antibodies That Mimic CD4 Binding Scheid, J. F.; Mouquet, H.; Ueberheide, B. Science, Vol. 333, Issue 6049 https://doi.org/10.1126/science.1207227	journal	July 2011
Focused Evolution of HIV-1 Neutralizing Antibodies Revealed by Structures and Deep Sequencing Wu, X.; Zhou, T.; Zhu, J. Science, Vol. 333, Issue 6049 https://doi.org/10.1126/science.1207532	journal	August 2011
Delineating Antibody Recognition in Polyclonal Sera from Patterns of HIV-1 Isolate Neutralization Georgiev, I. S.; Doria-Rose, N. A.; Zhou, T. Science, Vol. 340, Issue 6133 https://doi.org/10.1126/science.1233989	journal	May 2013
Priming a broadly neutralizing antibody response to HIV-1 using a germline-targeting immunogen Jardine, J. G.; Ota, T.; Sok, D. Science, Vol. 349, Issue 6244 https://doi.org/10.1126/science.aac5894	journal	June 2015
HIV-1 broadly neutralizing antibody precursor B cells revealed by germline-targeting immunogen Jardine, J. G.; Kulp, D. W.; Havenar-Daughton, C. Science, Vol. 351, Issue 6280 https://doi.org/10.1126/science.aad9195	journal	March 2016
The human naive B cell repertoire contains distinct subclasses for a germline-targeting HIV-1 vaccine immunogen Havenar-Daughton, Colin; Sarkar, Anita; Kulp, Daniel W. Science Translational Medicine, Vol. 10, Issue 448 https://doi.org/10.1126/scitranslmed.aat0381	journal	July 2018
Immune Evasion of Porcine Reproductive and Respiratory Syndrome Virus through Glycan Shielding Involves both Glycoprotein 5 as Well as Glycoprotein 3 Vu, Hiep L. X.; Kwon, Byungjoon; Yoon, Kyoung-Jin Journal of Virology, Vol. 85, Issue 11 https://doi.org/10.1128/JVI.00189-11	journal	June 2011
Residue-Level Prediction of HIV-1 Antibody Epitopes Based on Neutralization of Diverse Viral Strains Chuang, G. -Y.; Acharya, P.; Schmidt, S. D. Journal of Virology, Vol. 87, Issue 18 https://doi.org/10.1128/JVI.00984-13	journal	July 2013
Structure of an N276-Dependent HIV-1 Neutralizing Antibody Targeting a Rare V5 Glycan Hole Adjacent to the CD4 Binding Site Wibmer, Constantinos Kurt; Gorman, Jason; Anthony, Colin S. Journal of Virology, Vol. 90, Issue 22 https://doi.org/10.1128/JVI.01357-16	journal	August 2016
Removal of a Single N-Linked Glycan in Human Immunodeficiency Virus Type 1 gp120 Results in an Enhanced Ability To Induce Neutralizing Antibody Responses Li, Yun; Cleveland, Bradley; Klots, Igor Journal of Virology, Vol. 82, Issue 2 https://doi.org/10.1128/JVI.01691-07	journal	October 2007
Tiered Categorization of a Diverse Panel of HIV-1 Env Pseudoviruses for Assessment of Neutralizing Antibodies Seaman, M. S.; Janes, H.; Hawkins, N. Journal of Virology, Vol. 84, Issue 3 https://doi.org/10.1128/JVI.02108-09	journal	November 2009
Neutralization Escape Variants of Human Immunodeficiency Virus Type 1 Are Transmitted from Mother to Infant Wu, X.; Parast, A. B.; Richardson, B. A. Journal of Virology, Vol. 80, Issue 2 https://doi.org/10.1128/JVI.80.2.835-844.2006	journal	December 2005
Comprehensive Functional Analysis of N-Linked Glycans on Ebola Virus GP1 Lennemann, Nicholas J.; Rhein, Bethany A.; Ndungo, Esther mBio, Vol. 5, Issue 1 https://doi.org/10.1128/mBio.00862-13	journal	January 2014
Analysis of Memory B Cell Responses and Isolation of Novel Monoclonal Antibodies with Neutralizing Breadth from HIV-1-Infected Individuals Corti, Davide; Langedijk, Johannes P. M.; Hinz, Andreas PLoS ONE, Vol. 5, Issue 1 https://doi.org/10.1371/journal.pone.0008805	journal	January 2010
Serverification of Molecular Modeling Applications: The Rosetta Online Server That Includes Everyone (ROSIE) Lyskov, Sergey; Chou, Fang-Chieh; Conchúir, Shane Ó. PLoS ONE, Vol. 8, Issue 5 https://doi.org/10.1371/journal.pone.0063906	journal	May 2013
The N276 Glycosylation Site Is Required for HIV-1 Neutralization by the CD4 Binding Site Specific HJ16 Monoclonal Antibody Balla-Jhagjhoorsingh, Sunita S.; Corti, Davide; Heyndrickx, Leo PLoS ONE, Vol. 8, Issue 7 https://doi.org/10.1371/journal.pone.0068863	journal	July 2013
Structure-Based Stabilization of HIV-1 gp120 Enhances Humoral Immune Responses to the Induced Co-Receptor Binding Site Dey, Barna; Svehla, Krisha; Xu, Ling PLoS Pathogens, Vol. 5, Issue 5 https://doi.org/10.1371/journal.ppat.1000445	journal	May 2009
A Next-Generation Cleaved, Soluble HIV-1 Env Trimer, BG505 SOSIP.664 gp140, Expresses Multiple Epitopes for Broadly Neutralizing but Not Non-Neutralizing Antibodies Sanders, Rogier W.; Derking, Ronald; Cupo, Albert PLoS Pathogens, Vol. 9, Issue 9, Article No. e1003618 https://doi.org/10.1371/journal.ppat.1003618	journal	September 2013
Comprehensive Antigenic Map of a Cleaved Soluble HIV-1 Envelope Trimer Derking, Ronald; Ozorowski, Gabriel; Sliepen, Kwinten PLOS Pathogens, Vol. 11, Issue 3 https://doi.org/10.1371/journal.ppat.1004767	journal	March 2015
A New Glycan-Dependent CD4-Binding Site Neutralizing Antibody Exerts Pressure on HIV-1 In Vivo Freund, Natalia T.; Horwitz, Joshua A.; Nogueira, Lilian PLOS Pathogens, Vol. 11, Issue 10 https://doi.org/10.1371/journal.ppat.1005238	journal	October 2015
Arenavirus Glycan Shield Promotes Neutralizing Antibody Evasion and Protracted Infection Sommerstein, Rami; Flatz, Lukas; Remy, Melissa M. PLOS Pathogens, Vol. 11, Issue 11 https://doi.org/10.1371/journal.ppat.1005276	journal	November 2015
The Hepatitis C Virus Glycan Shield and Evasion of the Humoral Immune Response Helle, François; Duverlie, Gilles; Dubuisson, Jean Viruses, Vol. 3, Issue 10 https://doi.org/10.3390/v3101909	journal	October 2011

Similar Records

HIV-1 CD4-binding site germline antibody–Env structures inform vaccine design

Journal Article · Sun Oct 16 20:00:00 EDT 2022 · Nature Communications · OSTI ID:2423906

Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer and a glycosylated HIV-1 gp120 core

Journal Article · Tue Nov 06 19:00:00 EST 2018 · eLife · OSTI ID:1628887

Structural basis for germline antibody recognition of HIV-1 immunogens

Journal Article · Sun Mar 20 20:00:00 EDT 2016 · eLife · OSTI ID:1628850

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CD4 binding site
N276
antibody approach angle
antibody-antigen binding
cryo-EM
glycan conformation
glycan276
immunogen design

Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies

Citation Formats

References (81)

Similar Records

Related Subjects