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Redundant roles of four ZIP family members in zinc homeostasis and seed development in Arabidopsis thaliana

Journal Article · · The Plant Journal
DOI:https://doi.org/10.1111/tpj.15506· OSTI ID:1829283
 [1];  [2];  [3];  [4];  [5];  [6];  [4]
  1. Dartmouth College, Hanover, NH (United States); Institute for Basic Science (IBS), Daegu (Korea)
  2. Dartmouth College, Hanover, NH (United States); Duke Kunshan Univeristy, Jiangsu (China)
  3. Dartmouth College, Hanover, NH (United States); Federal Univ. of Rio Grande do Sul, Porto Alegre (Brazil)
  4. Dartmouth College, Hanover, NH (United States)
  5. Brookhaven National Lab. (BNL), Upton, NY (United States)
  6. Univ. of Nottingham (United Kingdom)
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Zinc (Zn) is essential for normal plant growth and development. The Zn-regulated transporter, iron-regulated transporter (IRT)-like protein (ZIP) family members are involved in Zn transport and cellular Zn homeostasis throughout the domains of life. In this study, we have characterized four ZIP transporters from Arabidopsis thaliana (IRT3, ZIP4, ZIP6, and ZIP9) to better understand their functional roles. The four ZIP proteins can restore the growth defect of a yeast Zn uptake mutant and are upregulated under Zn deficiency. Single and double mutants show no phenotypes under Zn-sufficient or Zn-limited growth conditions. In contrast, triple and quadruple mutants show impaired growth irrespective of external Zn supply due to reduced Zn translocation from root to shoot. All four ZIP genes are highly expressed during seed development, and siliques from all single and higher-order mutants exhibited an increased number of abnormal seeds and decreased Zn levels in mature seeds relative to wild type. The seed phenotypes could be reversed by supplementing the soil with Zn. Finally, our data demonstrate that IRT3, ZIP4, ZIP6, and ZIP9 function redundantly in maintaining Zn homeostasis and seed development in A. thaliana.
Research Organization:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Organization:
National Institutes of Health (NIH); National Science Foundation (NSF); USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES)
Grant/Contract Number:
AC02-76SF00515; AC02-98CH10886; FG02-06ER15809; SC0012704
OSTI ID:
1829283
Alternate ID(s):
OSTI ID: 1825129
Report Number(s):
BNL--222345-2021-JAAM
Journal Information:
The Plant Journal, Journal Name: The Plant Journal Journal Issue: 4 Vol. 108; ISSN 0960-7412
Publisher:
Society for Experimental BiologyCopyright Statement
Country of Publication:
United States
Language:
English

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Arabidopsis thaliana
ZIP transporter
abiotic stress
high-order mutants
seeds
zinc
zinc homeostasis