Cytochrome P450–catalyzed biosynthesis of furanoditerpenoids in the bioenergy crop switchgrass (Panicum virgatum L.)

Muchlinski, Andrew; Jia, Meirong; Tiedge, Kira; Fell, Jason S.; Pelot, Kyle A.; Chew, Lisl; Davisson, Danielle; Chen, Yuxuan; Siegel, Justin; Lovell, John T.; Zerbe, Philipp

doi:10.1111/tpj.15492

Cytochrome P450–catalyzed biosynthesis of furanoditerpenoids in the bioenergy crop switchgrass (Panicum virgatum L.)

Journal Article · Mon Sep 13 04:00:00 EDT 2021 · The Plant Journal

DOI:https://doi.org/10.1111/tpj.15492· OSTI ID:1828388

Muchlinski, Andrew ^[1]; Jia, Meirong ^[2]; Tiedge, Kira ^[2]; Fell, Jason S. ^[2]; Pelot, Kyle A. ^[2]; Chew, Lisl ^[2]; Davisson, Danielle ^[2]; Chen, Yuxuan ^[2]; Siegel, Justin ^[2]; Lovell, John T. ^[3]; ^[2]

Univ. of California, Davis, CA (United States); University of California-Davis
Univ. of California, Davis, CA (United States)
Hudson Alpha Inst. for Biotechnology, Huntsville, AL (United States)

Specialized diterpenoid metabolites are important mediators of plant-environment interactions in monocot crops. To understand metabolite functions in plant environmental adaptation that ultimately can enable crop improvement strategies, a deeper knowledge of the underlying species-specific biosynthetic pathways is required. Furthermore, we report the genomics-enabled discovery of five cytochrome P450 monooxygenases (CYP71Z25-CYP71Z29) that form previously unknown furanoditerpenoids in the monocot bioenergy crop Panicum virgatum (switchgrass). Combinatorial pathway reconstruction showed that CYP71Z25-CYP71Z29 catalyze furan ring addition directly to primary diterpene alcohol intermediates derived from distinct class II diterpene synthase products. Transcriptional co-expression patterns and the presence of select diterpenoids in switchgrass roots support the occurrence of P450-derived furanoditerpenoids in planta. Integrating molecular dynamics, structural analysis and targeted mutagenesis identified active site determinants that contribute to the distinct catalytic specificities underlying the broad substrate promiscuity of CYP71Z25-CYP71Z29 for native and non-native diterpenoids.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of California, Davis, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0019178; AC02-05CH11231

OSTI ID:: 1828388

Journal Information:: The Plant Journal, Journal Name: The Plant Journal Journal Issue: 4 Vol. 108; ISSN 0960-7412

Publisher:: Society for Experimental BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Cytochrome P450–catalyzed biosynthesis of furanoditerpenoids in the bioenergy crop switchgrass (Panicum virgatum L.)

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