Hydrothermal pretreatment for valorization of genetically engineered bioenergy crop for lipid and cellulosic sugar recovery

Singh, Ramkrishna; Liu, Hui; Shanklin, John; Singh, Vijay

doi:10.1016/j.biortech.2021.125817

Hydrothermal pretreatment for valorization of genetically engineered bioenergy crop for lipid and cellulosic sugar recovery

Journal Article · Sat Aug 21 00:00:00 EDT 2021 · Bioresource Technology

DOI:https://doi.org/10.1016/j.biortech.2021.125817· OSTI ID:1828307

Singh, Ramkrishna ^[1]; Liu, Hui ^[2]; ^[2]; Singh, Vijay ^[1]

Univ. of Illinois at Urbana-Champaign, IL (United States)
Univ. of Illinois at Urbana-Champaign, IL (United States); Brookhaven National Lab. (BNL), Upton, NY (United States)

Lipids accumulated in the vegetative tissues of cellulosic feedstocks can be a potential raw material for biodiesel and bioethanol production. In this work, bagasse of genetically engineered sorghum was subjected to liquid hot-water pretreatment at 170, 180, and 190°C for different reaction time. Under the optimal pretreatment condition (170°C, 20 min), the residue was enriched in glucan (57.39 ± 2.63 % w/w) and xylan (13.38 ± 0.49 % w/w). The total lipid content of the pretreated residue was 6.81% w/w, similar to that observed in untreated bagasse (6.30% w/w). Pretreatment improved the enzymatic digestibility of bagasse, allowing a recovery of 79% w/w and 86% w/w of glucose and xylose, respectively. The pretreatment and enzymatic saccharification resulted in a 2-fold increase in total lipid in enzymatic residue compared to the original bagasse. Furthermore, pretreatment and enzymatic hydrolysis enabled high sugar recovery while concentrating triglycerides and free fatty acids in the residue.

View Accepted Manuscript (DOE)

Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States); Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0012704; SC0018420

OSTI ID:: 1828307

Alternate ID(s):: OSTI ID: 1834455

Report Number(s):: BNL--222236-2021-JAAM

Journal Information:: Bioresource Technology, Journal Name: Bioresource Technology Vol. 341; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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