Pith–specific lignification in Nicotiana attenuata as a defense against a stem–boring herbivore

Joo, Youngsung; Kim, Hoon; Kang, Moonyoung; Lee, Gisuk; Choung, Sungjun; Kaur, Harleen; Oh, Shinyoung; Choi, Jun Weon; Ralph, John; Baldwin, Ian T.; Kim, Sang‐Gyu

doi:10.1111/nph.17583

Pith–specific lignification in Nicotiana attenuata as a defense against a stem–boring herbivore

Journal Article · Fri Jun 25 00:00:00 EDT 2021 · New Phytologist

DOI:https://doi.org/10.1111/nph.17583· OSTI ID:1827412

Joo, Youngsung ^[1]; Kim, Hoon ^[2]; Kang, Moonyoung ^[3]; Lee, Gisuk ^[4]; ^[3]; Kaur, Harleen ^[5]; Oh, Shinyoung ^[6]; Choi, Jun Weon ^[7]; Ralph, John ^[2]; ^[5]; ^[3]

Max Planck Inst. for Chemical Ecology, Jena (Germany); Korea Advanced Inst. for Science and Technology, Daejeon (Korea); Chungbuk National Univ., Cheongju (Korea); Max Planck Institute for Chemical Ecology Department of Molecular Ecology
Univ. of Wisconsin, Madison, WI (United States)
Korea Advanced Inst. for Science and Technology, Daejeon (Korea)
Max Planck Inst. for Chemical Ecology, Jena (Germany); Korea Advanced Inst. for Science and Technology, Daejeon (Korea)
Max Planck Inst. for Chemical Ecology, Jena (Germany)
Graduate School of International Agricultural Technology Seoul National University Pyoeng‐Chang 25354 Korea
Seoul National Univ., Pyoeng‐Chang (Korea)

Plants have developed tissue-specific defense strategies in response to various herbivores with different feeding habits. Although defense responses to leaf-chewing insects have been well studied, little is known about stem-specific responses, particularly in the pith, to stem-boring herbivores. To understand the stem-specific defense, we first conducted a comparative transcriptomic analysis of the wild tobacco Nicotiana attenuata before and after attack by the leaf-chewing herbivore Manduca sexta and the stem borer Trichobaris mucorea. When the stem-boring herbivore attacked, lignin-associated genes were upregulated specifically in the inner parenchymal cells of the stem, the pith; lignin also accumulated highly in the attacked pith. Silencing the lignin biosynthetic gene cinnamyl alcohol dehydrogenase enhanced the performance of the stem-boring herbivore but had no effect on the growth of the leaf-chewing herbivore. Two-dimensional nuclear magnetic resonance results revealed that lignified pith contains feruloyltyramine as an unusual lignin component in the cell wall, as a response against stem-boring herbivore attack. Pith-specific lignification induced by the stem-boring herbivore was modulated by both jasmonate and ethylene signaling. Furthermore, these results suggest that lignin provides a stem-specific inducible barrier, protecting plants against stem-boring insects.

View Accepted Manuscript (DOE)

Research Organization:: Great Lakes Bioenergy Research Center, Madison, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018409

OSTI ID:: 1827412

Journal Information:: New Phytologist, Journal Name: New Phytologist Journal Issue: 1 Vol. 232; ISSN 0028-646X

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Cinnamyl alcohol dehydrogenase (CAD)
Trichobaris mucorea
ethylene
feruloyltyramine
herbivore-induced lignification
jasmonate
pith
plant defense

Pith–specific lignification in Nicotiana attenuata as a defense against a stem–boring herbivore

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