Colloid-like solution behavior of computationally designed coiled coil bundlemers

Sinha, Nairiti J.; Guo, Rui; Misra, Rajkumar; Fagan, Jeffrey; Faraone, Antonio; Kloxin, Christopher J.; Saven, Jeffery G.; Jensen, Grethe V.; Pochan, Darrin J.

doi:10.1016/j.jcis.2021.09.184

Title: Colloid-like solution behavior of computationally designed coiled coil bundlemers

Journal Article · Sat Jan 01 00:00:00 EST 2022 · Journal of Colloid and Interface Science

DOI:https://doi.org/10.1016/j.jcis.2021.09.184· OSTI ID:1823285

Sinha, Nairiti J.; Guo, Rui; Misra, Rajkumar; Fagan, Jeffrey; Faraone, Antonio; Kloxin, Christopher J.; Saven, Jeffery G.; Jensen, Grethe V.; Pochan, Darrin J.

The use of isotropic potential models of simple colloids for describing complex protein–protein interactions is a topic of ongoing debate in the biophysical community. This contention stems from the unavailability of synthetic protein-like model particles that are amenable to systematic experimental characterization. In this article, we test the utility of colloidal theory to capture the solution structure, interactions and dynamics of novel globular protein-mimicking, computationally designed peptide assemblies called bundlemers that are programmable model systems at the intersection of colloids and proteins. Small-angle neutron scattering (SANS) measurements of semi-dilute bundlemer solutions in low and high ionic strength solution indicate that bundlemers interact locally via repulsive interactions that can be described by a screened repulsive potential. We also present neutron spin echo (NSE) spectroscopy results that show high-Q freely-diffusive dynamics of bundlemers. Importantly, formation of clusters due to short-range attractive, inter-bundlemer interactions is observed in SANS even at dilute bundlemer concentrations, which is indicative of the complexity of the bundlemer charged surface. The similarities and differences between bundlemers and simple colloidal as well as complex protein–protein interactions is discussed in detail.

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Research Organization:: Univ. of Pennsylvania, Philadelphia, PA (United States); Univ. of Delaware, Newark, DE (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institute of Standards and Technology (NIST); US Department of Commerce; National Science Foundation (NSF)

Grant/Contract Number:: SC0019282; SC0019355; DMR-2010792

OSTI ID:: 1823285

Alternate ID(s):: OSTI ID: 1977264

Journal Information:: Journal of Colloid and Interface Science, Journal Name: Journal of Colloid and Interface Science Vol. 606 Journal Issue: P2; ISSN 0021-9797

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
bundlemer
computational design
protein–protein interactions
colloids
small-angle neutron scattering
SANS
neutron spin echo
NSE

Title: Colloid-like solution behavior of computationally designed coiled coil bundlemers

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