Pseudomonas sp. Strain 273 Degrades Fluorinated Alkanes

Xie, Yongchao; Chen, Gao; May, Amanda L.; Yan, Jun; Brown, Lindsay P.; Powers, Joshua B.; Campagna, Shawn R.; Löffler, Frank E.

doi:10.1021/acs.est.0c04029

Pseudomonas sp. Strain 273 Degrades Fluorinated Alkanes

Journal Article · Sun Nov 15 23:00:00 EST 2020 · Environmental Science and Technology

DOI:https://doi.org/10.1021/acs.est.0c04029· OSTI ID:1820754

Xie, Yongchao ^[1]; Chen, Gao ^[2]; May, Amanda L. ^[3]; Yan, Jun ^[4]; Brown, Lindsay P. ^[5]; Powers, Joshua B. ^[5]; Campagna, Shawn R. ^[5]; ^[1]

Univ. of Tennessee, Knoxville, TN (United States); Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Univ. of Tennessee, Knoxville, TN (United States); Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology
Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology
Chinese Academy of Sciences (CAS), Shenyang (China). Inst. of Applied Ecology, Key Lab. of Pollution Ecology and Environmental Engineering
Univ. of Tennessee, Knoxville, TN (United States)

Fluorinated organic compounds have emerged as environmental constituents of concern. We demonstrate that the alkane degrader Pseudomonas sp. strain 273 utilizes terminally monofluorinated C₇–C₁₀ alkanes and 1,10-difluorodecane (DFD) as the sole carbon and energy sources in the presence of oxygen. Strain 273 degraded 1-fluorodecane (FD) (5.97 ± 0.22 mM, nominal) and DFD (5.62 ± 0.13 mM, nominal) within 7 days of incubation, and 92.7 ± 3.8 and 90.1 ± 1.9% of the theoretical maximum amounts of fluorine were recovered as inorganic fluoride, respectively. With n-decane, strain 273 attained (3.24 ± 0.14) × 10⁷ cells per μmol of carbon consumed, while lower biomass yields of (2.48 ± 0.15) × 10⁷ and (1.62 ± 0.23) × 10⁷ cells were measured with FD or DFD as electron donors, respectively. The organism coupled decanol and decanoate oxidation to denitrification, but the utilization of (fluoro)alkanes was strictly oxygen-dependent, presumably because the initial attack on the terminal carbon requires oxygen. Fluorohexanoate was detected as an intermediate in cultures grown with FD or DFD, suggesting that the initial attack on the fluoroalkanes can occur on the terminal methyl or fluoromethyl groups. Overall, the findings indicate that specialized bacteria such as Pseudomonas sp. strain 273 can break carbon–fluorine bonds most likely with oxygenolytic enzyme systems and that terminally monofluorinated alkanes are susceptible to microbial degradation. The findings have implications for the fate of components associated with aqueous film-forming foam (AFFF) mixtures.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1820754

Journal Information:: Environmental Science and Technology, Journal Name: Environmental Science and Technology Journal Issue: 23 Vol. 54; ISSN 0013-936X

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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