Cryo-EM structures of engineered active bc1-cbb3 type CIII2CIV super-complexes and electronic communication between the complexes

Steimle, Stefan; van Eeuwen, Trevor; Ozturk, Yavuz; Kim, Hee Jong; Braitbard, Merav; Selamoglu, Nur; Garcia, Benjamin A.; Schneidman-Duhovny, Dina; Murakami, Kenji; Daldal, Fevzi

doi:10.1038/s41467-021-21051-4

Cryo-EM structures of engineered active bc1-cbb3 type CIII₂CIV super-complexes and electronic communication between the complexes

Journal Article · Wed Feb 10 00:00:00 EST 2021 · Nature Communications

DOI:https://doi.org/10.1038/s41467-021-21051-4· OSTI ID:1816760

Steimle, Stefan ^[1]; ^[2]; Ozturk, Yavuz ^[3]; ^[2]; Braitbard, Merav ^[4]; Selamoglu, Nur ^[5]; Garcia, Benjamin A. ^[6]; Schneidman-Duhovny, Dina ^[4]; ^[6]; ^[5]

Univ. of Pennsylvania, Philadelphia, PA (United States). Dept. of Biology; OSTI
Univ. of Pennsylvania, Philadelphia, PA (United States). Perelman School of Medicine. Biochemistry and Molecular Biophysics Graduate Group
Univ. of Pennsylvania, Philadelphia, PA (United States). Dept. of Biology; Albert-Ludwigs Univ. of Freiburg (Germany). Faculty of Medicine. Inst. of Biochemistry and Molecular Biology
Hewbrew Univ. of Jerusalem (Israel). Inst. of Life Sciences. School of Computer Science and Engineering
Univ. of Pennsylvania, Philadelphia, PA (United States). Dept. of Biology
Univ. of Pennsylvania, Philadelphia, PA (United States). Perelman School of Medicine. Dept. of Biochemistry and Biophysics

Respiratory electron transport complexes are organized as individual entities or combined as large supercomplexes (SC). Gram-negative bacteria deploy a mitochondrial-like cytochrome (cyt) bc₁ (Complex III, CIII₂), and may have specific cbb₃-type cyt c oxidases (Complex IV, CIV) instead of the canonical aa₃-type CIV. Electron transfer between these complexes is mediated by soluble (c₂) and membrane-anchored (c_y) cyts. Here, we report the structure of an engineered bc₁-cbb₃ type SC (CIII₂CIV, 5.2Å resolution) and three conformers of native CIII₂ (3.3 Å resolution). The SC is active in vivo and in vitro, contains all catalytic subunits and cofactors, and two extra transmembrane helices attributed to cyt c_y and the assembly factor CcoH. The cyt c_y is integral to SC, its cyt domain is mobile and it conveys electrons to CIV differently than cyt c₂. The successful production of a native-like functional SC and determination of its structure illustrate the characteristics of membrane-confined and membrane-external respiratory electron transport pathways in Gram-negative bacteria.

View Accepted Manuscript (DOE)

Sponsoring Organization:: German Research Foundation (DFG); Ministry of Science and Technology; NCI; National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division

Grant/Contract Number:: FG02-91ER20052

OSTI ID:: 1816760

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 12; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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