Transcriptional control of Clostridium autoethanogenum using CRISPRi

Fackler, Nicholas; Heffernan, James; Juminaga, Alex; Doser, Damien; Nagaraju, Shilpa; Gonzalez-Garcia, R. Axayacatl; Simpson, Séan D.; Marcellin, Esteban; Köpke, Michael

doi:10.1093/synbio/ysab008

Transcriptional control of Clostridium autoethanogenum using CRISPRi

Journal Article · Tue Feb 09 23:00:00 EST 2021 · Synthetic Biology

DOI:https://doi.org/10.1093/synbio/ysab008· OSTI ID:1816758

^[1]; ^[2]; Juminaga, Alex ^[3]; Doser, Damien ^[3]; Nagaraju, Shilpa ^[3]; Gonzalez-Garcia, R. Axayacatl ^[2]; Simpson, Séan D. ^[3]; Marcellin, Esteban ^[2]; Köpke, Michael ^[3]

LanzaTech Inc, Skokie, IL (United States); OSTI
Univ. of Queensland, Brisbane, QLD (Australia)
LanzaTech Inc, Skokie, IL (United States)

Gas fermentation by Clostridium autoethanogenum is a commercial process for the sustainable biomanufacturing of fuels and valuable chemicals using abundant, low-cost C1 feedstocks (CO and CO₂) from sources such as inedible biomass, unsorted and nonrecyclable municipal solid waste, and industrial emissions. Efforts toward pathway engineering and elucidation of gene function in this microbe have been limited by a lack of genetic tools to control gene expression and arduous genome engineering methods. To increase the pace of progress, here we developed an inducible CRISPR interference (CRISPRi) system for C. autoethanogenum and applied that system toward transcriptional repression of genes with ostensibly crucial functions in metabolism.

View Accepted Manuscript (DOE)

Research Organization:: LanzaTech Inc, Skokie, IL (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0019090; AC02-05CH11231

OSTI ID:: 1816758

Journal Information:: Synthetic Biology, Journal Name: Synthetic Biology Journal Issue: 1 Vol. 6; ISSN 2397-7000

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
CRISPR/Cas9
CRISPRi
Clostridium autoethanogenum
acetogen
gas fermentation

Transcriptional control of Clostridium autoethanogenum using CRISPRi

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References (39)

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