A Comprehensive GC–MS Sub-Microscale Assay for Fatty Acids and its Applications

Bigelow, Nicholas W.; Hardin, William R.; Barker, James P.; Ryken, Scott A.; MacRae, Alex C.; Cattolico, Rose Ann

doi:10.1007/s11746-011-1799-7

A Comprehensive GC–MS Sub-Microscale Assay for Fatty Acids and its Applications

Journal Article · Sun Mar 27 00:00:00 EDT 2011 · Journal of the American Oil Chemists Society

DOI:https://doi.org/10.1007/s11746-011-1799-7· OSTI ID:1816651

Bigelow, Nicholas W. ^[1]; Hardin, William R. ^[2]; Barker, James P. ^[2]; Ryken, Scott A. ^[2]; MacRae, Alex C. ^[3]; Cattolico, Rose Ann ^[2]

Univ. of Washington, Seattle, WA (United States). Dept. of Biology; OSTI
Univ. of Washington, Seattle, WA (United States). Dept. of Biology
Univ. of Washington, Seattle, WA (United States). Dept. of Chemistry

Fatty acid analysis is essential to a broad range of applications including those associated with the nascent algal biofuel and algal bioproduct industries. Current fatty acid profiling methods require lengthy, sequential extraction and transesterification steps necessitating significant quantities of analyte. We report the development of a rapid, microscale, single-step, in situ protocol for GC–MS lipid analysis that requires only 250 lg dry mass per sample. We furthermore demonstrate the broad applications of this technique by profiling the fatty acids of several algal species, small aquatic organisms, insects and terrestrial plant material. When combined with fluorescent techniques utilizing the BODIPY dye family and flow cytometry, this micro-assay serves as a powerful tool for analyzing fatty acids in laboratory and field collected samples, for highthroughput screening, and for crop assessment. Additionally, the high sensitivity of the technique allows for population analyses across a wide variety of taxa.

View Accepted Manuscript (DOE)

Research Organization:: Donald Danforth Plant Science Center, St. Louis, MO (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: EE0003046

OSTI ID:: 1816651

Journal Information:: Journal of the American Oil Chemists Society, Journal Name: Journal of the American Oil Chemists Society Journal Issue: 9 Vol. 88; ISSN 0003-021X

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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A Comprehensive GC–MS Sub-Microscale Assay for Fatty Acids and its Applications

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